TY - JOUR AU - Toledo Perdomo, Claudia Elizabeth AU - Skoda, Steven R. PY - 2020/05/01 Y2 - 2024/03/29 TI - Molecular study of six strains of Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) and Cochliomyia macellaria JF - Agronomía Mesoamericana JA - Agron. Mesoam. VL - 31 IS - 2 SE - Articles DO - 10.15517/am.v31i2.38308 UR - https://revistas.ucr.ac.cr/index.php/agromeso/article/view/38308 SP - 433-444 AB - <p><strong><span class="CharOverride-2">Introduction. </span></strong>The cattle borer worm, <span class="CharOverride-5">Cochliomyia hominivorax</span> (Coquerel), is an economically important pest for warm-blooded animals, mainly cattle. <span class="CharOverride-2"><strong>Objective.</strong> </span>Molecularly characterize six strains of <span class="CharOverride-5">Cochliomyia hominivorax </span>(Coquerel) and the <span class="CharOverride-5">Cochliomyia macellaria</span> species using the mtDNA sequences. <strong><span class="CharOverride-2">Materials and methods. </span></strong>The study was conducted during 2006 and 2007, using six strains from colonies of Mexico, Panama, and Costa Rica, located in the Molecular Biology Laboratory of the Smithsonian Tropical Research Institute, Panama. Three out of six primers were optimized and evaluated, using the cyclic sequencing method. Parsimonia, NJ (Neighbor-Joining), Maximum Likelihood, and Bayesian analyzes were performed to establish the phylogenetic relationships between the strains. Three primers used for <span class="CharOverride-5">C. hominivorax </span>were evaluated for <span class="CharOverride-5">C. macellaria</span>. <strong><span class="CharOverride-2">Results.</span></strong> The regions of the mitochondrial genome NADH4-tRNA-NADH5 were obtained. All the analyzes showed the same topology forming four defined clades. The first formed by the strains from Oaxaca and Quintana Roo, the second by the strains from Chiapas, the third by the strains from Costa Rica and Panama, and the fourth by the population from Brazil, obtained from GenBank. In <span class="CharOverride-5">C. macellaria </span>two primers were able to amplify the NADH4 region and one NADH5 region.<strong><span class="CharOverride-2"> Conclusion. </span></strong>The amplifications obtained in this research provide information to continue the molecular studies between the species of <span class="CharOverride-5">C. macellaria </span>and <span class="CharOverride-5">C. hominivorax</span>; also to identify the geographical origin of the strains.</p> ER -