Biofilm and Enamel Changes after Interproximal Reduction and Polishing

Orozco Molina, Grissel Guadalupe; Ramírez González, María Fernanda; Pérez Estrada, Yuridia; Pacheco Rondón, Gibeth Steffanni; Armella-Sanchez, Ninelitzetl Sara; Flores-Ledesma, Abigailt; Villegas-Mercado, Carlos Esteban; Bermúdez, Mercedes; Santana-Delgado, Sandra Aidé; Gonzalez-Acosta, Adolfo; Arenas-Alatorre, Jesús; Martínez-Aguilar, Víctor Manuel; Arreguin-Cano, Juan Antonio

doi:10.15517/mw6h1486

Authors

Grissel Guadalupe Orozco Molina Universidad del Valle de Guerrero. Chilpancingo de los Bravo, Guerrero, México. Author https://orcid.org/0009-0002-4808-7849
María Fernanda Ramírez González Universidad del Valle de Guerrero. Chilpancingo de los Bravo, Guerrero, México. Author https://orcid.org/0000-0002-5101-0255
Yuridia Pérez Estrada Universidad del Valle de Guerrero. Chilpancingo de los Bravo, Guerrero, México. Author https://orcid.org/0009-0008-2857-2592
Gibeth Steffanni Pacheco Rondón Universidad del Valle de Guerrero. Chilpancingo de los Bravo, Guerrero, México. Author https://orcid.org/0009-0004-7198-6687
Ninelitzetl Sara Armella-Sanchez Universidad del Valle de Guerrero. Chilpancingo de los Bravo, Guerrero, México. Author https://orcid.org/0009-0009-9463-5982
Abigailt Flores-Ledesma Facultad de Estomatología, Benemérita Universidad Autónoma de Puebla, Puebla, México. Author https://orcid.org/0000-0002-8136-4820
Carlos Esteban Villegas-Mercado Facultad de Odontología. Universidad Autónoma de Chihuahua. Chihuahua, México. Author https://orcid.org/0000-0001-5729-4677
Mercedes Bermúdez Facultad de Odontología. Universidad Autónoma de Chihuahua. Chihuahua, México. Author https://orcid.org/0000-0002-6145-1134
Sandra Aidé Santana-Delgado Facultad de Odontología. Universidad Autónoma de Chihuahua. Chihuahua, México. Author https://orcid.org/0000-0001-7340-1196
Adolfo Gonzalez-Acosta Facultad de Odontología. Universidad Autónoma de Chihuahua. Chihuahua, México. Author https://orcid.org/0009-0008-6565-6509
Jesús Arenas-Alatorre Laboratorio Central de Microscopia, Instituto de Física, Universidad Nacional Autónoma de México, Ciudad de México, México. Author https://orcid.org/0000-0002-5710-4914
Víctor Manuel Martínez-Aguilar Departamento de Especialización en Periodoncia, Facultad de Odontología, Universidad Autónoma de Yucatán, Mérida, México. Author https://orcid.org/0000-0003-2188-5293
Juan Antonio Arreguin-Cano Facultad de Odontología. Universidad Autónoma de Chihuahua. Chihuahua, México. Author https://orcid.org/0000-0003-1292-9880

DOI:

https://doi.org/10.15517/mw6h1486

Keywords:

Interproximal enamel reduction; Stripping; Enamel damage; Post-stripping polishing; Dental biofilm.

Abstract

This study examined the effects of three different interproximal enamel reduction (IER) techniques and subsequent polishing methods on enamel roughness and biofilm formation. Enamel samples were treated with diamond burs, abrasive strips, or diamond discs, followed by polishing using various techniques. Surface roughness was measured with Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), and Profilometry (PM). Biofilm formation of Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans were evaluated in vitro after IER and polishing. The results shown SEM, AFM, and PM revealed increased roughness on enamel surfaces treated with all IER techniques, with PM showing higher Ra and Rz values for the diamond bur IER method. Polishing was performed after IER, reducing roughness across all methods, though not to control levels. Additionally, a significant increase in the levels of S. mutans, L. acidophilus, and C. albicans was observed on enamel surfaces treated with all IER techniques, with reductions seen after polishing.While IER effectively addresses dental crowding, it inherently increases enamel roughness and consequently elevates the risk of biofilm accumulation and caries. This study emphasizes the importance of meticulous polishing procedures and highlights the need for long-term clinical studies to evaluate the enduring effects of IER on oral health.

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Author Biography

Juan Antonio Arreguin-Cano, Facultad de Odontología. Universidad Autónoma de Chihuahua. Chihuahua, México.

LABORATORIO DE INMUNOLOGIA Y METABOLISMO

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