TY - JOUR AU - La O-Hechavarría, María AU - Zardón-Navarro, María De los Ángeles AU - Arencibia-Rodríguez, Ariel AU - Rodríguez-Lem, Eida AU - Acevedo-Rojas, Ricardo AU - Mesa-López, José María PY - 2011/06/01 Y2 - 2024/03/29 TI - Techniques for the study of sugarcane- S. scitamineum interaction. JF - Agronomía Mesoamericana JA - Agron. Mesoam. VL - 22 IS - 1 SE - Analyzes and Comments DO - 10.15517/am.v22i1.8679 UR - https://revistas.ucr.ac.cr/index.php/agromeso/article/view/8679 SP - 157-165 AB - The main goal of this work was to review<br />the tools and laboratory techniques employed to study<br />the interaction of sugarcane and Sporisorium scitamineum<br />(Syd.) M. Piepe nbr., M. Stoll &amp; F. Oberw. (Ustilago<br />scitaminea Sydow), smut’s causal agent. The sugarcane<br />resistance to this disease is determined by several factors,<br />among them the activation of several resistance proteins, and<br />other related genes. This interaction has been studied with<br />different tools, such as histological techniques for assessing<br />the disruptions at the cellular level caused by fungus<br />penetration; the biochemical ones used to detect changes<br />in the composition of several antimicrobial substances, the<br />histochemistry useful to stain in situ specific proteins, and<br />molecular biology, which provides us with valuable tools to<br />discover the activation or suppression of transcripts during<br />the interaction. These approaches contribute to the better<br />understandings of plant defense responses and to create<br />molecular bases for genetic improvement to be applied as<br />indicator in molecular assisted programs. ER -