Microbiological quality of raw sausage commercialized in the Great Metropolitan Area of Costa Rica
DOI:
https://doi.org/10.15517/am.v34i1.50999Keywords:
meat products, raw pork sausage, microbial pathogens, microbiologyAbstract
Introduction. In Latin America, reports on the microbiological quality of raw chorizo, or raw pork sausage, are limited, however, it is a widely consumed and traditional product. Objective. To determine the microbiological stability of the raw sausage in the Great Metropolitan Area (GAM) of Costa Rica. Materials and methods. Between January and August 2019, microbial shelf-life counts, and contamination indicators were carried out, as well as the detection of three pathogens (Salmonella spp., Listeria monocytogenes, and Clostridium perfringens) in fifty samples of raw chorizo obtained from butcher shops in the Great Metropolitan Area of Costa Rica. Results. Of the fifty samples analyzed, only one was found to be consumable according to the general parameters of bacterial count (total aerobic count [TAM], fungi and yeasts [HYL], and lactic acid bacteria [BAL]). The main cause of spoilage included a combination of BAL and HYL. In addition, the total coliform counts ranged between 105 and 106 UFC/g. From a safety standpoint, Salmonella spp. was isolated in 16 % of the samples, Listeria monocytogenes in 10 % and Clostridium perfringens was found in a single sample with a count of 5.00x101 CFU/g. Conclusion. Non-heat treated products, such as raw chorizo sold in the GAM of Costa Rica, are products that are highly susceptible to spoilage, which present high levels of contamination and that could behave as a vehicle for pathogens; therefore, it is recommended to improve manufacturing practices to prolong shelf life and guarantee consumer safety.
Downloads
References
Al Mamun, M., Mahfuzur Rahman, S. M., & Chowdhury Turin, T. (2013). Microbiological quality of selected street food items vended by school-based street food vendors in Dhaka, Bangladesh. International Journal of Food Microbiology, 166(3), 413–418. https://doi.org/10.1016/j.ijfoodmicro.2013.08.007
Araya-Quesada, Y., Jimenez-Robles, A., Ivankovich-Guillén, C., & García-Barquero, M. (2014). Hábitos de consumo de embutidos en el cantón de San Carlos y el área metropolitana de Costa Rica. Tecnología en Marcha, 27(4), 113–124. https://doi.org/10.18845/tm.v27i4.2091
Becerril Sánchez, A. L., Dublán García, O., Domínguez-López, A., Arizmendi Cotero, D., & Quintero-Salazar, B. (2019). La calidad sanitaria del chorizo rojo tradicional que se comercializa en la ciudad de Toluca, Estado de México. Revista Mexicana de Ciencias Pecuarias, 10(1), 172–185. https://doi.org/10.22319/rmcp.v10i1.4344
Boler, D. D., & Woerner, D. R. (2017). What is meat? A perspective from the American Meat Science Association. Animal Frontiers, 7(4), 8–11. https://doi.org/10.2527/af.2017.0436
Calvo-Arrieta, K., Matamoros-Montoya, K., Arias-Echandi, M. L., Huete-Soto, A., & Redondo-Solano, M. (2021). Presence of Listeria monocytogenes in ready-to-eat meat products sold at retail stores in Costa Rica and analysis of contributing factors. Journal of Food Protection, 84(10), 1729–1740. https://doi.org/10.4315/JFP-21-020
De Cesare, A., Mioni, R., & Manfreda, G. (2007). Prevalence of Listeria monocytogenes in fresh and fermented Italian sausages and ribotyping of contaminating strains. International Journal of Food Microbiology, 120(1–2), 124–130. https://doi.org/10.1016/j.ijfoodmicro.2007.06.009
Ed-Dra, A., Rhazi Filali, F., El Allaoui, A., & Sfendla, A. (2017). Occurrence of Clostridium perfringens in sausages sold in Meknes city, Morocco. Open Veterinary Journal, 7(4), 323–327. https://doi.org/10.4314/ovj.v7i4.6
Food Agriculture Organization. (2020, April). Meat market review. Overview of global meat market developments in 2019. https://www.fao.org/3/ca8819en/CA8819EN.pdf
González-Tenorio, R., Totosaus, A., Caro, I., & Mateo, J. (2013). Caracterización de propiedades químicas y fisicoquímicas de chorizos comercializados en la zona centro de México. Información Tecnológica, 24(2), 3–14.
González Hurtado, M. I., Mesa Gaviria, C. A., & Quintero Cardona, O. A. (2014). Estimación de la vida útil de almacenamiento de carne de res y de cerdo con diferente contenido graso. Vitae, 21(3), 201–210. https://revistas.udea.edu.co/index.php/vitae/article/view/16668
Hachemi, A., Zenia, S., Denia, M. F., Guessoum, M., Hachemi, M. M., & Ait-Oudhia, K. (2019). Epidemiological study of sausage in Algeria: Prevalence, quality assessment, and antibiotic resistance of Staphylococcus aureus isolates and the risk factors associated with consumer habits affecting foodborne poisoning. Veterinary World, 12(8), 1240–1250. https://doi.org/10.14202/vetworld.2019.1240-1250
Heir, E., Møretrø, T., Simensen, A., & Langsrud, S. (2018). Listeria monocytogenes strains show large variations in competitive growth in mixed culture biofilms and suspensions with bacteria from food processing environments. International Journal of Food Microbiology, 275, 46–55. https://doi.org/10.1016/j.ijfoodmicro.2018.03.026
Holck, A., Axelsson, L., McLeod, A., Rode, T. M., & Heir, E. (2017). Health and safety considerations of fermented sausages. Journal of Food Quality, 2017, Article 9753894. https://doi.org/10.1155/2017/9753894
Iulietto, M. F., Sechi, P., Borgogni, E., & Cenci-Goga, B. T. (2015). Meat Spoilage: A Critical Review of a Neglected Alteration Due to Ropy Slime Producing Bacteria. Italian Journal of Animal Science, 14(3), Article e4011. https://doi.org/10.4081/ijas.2015.4011
Jay, J. M., Loessner, M. J., & Golden, D. A. (2005). Modern food microbiology (7th ed.). Springer.
Kalschne, D. L., Geitenes, S., Veit, M. R., Sarmento, C. M. P., & Colla, E. (2014). Growth inhibition of lactic acid bacteria in ham by nisin: A model approach. Meat Science, 98(4), 744–752. https://doi.org/10.1016/j.meatsci.2014.07.002
Kassem, I. I., Nasser, N. A., & Salibi, J. (2020). Prevalence and loads of fecal pollution indicators and the antibiotic resistance phenotypes of Escherichia coli in raw minced beef in lebanon. Foods, 9(11), Article 1543. https://doi.org/10.3390/foods9111543
Kilic, B. (2009). Current trends in traditional Turkish meat products and cuisine. LWT - Food Science and Technology, 42(10), 1581–1589. https://doi.org/10.1016/j.lwt.2009.05.016
Martín-Sánchez, A. M., Chaves-López, C., Sendra, E., Sayas, E., Fenández-López, J., & Pérez-Álvarez, J. Á. (2011). Lipolysis, proteolysis and sensory characteristics of a Spanish fermented dry-cured meat product (salchichón) with oregano essential oil used as surface mold inhibitor. Meat Science, 89(1), 35–44. https://doi.org/10.1016/j.meatsci.2011.03.018
Mendonça Soares, V., Beltrame Padilha, M., de Moraes Guerra, M. E., Alves Schneider, F., Gasparetto, R., Rodrigues dos Santos, E. A., Ereno Tadielo, L., Sperotto Brum, M. C., Kist Traesel, C., & Gonçalves Pereira, J. (2021). Identification of Salmonella spp., Listeria monocytogenes, and indicator microorganisms in commercialized raw meats and fresh sausages from Uruguaiana, Rio Grande do Sul, Brazil. Ciência Rural, 51(6), Article e20200569. https://doi.org/10.1590/0103-8478cr20200569
Miyasaki, K. N., Chiarini, E., de Souza Sant´Ana, A., Destro, M. T., Landgraf, M., & de Melo Franco, B. D. G. (2009). High prevalence, low counts and uncommon serotypes of Listeria monocytogenes in linguiça, a Brazilian fresh pork sausage. Meat Science, 83(3), 523–527. https://doi.org/10.1016/j.meatsci.2009.06.037
Montville, T., & Matthews, K. (2008). Food microbiology: An introduction (2nd ed). ASM Press.
Morera, J., Rodríguez, E., & Gamboa, M. M. (1999). Determinación de Clostridium perfringens en embutidos de carne de cerdo del Área Metropolitana de Costa Rica. Archivos Latinoamericanos de Nutrición, 49(3), 279–282. https://www.alanrevista.org/ediciones/1999/3/art-14/
Mürmann, L., dos Santos, M. C., & Cardoso, M. (2009). Prevalence, genetic characterization and antimicrobial resistance of Salmonella isolated from fresh pork sausages in Porto Alegre, Brazil. Food Control, 20(3), 191–195. https://doi.org/10.1016/j.foodcont.2008.04.007
Odeyemi, O. A., Alegbeleye, O. O., Strateva, M., & Stratev, D. (2020). Understanding spoilage microbial community and spoilage mechanisms in foods of animal origin. Comprehensive Reviews in Food Science and Food Safety, 19(2), 311–331. https://doi.org/10.1111/1541-4337.12526
Organización de Naciones Unidas para la Alimentación y la Agricultura. (2012). Pérdidas y desperdicio de alimentos en el mundo – Alcance, causas y prevención. fao.org/3/i2697s/i2697s.pdf
Pažin, V., Jankuloski, D., Kozačinski, L., Dobranić, V., Njari, B., Cvrtila, Ž., Lorenzo, J. M., & Zdolec, N. (2018). Tracing of Listeria monocytogenes contamination routes in fermented sausage production chain by pulsed-field gel electrophoresis typing. Foods, 7(12), Article 198. https://doi.org/10.3390/foods7120198
Presidencia de la República, Ministerio de Comercio Exterior y de Salud, Ministerio de Agricultura y Ganadería & Ministerio de Economía Industria y Comercio. (2018 diciembre, 21). Reglamento Técnico Centroamericano RTCA 67.04.50:17 Alimentos. Criterios microbiológicos para la inocuidad de los alimentos. La Gaceta. https://www.imprentanacional.go.cr/pub/2018/12/21/ALCA222_21_12_2018.pdf
Programa Estado de la Nación. (2019). Informe Estado de la Nación 2019. https://bit.ly/3VRTtI4
Ramos, D., San Martín, V., Rebatta, M., Arbaiza, T., Salvá, B., Caro, I., & Mateo, J. (2014). Características fisicoquímicas de la salchicha de cerdo del departamento de Tumbes, Perú. Salud y Tecnología Veterinaria, 2(2), 120–128. https://doi.org/10.20453/stv.v2i2.2249
Rodríguez, H., Barreto, G., Sedrés, M., Bertot, J., Martínez, S., & Guevara, G. (2015). Las enfermedades transmitidas por alimentos, un problema sanitario que hereda e incrementa el nuevo milenio. Revista Electrónica de Veterinaria, 16(8), 1–27.
Soares, K., García-Díez, J., Esteves, A., Oliveira, I., & Saraiva, C. (2013). Evaluation of food safety training on hygienic conditions in food establishments. Food Control, 34(2), 613–618. https://doi.org/10.1016/j.foodcont.2013.06.006
Sofos, J. N., Flick, G., Nychas, G. -J., O’Bryan, C. A., Ricke, S. C., & Crandall, P. G. (2013). Meat, poultry, and seafood. In M. P. Doyle, & R. L. Buchanan (Eds.), Food microbiology: fundamentals and frontiers (4th ed., Chapter 6, pp. 109-167). ASM Press. https://doi.org/10.1128/9781555818463.ch6
Spricigo, D. A., Matsumoto, S. R., Espíndola, M. L., & Ferraz, S. M. (2008). Prevalência, quantificação e resistência a antimicrobianos de sorovares de Salmonella isolados de lingüiça frescal suína. Ciência e Tecnologia de Alimentos, 28(4), 779–785. https://doi.org/10.1590/S0101-20612008000400003
Tirado, D., Acevedo, D., & Montero, P. (2015). Calidad microbiológica, fisicoquímica, determinación de nitritos y textura de chorizos comercializados en Cartagena (Colombia). Revista U.D.C.A Actualidad y Divulgación Científica, 18(1), 189–195. https://doi.org/10.31910/rudca.v18.n1.2015.469
Trimoulinard, A., Beral, M., Henry, I., Atiana, L., Porphyre, V., Tessier, C., Leclercq, A., & Cardinale, E. (2017). Contamination by Salmonella spp., Campylobacter spp. and Listeria spp. of most popular chicken- and pork-sausages sold in Reunion Island. International Journal of Food Microbiology, 250, 68–74. https://doi.org/10.1016/j.ijfoodmicro.2017.03.017
Zamora, M. C., & Zaritzky, N. E. (1985). Modeling of microbial growth in refrigerated packaged beef. Journal of Food Science, 50(4), 1003–1006. https://doi.org/10.1111/j.1365-2621.1985.tb12998.x
Zwirzitz, B., Wetzels, S. U., Dixon, E. D., Stessl, B., Zaiser, A., Rabanser, I., Thalguter, S., Pinior, B., Roch, F. -F., Strachan, C., Zanghellini, J., Dzieciol, M., Wagner, M., & Selberherr, E. (2020). The sources and transmission routes of microbial populations throughout a meat processing facility. Npj Biofilms and Microbiomes, 6(1), 26–31. https://doi.org/10.1038/s41522-020-0136-z
Downloads
Additional Files
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Mauricio Redondo-Solano, Valery Cordero-Calderón, Adriana Araya-Morice
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
1. Proposed policy for open access journals
Authors who publish in this journal accept the following conditions:
a. Authors retain the copyright and assign to the journal the right to the first publication, with the work registered under the attribution, non-commercial and no-derivative license from Creative Commons, which allows third parties to use what has been published as long as they mention the authorship of the work and upon first publication in this journal, the work may not be used for commercial purposes and the publications may not be used to remix, transform or create another work.
b. Authors may enter into additional independent contractual arrangements for the non-exclusive distribution of the version of the article published in this journal (e.g., including it in an institutional repository or publishing it in a book) provided that they clearly indicate that the work was first published in this journal.
c. Authors are permitted and encouraged to publish their work on the Internet (e.g. on institutional or personal pages) before and during the review and publication process, as it may lead to productive exchanges and faster and wider dissemination of published work (see The Effect of Open Access).