Micropropagation of waterpear (Sechium edule Jacq. S.W.) from vegetative shoots.
DOI:
https://doi.org/10.15517/am.v13i2.12070Abstract
Waterpear (Sechium edule) is
an important source of revenue for the exporting countries,
being Costa Rica the leading producer. Among the main
limiting factors for its production are the planting materials,
traditionally the seed, that because of its nature it does not
allow the production of morphologically homogeneous fruits
(color, shape and epidermal texture), which results in high
percentages of rejected fruits at the packing plant. The
establishment of a protocol for vegetative propagation of this
species will help to solve the heterogeneity problems. The
present research was directed to develop a micropropagation
protocol from shoots. The effect of various desinfectants and
concentration of growth regulators during the in vitro
establishment, multiplication and rooting phases were
evaluated. Desinfections with 4% calcium hypochlorite
allowed the highest percentage of aseptically established
explants under in vitro conditions. There was also observed
that during the following phases of micropropagation the
requirements of growth regulators were very low (0.1 mgL-1
BA during bud sprouting and 0.1 to 0.2 mgL-1 AIB during
rooting) or were not required (microcutting multiplication).
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