Waterpear (Sechium edule) is

an important source of revenue for the exporting countries,

being Costa Rica the leading producer. Among the main

limiting factors for its production are the planting materials,

traditionally the seed, that because of its nature it does not

allow the production of morphologically homogeneous fruits

(color, shape and epidermal texture), which results in high

percentages of rejected fruits at the packing plant. The

establishment of a protocol for vegetative propagation of this

species will help to solve the heterogeneity problems. The

present research was directed to develop a micropropagation

protocol from shoots. The effect of various desinfectants and

concentration of growth regulators during the in vitro

establishment, multiplication and rooting phases were

evaluated. Desinfections with 4% calcium hypochlorite

allowed the highest percentage of aseptically established

explants under in vitro conditions. There was also observed

that during the following phases of micropropagation the

requirements of growth regulators were very low (0.1 mgL-1

BA during bud sprouting and 0.1 to 0.2 mgL-1 AIB during

rooting) or were not required (microcutting multiplication).