@article{Artavia-Carmona_Peraza-Padilla_2020, title={Morphological, morphometric and molecular identification of Meloidogyne exigua (Göeldi 1887) in coffee (Coffea arabica)}, volume={31}, url={https://revistas.ucr.ac.cr/index.php/agromeso/article/view/38733}, DOI={10.15517/am.v31i3.38733}, abstractNote={<p><strong><span class="Texto-en-negrita">Introduction.</span></strong><span class="Texto-en-negrita CharOverride-9"><strong> </strong>The genus </span><span class="Texto-en-negrita CharOverride-10">Meloidogyne </span><span class="Texto-en-negrita CharOverride-9">contains many species and their correct diagnosis of these must be based on the use of multiple tools, which in diagnostic laboratories allow a correct and reliable evaluation, specially for quarantine nematodes. The proper identification of a phytonematode is important not only to establish an appropriate control strategy, but also to avoid its spread to other areas where it could cause damage that would result in economic losses.</span><span class="Texto-en-negrita"> <strong>Objective.</strong> </span><span class="Texto-en-negrita CharOverride-9">To perform taxonomic and molecular studies to confirm the </span><span class="Texto-en-negrita CharOverride-10">Meloidogyne </span><span class="Texto-en-negrita CharOverride-9">species, associated to a coffee plantation located in Barva, Heredia. </span><strong><span class="Texto-en-negrita">Materials and methods. </span></strong><span class="Texto-en-negrita CharOverride-9">Soil and root samples were collected in a coffee plantation located in Barva, Heredia, Costa Rica, which were processed in the Nematology laboratory of the School of Agricultural Sciences at the Universidad Nacional (UNA), using the centrifugation-flotation method in sugary solution and molecular techniques. </span><strong><span class="Texto-en-negrita">Results. </span></strong><span class="Texto-en-negrita CharOverride-9">On average, 30 J</span><span class="Texto-en-negrita CharOverride-11">2</span><span class="Texto-en-negrita CharOverride-9"> 100 cc</span><span class="Texto-en-negrita CharOverride-12">-1</span><span class="Texto-en-negrita CharOverride-9"> were counted in soil and 1000 J</span><span class="Texto-en-negrita CharOverride-11">2</span><span class="Texto-en-negrita CharOverride-9"> 10 g</span><span class="Texto-en-negrita CharOverride-12">-1</span><span class="Texto-en-negrita CharOverride-9"> of roots of the root-knot nematode. Taxonomic and molecular techniques allowed to identify that the females and juveniles extracted from coffee roots corresponded to </span><span class="Texto-en-negrita CharOverride-10">Meloidogyne exigua</span><span class="Texto-en-negrita CharOverride-9">. Analysis of the sequences generated by PCR and the use of the DraI enzyme confirmed the identity of the species with 99 % similarity when compared to sequences from Costa Rica and Nicaragua, reported in the Genbank. </span><strong><span class="Texto-en-negrita">Conclusion. </span></strong><span class="Texto-en-negrita CharOverride-9">Using different morphometric and molecular techniques, </span><span class="Texto-en-negrita CharOverride-10">M. exigua</span><span class="Texto-en-negrita CharOverride-9"> associated with a coffee plantation was identified.</span></p>}, number={3}, journal={Agronomía Mesoamericana}, author={Artavia-Carmona, Roy and Peraza-Padilla, Walter}, year={2020}, month={Sep.}, pages={531–545} }