TY - JOUR AU - Abdelnour Esquivel, Ana AU - Ramírez, Carlos AU - Engelmann, Florent PY - 2006/07/01 Y2 - 2024/03/29 TI - Micropropagation of waterpear (Sechium edule Jacq. S.W.) from vegetative shoots. JF - Agronomía Mesoamericana JA - Agron. Mesoam. VL - 13 IS - 2 SE - Articles DO - 10.15517/am.v13i2.12070 UR - https://revistas.ucr.ac.cr/index.php/agromeso/article/view/12070 SP - 147-151 AB - <p>Waterpear (Sechium edule) is</p><p>an important source of revenue for the exporting countries,</p><p>being Costa Rica the leading producer. Among the main</p><p>limiting factors for its production are the planting materials,</p><p>traditionally the seed, that because of its nature it does not</p><p>allow the production of morphologically homogeneous fruits</p><p>(color, shape and epidermal texture), which results in high</p><p>percentages of rejected fruits at the packing plant. The</p><p>establishment of a protocol for vegetative propagation of this</p><p>species will help to solve the heterogeneity problems. The</p><p>present research was directed to develop a micropropagation</p><p>protocol from shoots. The effect of various desinfectants and</p><p>concentration of growth regulators during the in vitro</p><p>establishment, multiplication and rooting phases were</p><p>evaluated. Desinfections with 4% calcium hypochlorite</p><p>allowed the highest percentage of aseptically established</p><p>explants under in vitro conditions. There was also observed</p><p>that during the following phases of micropropagation the</p><p>requirements of growth regulators were very low (0.1 mgL<span>-1</span></p><p>BA during bud sprouting and 0.1 to 0.2 mgL<span>-1 </span>AIB during</p><p>rooting) or were not required (microcutting multiplication).</p> ER -