Sensitivity of Phytophthora infestans to aqueous extracts of Lippia origanoides and Origanum vulgare
DOI:
https://doi.org/10.15517/am.v32i1.40573Keywords:
plant extracts, late blight, Solanum tuberosum, oregano, organic fungicidesAbstract
Introduction. Phytophthora infestans (Mont.) De Bary, is considered the causative agent of one of the most devastating and limiting diseases of potato crops (Solanum tuberosum L.) in the Nariño department (Colombia). This disease is controlled using systemic fungicides, which have a negative impact on the environment and increase production costs, however, there are alternatives like the organic fungicides, which are based on the use of plant extracts with anti-fungal properties for the control of the pathogen. Objective. To evaluate aqueous extracts of O. vulgare and L. origanoides for the control of P. infestans under in vitro conditions. Materials and methods. This research was carried out in the Microbiology laboratory of the research group in Genetics and Evolution of Tropical Organisms (GENPAT) of the Universidad de Nariño, during 2017. The in vitro sensitivity of P. infestans against extracts of botanical species Lippia origanoides (wild oregano) and Origanum vulgare (common oregano) was evaluated. Results. The data obtained allowed the establishment of the base line of behavior of the pathogen populations against the evaluated compounds, there was a gradual reduction in both the growth and sporulation when the Lippia origanoides extract was used and that this same has the capacity to control the pathogen in concentrations of 100 mg ml-1. With the use of Origanum vulgare the P. infestans isolates showed different levels of sensitivity to the compound with low levels of inhibition and high values of EC-50. Conclusion. The P. infestans isolates presented different levels of sensitivity against the two aqueous extracts evaluated, with a reduction of 100 % in the inhibition radial growth and sporulation from the concentration of 100 mg ml-1, when the extract was used of L. origanoides.
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Ahmad, A., Khan, A., Akhtar, F., Yousuf, S., Xess, I., Khan, L. A., & Manzoor, N. (2011). Fungicidal activity of thymol and carvacrol by disrupting ergosterol biosynthesis and membrane integrity against Candida. European journal of clinical microbiology & infectious diseases, 30(1), 41–50. https://doi.org/10.1007/s10096-010-1050-8
Arámbula, C. I., Diaz, C. E., & Garcia, M. I. (2019). Performance, chemical composition and antibacterial activity of the essential oil of Ruta chalepensis and Origanum vulgare. Journal of Physics: Conference Series, 1386(1), 012059. https://doi.org/10.1088/1742-6596/1386/1/012059
Arango, O., Hurtado, A. M., Pantoja, D., & Santacruz, L. (2015). Actividad inhibitoria del aceite esencial de Lippia origanoides H.B.K sobre el crecimiento de Phytophthora infestans. Acta Agronómica, 64(2), 116–124. https://doi.org/10.15446/acag.v64n2.42964.
Arango, O., Hurtado-Benavides, A., & Toro, I. (2011). Efecto del origen, la época de recolección y la edad de las hojas en el rendimiento y el contenido de timol de aceites esenciales de Lippia origanoides HBK. Acta Agronómica, 61(3), 207–213.
Bedoya, O. A., Bolaños, F., Villota, O., Hurtado, A., & Toro, I. (2012). Optimización del rendimiento y contenido de timol de aceite esencial de orégano silvestre obtenido por arrastre con vapor. Biotecnología en el Sector Agropecuario y Agroindustrial, 10(2), 217–226.
Betancur-Galvis, L., Zapata, B., Baena, A., Bueno, J., Ruíz-Nova, C. A., Stashenko, E., & Mesa-Arango, A. C. (2011). Antifungal, cytotoxic and chemical analyses of essential oils of Lippia origanoides HBK grown in Colombia. Revista de la Universidad Industrial de Santander. Salud, 43(2), 141–148.
Busquet, M., Calsamiglia, S., Ferret, A., & Kamel, C. (2005). Screening for effects of plant extracts and active compounds of plants on dairy cattle rumen microbial fermentation in a continuous culture system. Animal Feed Science and Technology, 123–124(2), 597–613. https://doi.org/10.1016/j.anifeedsci.2005.03.008
Dakole, C., Nguefack, J., Dongmo, J., Galani, H., Azah, R., Somda, I., & Amvam, H. (2016). Antifungal potential of essential oils, aqueous and ethanol extracts of thirteen plants against Fusarium oxysporum f. sp Lycopersici and Phytophtora infestans (Mont.) de Bary as major tomato pathogens in Cameroon. International Journal of Current Science, 19(2), 128–145.
Debjani, C., Prerna, D., Seweta, S., Soumen, S., & Susamoy, K. (2018). Role of botanical plant extracts to control plant pathogens-A review. Indian Journal of Agricultural Research, 52(4), 341–346. https://doi.org/10.18805/IJARe.A-5005
Esen, G., Azaz, A. D., Kurkcuoglu, M., Baser, K. H. C., & Tinmaz, A. (2007). Essential oil and antimicrobial activity of wild and cultivated Origanum vulgare L. subsp. hirtum (Link) letswaart from the Marmara region, Turkey. Flavour and Fragrance Journal, 22(5), 371–376. https://doi.org/10.1002/ffj.1808
García, H. G., Marín, M., Jaramillo, S., & Cotes, J. M. (2008). Sensibilidad de aislamientos colombianos de Phytophthora infestans a cuatro fungicidas sistémicos. Agronomía Colombiana, 26(1), 47–57.
García-Beltrán, J. M., & Esteban, M. A. (2016). Properties and applications of plants of Origanum sp. Genus. SM Journal of Biology, 2(1), 1006–1015.
García-Camarillo, E. A., Quezada-Viay, M. Y., Moreno-Lara, J., Sánchez-Hernández, G., Moreno-Martínez, E., & Pérez-Reyes, M. C. J. (2006). Actividad antifúngica de aceites esenciales de canela (Cinnamomum zeylanicum Blume) y orégano (Origanum vulgare L.) y su efecto sobre la producción de aflatoxinas en nuez pecanera [Carya illinoensis (FA Wangenh) K. Koch]. Revista Mexicana de Fitopatología, 24(1), 8-12.
Garrett, K. A., Nelson, R. J., Mundt, C. C., Chacon, G., Jaramillo, R. E., & Forbes, G. A. (2001). The effects of host diversity and other management components on epidemics of potato late blight in the humid highland tropics. Phytopathology, 91(10), 993–1000. https://doi.org/10.1094/PHYTO.2001.91.10.993
International Panel of Experts on Sustainable Food Systems / Food and Agriculture Organization. (2010). Biopreparados para el manejo sostenible de plagas y enfermedades en la agricultura urbana y periurbana. http://www.fao.org/3/a-as435s.pdf
Kim, J., Lee, Y. S., Lee, S. G., Shin, S. C., & Park, I. K. (2008). Fumigant antifungal activity of plant essential oils and components from West Indian bay (Pimenta racemosa) and thyme (Thymus vulgaris) oils against two phytopathogenic fungi. Flavour and Fragrance Journal, 23(4), 272–277. https://doi.org/10.1002/ffj.1882
Mancini, E., Camele, I., Elshafie, H. S., De Martino, L., Pellegrino, C., Grulova, D., & De Feo, V. (2014). Chemical composition and biological activity of the essential oil of Origanum vulgare ssp. hirtum from different areas in the Southern Apennines (Italy). Chemistry & Biodiversity, 11(4), 639–651. https://doi.org/10.1002/cbdv.201300326
Mohsan, M., Ali, S., Shahbaz, M. U., Saeed, S., & Burhan, M. (2017). In vitro efficacy of different growth media and crude plant extracts against mycelia growth of Phytophthora capsici. Journal of Applied Biology & Biotechnology, 5(04), 043–047. https://doi.org/10.7324/JABB.2017.50407
Nagar, D., Biswas, S.K., Singh, M., & Singh, J. (2017). Evaluation of in-vitro Efficacy of Different Plant Extracts against Phytophthora infestans (Mont.) de Bary. International Journal of Current Microbiology and Applied Sciences, 6(6), 412–417. https://doi.org/10.20546/ijcmas.2017.606.048
Nostro, A., Blanco, A. R., Cannatelli, M. A., Enea, V., Flamini, G., Morelli, I., Roccaro, A. S., & Alonzo, V. (2004). Susceptibility of methicillin-resistant staphylococci to oregano essential oil, carvacrol and thymol. FEMS Microbiology Letters, 230(2), 191–195. https://doi.org/10.1016/S0378-1097(03)00890-5
Numpaque, M. A., Oviedo, L. A., Gil, J. H., García, C. M., & Durango, D. L. (2011). Thymol and carvacrol: biotransformation and antifungal activity against the plant pathogenic fungi Colletotrichum acutatum and Botryodiplodia theobromae. Tropical Plant Pathology, 36(1), 3–13. http://dx.doi.org/10.1590/S1982-56762011000100001
Ochoa-Fuentes, Y. M., Cerna-Chávez, E., Landeros-Flores, J., Hernández-Camacho, S., & Delgado-Ortiz, J. C. (2012). Evaluation in vitro of the anti-fungal activity of four methanol plant extracts for the control of three species of Fusarium spp. Phyton, 81, 69–73.
Oliveira, D. R., Leitao, G. G., Bizzo, H. R., Alviano, D. S., Alviano, C. S., & Leitão, S. G. (2007). Chemical and antimicrobial analyses of essential oil of Lippia origanoides HBK. Food Chemistry, 101(1), 236–240. https://doi.org/10.1016/j.foodchem.2006.01.022
Oliveira, D. R., Leitão, G. G., Santos, S. S., Bizzo, H. R., Lopes, D., Alviano, C. S., Alviano, D. S., & Leitão, S. G. (2006). Ethnopharmacological study of two Lippia species from Oriximiná, Brazil. Journal of Ethnopharmacology, 108(1), 103–108. https://doi.org/10.1016/j.jep.2006.04.018
Ramírez, L. S., Isaza, J. H., Veloza, L. Á., Stashenko, E., & Marín, D. (2011). Actividad antibacteriana de aceites esenciales de Lippia origanoides de diferentes orígenes de Colombia. Ciencia, 17(4), 313–321.
Ramírez-González, S. I., López-Báez, O., de Jesús Guzmán-Hernández, T., Munguía-Ulloa, S., & Espinosa-Zaragoza, S. (2011). Actividad antifúngica in vitro de extractos de Origanum vulgare L., Tradescantia spathacea Swartz y Zingiber officinale Roscoe sobre Moniliophthora roreri (Cif & Par) Evans et ál. Revista Tecnología en Marcha, 24(2), 3–17.
Red de Información y Comunicación del Sector Agropecuario de Colombia. (2018). Área, producción y rendimiento nacional por cultivo. https://www.agronet.gov.co/estadistica/Paginas/home.aspx?cod=1
Rivera, H. F., Martínez, E. P., Osorio, J. A., & Martínez, E. (2010). Respuesta de biosurfactantes producidos por Pseudomonas fluorescens para el control de la gota de la papa Phytophthora infestans (Mont) de Bary, bajo condiciones controlada. Ciencia & Tecnología Agropecuaria, 11(1), 21–30. https://doi.org/10.21930/rcta.vol11_num1_art:191
Santa, J. D., Berdugo-Cely, J., Cely-Pardo, L., Soto-Suárez, M., Mosquera, T., & Galeano C. H. (2018). QTL analysis reveals quantitative resistant loci for Phytophthora infestans and Tecia solanivora in tetraploid potato (Solanum tuberosum L.). Plos One, 13(7), e0199716. https://doi.org/10.1371/journal.pone.0199716
Silva, B., Jaramillo, S., & Marín, M. (2009). Caracterización genética de aislamientos de Phytophthora infestans en las zonas productoras de papa de los departamentos de Antioquia, Boyacá, Cundinamarca y Norte de Santander (Colombia). Actualidades Biológicas, 31(90), 5–20.
Soylu, E. M., Soylu, S., & Kurt, S. (2006). Antimicrobial activities of the essential oils of various plants against tomato late blight disease agent Phytophthora infestans. Mycopathologia, 161(2), 119–128. https://doi.org/10.1007/s11046-005-0206-z
Stashenko, E. E., Martínez, J. R., Ruíz, C. A., Arias, G., Durán, C., Salgar, W., & Cala, M. (2010). Lippia origanoides chemotype differentiation based on essential oil GC-MS and principal component analysis. Journal of Separation Science, 33(1), 93–103. https://doi.org/10.1002/jssc.200900452
Stashenko, E. E., & Martínez, R. J. (2019). Study of essential oils obtained from Tropical plants grown in Colombia. In El-Shemy, H. (Ed.), Essential Oils-Oils of Nature. (pp. 403–451). IntechOpen. https://doi.org/10.5772/intechopen.87199
Ultee, A., Bennik, M. H. J., & Moezelaar, R. (2002). The phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen Bacillus cereus. Applied and Environmental Microbiology, 68(4), 1561–1568. https://doi.org/10.1128/AEM.68.4.1561-1568.2002
Vicuña, G. C., Stashenko, E. E., & Fuentes, J. L. (2010). Chemical composition of the Lippia origanoides essential oils and their antigenotoxicity against bleomycin-induced DNA damage. Fitoterapia, 81(5), 343–349. https://doi.org/10.1016/j.fitote.2009.10.008
Wang, K., Jiang, S., Pu, T., Fan, L., Su, F., & Ye, M. (2019). Antifungal activity of phenolic monoterpenes and structure-related compounds against plant pathogenic fungi. Natural Product Research, 33(10), 1423-1430. https://doi.org/10.1080/14786419.2017.1419232
Widmer, T. L., & Laurent, N. (2006). Plant extracts containing caffeic acid and rosmarinic acid inhibit zoospore germination of Phytophthora spp. pathogenic to Theobroma cacao. European Journal of Plant Pathology, 115(4), Article 377. https://doi.org/10.1007/s10658-006-9024-5
You, J., Zhang, J., Wu, M., Yang, L., Chen, W., & Li, G. (2016). Multiple criteria-based screening of Trichoderma isolates for biological control of Botrytis cinerea on tomato. Biological Control, 101, 31–38. https://doi.org/10.1016/j.biocontrol.2016.06.006
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