Odovtos - International Journal of Dental Sciences ISSN Impreso: 1659-1046 ISSN electrónico: 2215-3411

OAI: https://revistas.ucr.ac.cr/index.php/Odontos/oai
Quantification of Sphingosine-1-Phosphate (S1P) in Patients with Periodontitis Stage II Grade B
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Keywords

Periodontitis; Sphingosine-1-Phosphate; Osteoclastogenesis; Inflammation; Saliva; Gingival crevicular fluid.
Periodontitis; Esfingosina-1-fosfato; Osteoclastogénesis; Inflamación; Saliva; Líquido crevicular gingival.

How to Cite

García-Parra, M. I., Jiménez-Coello, M., Carrillo-Ávila, B. A., Sauri Esquivel, E. A., Martínez-Aguilar, V. M., & Chávez-Cortéz, E. G. (2021). Quantification of Sphingosine-1-Phosphate (S1P) in Patients with Periodontitis Stage II Grade B. Odovtos - International Journal of Dental Sciences, 24(1), 147–155. https://doi.org/10.15517/ijds.2022.48877

Abstract

The aim of this study was the quantification of Sphingosine-1-phosphate (S1P) in periodontal pockets of patients with periodontitis. This is an observational, descriptive, case-control study. Thirty subjects were selected: 15 controls and 15 cases. A periodontal study was conducted following the parameters of AAP 2017 for the diagnosis of periodontal diseases. A sample of saliva and gingival crevicular fluid was obtained from each subject and then analyzed with the Human S1P Elisa kit (MyBioSource #MBS2516132) accordingly to the manufacturer's instructions, in order to verify the presence of S1P and quantify it´s concentration when founded. Results showed a significant difference (p=0.05) between cases and controls. In the case of saliva samples, the concentration of S1P was higher than the ones found in the control group (72.94 ng/mL and 45.12 ng/mL). For GCF, a higher amount of S1P was found in patients with POD (20.09 ng/mL and 15.20 ng/mL). This work raises a possible route of bone metabolism, inflammatory process, and identification of periodontitis through oral quantification of S1P, however, future studies are needed.

https://doi.org/10.15517/ijds.2022.48877
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