ía Mesoamericana ISSN electrónico: 2215-3608

Fixed-time artificial insemination in beef cattle with prolonged proestrus of 60 and 72 hours

Darwin Omar Yánez-Avalos, Juan Carlos Lépez Parra, Juan Carlos Moyano Tapia, Roberto Orlando Quinteros Pozo, Pablo Roberto Marini



Fixed- time artificial insemination (IATF, in Spanish) in beef cattle has provided the use of genetically superior bulls in order to maximize the quality of calves. However, implementing this reproductive technique has proved to be difficult to apply in heifers nursing calves for several months of lactancy. An immediate solution to fertility decline of cattle includes the use of hormones. Some hormonal treatments have been developed which are able to control the moment of the first artificial insemination (IA) and the following inseminations in non- pregnant heifers as well as for the treatment of anestrus. The objective of this work was to evaluate two protocols with prolonged proestrus performing artificial insemination at fixed time at 60 or 72 hours in dual purpose cattle from the Ecuadorian Amazon. The study was held from October 2015 to October 2016. A total of 226 multiparious Brows Swiss cattle with nursing calves were inseminated at 60 (T1, n=115) and at 72 (T2, n=111) hours after removing the progesterone device., The conception rate for T1 was 61% (70/115) and for T2: 47% of pregnancy (52/111), showing significant differences (p≤0.05). The estrus was expressed on average by 70.8%, with no significant difference between the protocols (P = 3.15, p≥0.082), for T1 it was 76% (87/115) and for T2 of 65%. The follicular growth at the withdrawal of the progesterone device (T1: 9.53 ± 0.11 mm, T2: 9.87 ± 0.12 mm), the IATF (T1: 12.3 ± 0.12 mm; T2: 11.9 ± 0.12 mm), and the corpus luteum size (T1: 23.1 0.14±mm; T2:22.8 0.15 ±mm) did not show significant differences p≥0.5 between both protocols. It was concluded that both protocols did not show significant differences regarding follicular development at the time of withdrawal of the IATF device and the size of the corpus luteum size measured at day seven after withdrawal. However, the pregnancy rate was higher for the 60-hour protocol.


estradiol; progesterone; ovarian follicles; induced ovulation; pregnancy.


Baruselli, P.S., G.A. Bó, E.L. Rais, O. Marques, e E.F. Sá-Filho. 2005. Introducaosa IATF no manejo reproductivo de rebanhos bovinos de corte no Brasil. Em: M. Caccia, editor, 6º Simposio Internacional de Reproducción Animal. Instituto de Reproducción Animal Córdoba, Córdoba, ARG. p. 151-176.

Bernardi, S., R. Allende, R. Mazeo, J. Monti, y P.R. Marini. 2011. Evaluación de los cambios ocasionados en espermatozoides bovinos por variaciones en el manejo de las dosis durante su manipulación en inseminación artificial. InVet. 13(2):25-39.

Ben, G.A., O.E. Goitia, I.F. Mujica, C.J. Munar, y A.M. Valdez. 2002. Programa de inseminación artificial a tiempo fijo. Manual de procedimientos. Sitio rargentino de reproducción animal, ARG. (consultado 20 jun. 2017).

Bó, G.A., y P.S. Baruselli. 2002. Programas de inseminación artificial a tiempo fijo en el ganado bovino en regiones subtropicales y tropicales. En: C. González-Stagnaro et al., editores, Avances en la ganadería doble propósito. Fundación Girarz, Maracaibo, VEN. p. 499-514.

Bó, G.A., L. Cutaia, G.M. Brogliatti, M. Medina, R. Tríbulo, y H. Tríbulo. 2001. Programas de inseminación artificial a tiempo fijo en ganado bovino utilizando progestágenos y estradiol. En: M. Caccia, editor, Resúmenes, Cuarto Simposio Internacional de Reproducción Animal. Instituto de Reproducción Animal Córdoba,Córdoba, ARG. p. 117-136.

Bridges, G.A., J.K. Ahola, C. Brauner, L.H. Cruppe, J.C. Currin, M.L. Day, P.J. Gunn, J.R. Jaeger, S.L. Lake, G.C. Lamb, G.H. Marquezini, R.K. Peel, A.E. Radunz, J.S. Stevenson, and W.D. Whittier. 2012. Determination of the appropriate delivery of prostaglandin F2α in the five-day CO-Synch + controlled intravaginal drug release protocol in suckled beef cows. J. Anim. Sci. 90:4814-4822. doi: 10.2527/jas.2011-4880.

Bridges, G.A., L.A. Hesler, D.E. Grum, M.L. Mussard, C.L. Gasser, and M.L. Day. 2008. Decreasing the interval between GnRH and PGF2α from 7 to 5 days and lengthening proestros increases timed-IA pregnancy rates in beef cow. Theriogenology 69:843-851. doi:10.1016/j.theriogenology.2007.12.011.

Bridges, G.A., M.L. Mussard, C.R. Burke, and M.L. Day. 2010. Influence of the length of proestros on fertility and endocrine function in female cattle. Anim. Reprod. Sci. 117: 208-215. doi:10.16/j.anireprosci.2009.05.002.

Bridges, G.A., M.L. Mussard, L.A. Hesler, and M.L. Day. 2014. Comparison of follicular dynamics and hormone concentrations between the 7-day and 5-day CO-Synch + CIDR program in primiparous beef cows. Theriogenology 81:632-638. doi:10.1016/j.theriogenology.2013.11.020.

Cerri, R.L., H.M. Rutigliano, R.C. Chebel, and J.P. Santos. 2009. Period of dominance of the ovulatory follicle influences embryo quality in lactating dairy cows. Reproduction 137:813-823. doi:10.1530/REP-08-0242.

Cutaia, L. 2011. Uso sistemático de eCG asociado a protocolos de inseminación artificial a tiempo fijo en ganado de carne y leche. Presentado en: I Simposio Latinoamericano de Reproducción Animal, Hotel Casino Enjoy Viña, Viña del Mar, CHI, 7-8 nov. Presentación 10.

Cutaia, L., G. Veneranda, R. Tribulo, P.S. Barucelli, y G.A. Bó. 2003. Programas de inseminación artificial a tiempo fijo: Análisis de factores que afectan los resultados. En: M. Caccia, editor, V Simposio Internacional de Reproducción Animal. Instituto de Reproducción Animal Córdoba, Córdoba, ARG. p. 119.

de-la-Mata, J.J., y G.A. Bó. 2012. Sincronización de celos y ovulación utilizando protocolos de BE y GnRH en periodos reducidos de inserción de un dispositivo con progesterona en vaquillonas para carne. Taurus 55:17-23.

de-la-Mata, J.J., M. Ré, and G.A. Bó. 2015. Combination of estrus detection and fixed-time artificial insemination in beef heifers following shortened estradiol-based protocol that provides for a lengthened proestrus. Reprod. Fert. Dev. 27:96-97. doi:10.1071/RDV27n1Ab8

Guáqueta, H., J. Zambrano, and C. Jiménez. 2014. Risk factors for ovarian postpartum resumption in Holstein cows, under high tropical conditions. Rev. MVZ Córdoba 19:3970-3983.

Hansel, W., and H.W. Alila. 1984. Causes of postpartum anoestrus in cattle in the tropics. In: International Atomic Energy Agency, editor, Nuclear techniques in tropical animal diseases and nutritional disorders. International Atomic Energy Agency, Vienna, AUT. p. 21-40.

Menchaca, A., R. Nuñez-Olivera, F. Cuadro, and G. Bo. 2015. Pregnancy rates in beef heifers synchronized with a shortened oestradiol-based treatment that provides for a prolonged proestrus. Reprod. Fert. Dev. 27:96. doi:10.1071/RDv27n1Ab7.

Motta-Delgado, P.A., L.G. Rivera-Calderon, A.A. Mariño, and C.E. Lizcano. 2012. Productive and reproductive performance of F1 Gyr x Holstein cows in Colombian warm climate. Vet. Zootec. 6:17-23.

Núñez-Olivera, R., T. de-Castro, C. García-Pintos, G.A. Bó, J. Piaggio, and A. Menchaca. 2014. Ovulatory response and luteal function after eCG administration at the end of a progesterone and estradiol’ based treatment in postpartum anestrous beef cattle. Anim. Reprod. Sci. 146:111-116. doi:10.1016/j.anireprosci.2014.02.017.

Perry, G.A., M.F. Smith, A.J. Roberts, M.D. McNeil, and T.W. Geary. 2007. Relationship between size of the ovulatory follicle and pregnancy success in beef heifers. J. Anim. Sci. 85:684-519. doi:10.2527/jas.2006-519

Pitaluga, P.C.S.F., M.F. Sá-Filho, J.N.S. Sales, P.S. Baruselli, and L. Vincenti. 2013. Manipulation of the proestrous by exogenous gonadotropin and estradiol during a timed artificial insemination protocol in suckled Bos indicus beef cows. Liv. Sci. 154:229-234. doi: 10.1016/j.livsci.2013.02.023.

Sartori, R., P.M. Fricke, J.C. Ferreira, O.J. Ginther, and M.C. Wiltbank. 2001. Follicular deviation and acquisition of ovulatory capacity in bovine follicles. Biol. Reprod. 65:1403-1409.

Van-Eerdenburg, F.J., D. Karthaus, M.A. Taverne, I. Mericsy, and O. Szenci. 2002. The relationship between estrus behavioral score and time of ovulation in dairy cattle. J. Dairy Sci. 85:1150-1156. doi:10.3168/jds.S0022-0302(02)74177-5

Yánez, D., I. Barbona, J.C. López, R. Quinteros, S. Bernardi, and P.R. Marini.2016. Possible factors affecting pregnancy rate of cows in the amazon ecuatorian. In: E. Mellisho, editor, Proceedings VI Peruvian Congress Animal Reproduction. SPERMOVA, PER. p. 66.


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