Morphological, morphometrical and molecular identification of Meloidogyne incognita in fig (Ficus carica L.) in Costa Rica.
Keywords:root-knot nematode, perineal patterns, taxonomy nematode.
The objective of this study was
to identify species of Meloidogyne associated with two fig
plantations in Costa Rica. On March 2012 in Pacayas, Cartago
province, root-galls were found in two fig plantations
of Ficus carica L. Females, egg-masses and juveniles (J2)
of Meloidogyne sp. were extracted from the galled roots.
Female perineal patterns were examined and second infective
stages were analyzed morphometrically and molecularly
by PCR-RFLP. The mitochondrial intergenic region (IGS)
flanked by the cytochrome oxidase subunit II gene (COII)
and the 16S ribosomal gene was amplified. The population
was identified morphologically, morphometrically and molecularly
as M. incognita. The PCR product obtained with
primers C2F3 and 1108 were 1.7 kb in size. When PCR products
were treated with restriction enzymes they generated
four fragments of 850, 450, 250 and 150 bp with AluI and
two fragments of 1300 and 400 bp with HinfI.
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