TY - JOUR AU - Durán-Hernández, Dagoberto AU - Gutiérrez-Hernández, Germán Fernando AU - Arellano-Vázquez, José Luis AU - García-Ramírez, Elpidio AU - Virgen-Vargas, Juan PY - 2011/06/01 Y2 - 2024/03/29 TI - Molecular characterization and seed germination of blue corn landraces with accelerated aging. JF - Agronomía Mesoamericana JA - Agron. Mesoam. VL - 22 IS - 1 SE - Articles DO - 10.15517/am.v22i1.11833 UR - https://revistas.ucr.ac.cr/index.php/agromeso/article/view/11833 SP - 11-20 AB - <p>The objective</p><p>of this work was to determine the effect of accelerated aging</p><p>on seeds of blue corn landraces. The study was conducted in</p><p>the laboratorie s of National Polytechnic Institute and National</p><p>University of México. See ds of four blue corn landraces</p><p>(Cuijingo, Cocotitlán, Puebla and Oaxaca), were subjected</p><p>to accelerated aging treatments: humid heat (HH, 41°C, 72</p><p>h, and 100% r. h.), and dry heat (DH, 60°C, 48 h. The parameters</p><p>measured were: radicle protrusion (RP) in at 0 to 72</p><p>h of imbibition, and after seven days of incubation at 25°C,</p><p>normal seedlings (NS ), abnormal seedlings (AS ), and dead</p><p>seeds (DS) were counted. The molecular characterization by</p><p>RA PD was also performed and the respective dendrograms</p><p>were constructed. In normal seedlings obtained from seeds</p><p>without accelerated ageing, the varie tie s Oaxaca and Puebla</p><p>were significantly superior. Seedling dry weight diminished</p><p>with deterioration, being minimum under dry heat. The number</p><p>of abnormal seedlings increased under both deterioration</p><p>treatments, in the order Oaxaca, Puebla, Cocotitlán and</p><p>Cuijingo. Differentiation of the band profiles among control</p><p>and deteriorated seeds occurred in the sequence: Oaxaca</p><p>(31,6%), Cocotitlán (25,8%), Puebla (19,6%) and Cuijingo</p><p>(18,6%). These similarity values were inversely related to</p><p>seed vigour, and irectly related to the presence of abnormalitie</p><p>s in seedlings.</p> ER -