@article{Vega Rodríguez_Hernández-Chaverri_2020, title={Trichoderma growth in pineapple stubble to obtain spores for agricultural use}, volume={31}, url={https://revistas.ucr.ac.cr/index.php/agromeso/article/view/40275}, DOI={10.15517/am.v31i3.40275}, abstractNote={<p><strong><span class="CharOverride-9">Introduction. </span></strong>The use of biological controllers as alternatives to traditional chemicals is an ecological and economic option for farmers. Fungi of the genus <span class="CharOverride-10">Trichoderma</span> can confer resistance to plants and increase the absorption of nutrients, reducing a wide variety of crop diseases. <strong><span class="CharOverride-9">Objective. </span></strong>To evaluate pineapple stubble wastes as a substrate for the solid fermentation growth of two Trichoderma strains to produce a spore solution for agricultural purposes. <strong><span class="CharOverride-9">Materials and methods. </span></strong>The experiment was carried out at the Laboratory of Research in Chemistry and Applied Biosciences of the Universidad Tecnica Nacional of Costa Rica, in the period between April 2018 and October 2019. Two variants of fungus<span class="CharOverride-10"> T. viride</span> and <span class="CharOverride-10">T. harzianum</span> were used, these were grown in natural potato dextrose agar (PDA) and pineapple stubble. A conidia count was performed in a 1/10 mm Neubauer chamber. <span class="CharOverride-9"><strong>Results.</strong> </span><span class="CharOverride-10">T. viride </span>and <span class="CharOverride-10">T. harzianum</span> showed uniform growth in reseeding plates with color changes in time, from white to green. <span class="CharOverride-10">T. viride</span> showed better development in wet substrate, while <span class="CharOverride-10">T. harzianum</span> in low moisture stubble. The maximum cell concentration in the liquid solution of <span class="CharOverride-10">T. harzianum</span> and <span class="CharOverride-10">T. viride</span> was 1 132 750 cell ml<span class="CharOverride-8">-1</span> and 1 023 250 cell ml<span class="CharOverride-8">-1</span>, respectively. <span class="CharOverride-9"><strong>Conclusion.</strong> </span>Both strains of <span class="CharOverride-10">Trichoderma</span> developed adequately in the pineapple stubble, however, the efficient growth of the strains is associated with the management of external factors such as the amount of substrate, oxygen, and moisture.</p>}, number={3}, journal={Agronomía Mesoamericana}, author={Vega Rodríguez, María Catalina and Hernández-Chaverri, Rodolfo Antonio}, year={2020}, month={Sep.}, pages={597–608} }