Cultivo in vitro de Geophila macropoda (Ruiz - PAY. DC) a partir de embriones cigóticos
DOI:
https://doi.org/10.15517/am.v21i1.4913Keywords:
Oreja de ratón, micropropagación, Bencilaminopurina, cobertura vegetal, "Mouse ear", micropropagation, Benzylaminopurine, cover cropAbstract
Cultivo in vitro de Geophila macropoda (Ruiz - Pav. DC) a partir de embriones cigóticos. El objetivo de esta investigación fue desarrollar un protocolo para el cultivo in vitro de Geophila macropoda. Este trabajo se efectuó en Guácimo de Limón, Costa Rica, entre junio del 2006 y febrero del 2007. En la fase de establecimiento in vitro se utilizaron estacas y embriones cigóticos. En el cultivo in vitro, la contaminación bacteriana impidió establecer altos porcentajes de material vegetal. Con embriones se obtuvo hasta del 89%. Para la multiplicación de brotes, se evaluaron cinco concentraciones de bencilaminopurina (BAP). Los mejores resultados se obtuvieron en el medio complementado con 2 mg/l BAP con 13,4 nuevas plántulas a partir de una vitroplanta inicial. El enraizamiento se alcanzó en medio Murashige - Skoog al 50% de las sales recomendadas por tales autores y sin reguladores de crecimiento. Durante la aclimatación se obtuvo una sobrevivencia del 100%.Downloads
Downloads
Published
How to Cite
Issue
Section
License
1. Proposed policy for open access journals
Authors who publish in this journal accept the following conditions:
a. Authors retain the copyright and assign to the journal the right to the first publication, with the work registered under the attribution, non-commercial and no-derivative license from Creative Commons, which allows third parties to use what has been published as long as they mention the authorship of the work and upon first publication in this journal, the work may not be used for commercial purposes and the publications may not be used to remix, transform or create another work.
b. Authors may enter into additional independent contractual arrangements for the non-exclusive distribution of the version of the article published in this journal (e.g., including it in an institutional repository or publishing it in a book) provided that they clearly indicate that the work was first published in this journal.
c. Authors are permitted and encouraged to publish their work on the Internet (e.g. on institutional or personal pages) before and during the review and publication process, as it may lead to productive exchanges and faster and wider dissemination of published work (see The Effect of Open Access).
