From the aerial parts of Zanthoxylum acuminatum (Rutaceae) collected in Upala, Alajuela, Costa Rica were isolated the known lignans hinokinin, (+)-asarinin, (+)- piperitol, (+)-kobusin, and epi-kobusin. In addition to the lignans were isolated the triterpen lupeol and the steroid campesterol. All the separations were performed with chromatographic techniques and the structures were elucidated using 1D and 2D NMR techniques.