Biocompatibility of Bioactive Sealers Bio-C Sealer vs MTA Repair HP in Human Fibroblasts
DOI:
https://doi.org/10.15517/ijds.2024.58908Keywords:
Bio-C® Sealer; MTA HP® repair; Cytotoxic; Genotoxicity; Cements; Tricalcium silicate.Abstract
Bioactive cements based on tricalcium silicate have been introducedto the market for use in dentistry, with a variety of clinical applications. These cements are in contact with vital tissues such as dental pulp or periodontium in cases of unintentional extrusion; thus, it is important to know the genotoxicity and cytoxicity of these materials. The objective of this study was to evaluate the cytotoxicity and genotoxicity of bioactive sealers, Bio-C® Sealer and MTA Repair HP®, in human fibroblasts. Discs of bioactive sealers Bio-C® Sealer, and MTA Repair HP®, were prepared and set for 24h under sterile conditions. The discs were placed in culture medium at 2.5mg/mL inside a SRT6D roller mixer (Stuart, UK) at 60rpm for 24h. The eluates obtained were incubated for 24h with previously activated and cultured ATCC cell line fibroblasts at 80% confluence. The cytotoxicity was evaluated by Alamar Blue® and LIVE/DEAD assays, as well as the analysis of the Tunel and Mitotracker assays to evaluate genotoxicity using the confocal laser-scanning microscope. In the Alamar Blue® assay, the Bio-C® Sealer presented a cell proliferation of 87%, while the MTA Repair HP® Sealer was 72%. A statistically significant difference was found between the MTA Repair HP® Sealant and the negative control (p=<0.001). Regarding the genotoxicity tests, in the Tunel assay, both materials stain the nucleus of the fibroblast cells exposed to the eluates, while in the Mitotracker assay, the MTA Repair HP® Sealer showed greater mitochondrial function than the Bio-C® Sealer. Calcium silicate-based sealers, Bio-C® Sealer and MTA Repair HP®, are not cytotoxic and have low genotoxicity.
Downloads
References
Wang Z. Bioceramic materials in endodontics. Endod Topics 2015; 32 (1): 3-30. https://doi.org/10.1111/etp.12075.
Garcez A.S., Ribeiro M.S., Tegos G.P., Núñez S.C., Jorge A.O.C., Hamblin M.R. Antimicrobial photodynamic therapy combined with conventional endodontic treatment to eliminate root canal biofilm infection. Lasers Surg Med 2007; 39 (1): 59-66. doi:10.1002/lsm.20415
Bhat A., Cvach N., Mizuno C., Ahn C., Zhu Q., Primus C., et al. Ion release from prototype surface pre-reacted glass ionomer sealer and EndoSequence BC sealer. Eur Endod J 2021; 6 (1): 122-7. doi:10.14744/eej.2020.50470
Komabayashi T., Colmenar D., Cvach N., Bhat A., Primus C., Imai Y. Comprehensive review of current endodontic sealers. Dent Mater J 2020; 39 (5): 703-20. doi:10.4012/dmj.2019-288
Gasner N.S., Brizuela M. Endodontic materials used to fill root canals. In: StatPearls. StatPearls Publishing 2022.
Orstavik D. Materials used for root canal obturation: technical, biological and clinical testing. Endod Topics 2005; 12 (1): 25-38.
Cintra L.T.A., Benetti F., de Azevedo Queiroz Í.O., de Araújo Lopes J.M., Penha de Oliveira S.H., Sivieri Araújo G., et al. Cytotoxicity, biocompatibility, and biomineralization of the new high-plasticity MTA. J Endod 2017; 43 (5): 774-8. doi:10.1016/j.joen.2016.12.018
Parirokh M., Torabinejad M. Mineral trioxide aggregate: A comprehensive literature rreview-Part III: Clinical applications, drawbacks, and mechanism of action. J Endod 2010; 36 (3): 400-13. doi:10.1016/j.joen.2009.09.009
Prati C., Gandolfi M.G. Calcium silicate bioactive cements: Biological perspectives and clinical applications. Dental Materials 2015; 31 (4): 351-70. doi:10.1016/j.dental.2015.01.004
Zordan-Bronzel C.L., Tanomaru-Filho M., Chávez-Andrade G.M., Torres F.F.E., Abi-Rached G.P.C., Guerreiro-Tanomaru J.M. Calcium Silicate-based experimental sealers: Physicochemical properties evaluation. Mat. Res. 2021; 24 (1). https://doi.org/10.1590/1980-5373-MR-2020-0243
López-García S., Lozano A., Garcíabernal D., Forner L., Llena C., Guerrero-Gironés J., et al. Biological effects of new hydraulic materials on human periodontal ligament stem cells. J Clin Med 2019; 8 (8): 1216. doi:10.3390/jcm8081216
Sultana N., Singh M., Nawal R.R., Chaudhry S., Yadav S., Mohanty S., et al. Evaluation of biocompatibility and osteogenic potential of tricalcium silicate-based cements using human bone marrow-derived mesenchymal stem cells. J Endod 2018; 44 (3): 446-51. doi:10.1016/j.joen.2017.11.016
Alves Silva E.C., Tanomaru-Filho M., da Silva G.F., Delfino M.M., Cerri P.S., Guerreiro-Tanomaru J.M. Biocompatibility and bioactive potential of new calcium silicate-based endodontic sealers: Bio-C sealer and sealer plus BC. J Endod 2020; 46 (10): 1470-7. doi:10.1016/j.joen.2020.07.011
Donnermeyer D., Bürklein S., Dammaschke T., Schäfer E. Endodontic sealers based on calcium silicates: a systematic review. Odontology 2019; 107 (4): 421-36. doi:10.1007/s10266-018-0400-3
Meschi N., Patel B., Ruparel N.B. Material Pulp Cells and Tissue Interactions. J Endod 2020; 46 (9): S150-60. doi:10.1016/j.joen.2020.06.031
Rodríguez-Lozano F.J., López-García S., García-Bernal D., Tomás-Catalá C.J., Santos J.M., Llena C., et al. Chemical composition and bioactivity potential of the new Endosequence BC Sealer formulation HiFlow. Int Endod J 2020; 53 (9): 1216-1228. doi:10.1111/iej.13327
Standarization IO for. ISO 10993-1:2009. In: Biological Evaluation of Medical Devices. 2009.
Okamura T., Chen L., Tsumano S., Ikeda C., Komasa S., Tominaga K., et al. Biocompatibility of a high-plasticity, calcium silicate-based, ready-to-use material. Materials 2020; 13 (21): 4770. doi:10.3390/ma13214770
Bonnier F., Keating M.E., Wróbel T.P., Majzner K., Baranska M., Garcia-Munoz A., et al. Cell viability assessment using the Alamar blue assay: A comparison of 2D and 3D cell culture models. Toxicol in Vitro 2015; 29 (1): 24-131. doi: 10.1016/j.tiv.2014.09.014
Longhin E.M., El Yamani N., Rundén-Pran E., Dusinska M. The alamar blue assay in the context of safety testing of nanomaterials. Front Toxicol 2022; 4. doi:10.3389/ftox.2022.981701
McGaw L.J., Elgorashi E.E., Eloff J.N. Cytotoxicity of African Medicinal Plants Against Normal Animal and Human Cells. In: Toxicological Survey of African Medicinal Plants Elsevier; 2014. p. 181-233.
Klein-Junior C.A., Zimmer R., Dobler T., Oliveira V., Marinowic D.R., Özkömür A., et al. Cytotoxicity assessment of bio-c repair Íon: A new calcium silicate-based cement. J Dent Res Dent Clin Dent Prospects 2021; 15 (3): 152-6. doi:10.34172/joddd.2021.026
Abbaszadeh Z., Çeşmeli S., Biray Avcı Ç. Crucial players in glycolysis: Cancer progress. Gene 2020; 726: 144158. doi:10.1016/j.gene.2019.144158
Guimarães B.M., Prati C., Duarte M.A.H., Bramante C.M., Gandolfi M.G. Physicochemical properties of calcium silicate-based formulations MTA repair HP and MTA vitalcem. Journal of Applied Oral Science 2018; 26. doi:10.1590/1678-7757-2017-0115
Gandolfi M.G., Siboni F., Botero T., Bossù M., Riccitiello F., Prati C. Calcium silicate and calcium hydroxide materials for pulp capping: Biointeractivity, porosity, solubility and bioactivity of current formulations. J Appl Biomater Funct Mater 2015; 13 (1): 43-60. doi:10.5301/jabfm.5000201
Li X., Yoshihara K., De Munck J., Cokic S., Pongprueksa P., Putzeys E., et al. Modified tricalcium silicate cement formulations with added zirconium oxide. Clin Oral Investig 2017; 21 (3): 895-905. doi:10.1007/s00784-016-1843-y
Cardanho-Ramos C., Morais V.A. Mitochondrial biogenesis in neurons: How and where. Int J Mol Sci 2021; 22 (23): 13059. doi:10.3390/ijms222313059
Vergaças J.H.N., de Lima C.O., Barbosa A.F.A., Vieira V.T.L., dos Santos Antunes H., da Silva E.J.N.L. Marginal gaps and voids of three root-end filling materials: A microcomputed tomographic study. Microsc Res Tech 2022; 85 (2): 617-622. doi:10.1002/jemt.23935
Madurantakam P. Which is the superior retrograde filling material? We don’t know! Evid Based Dent 2022; 23 (2): 54-5. doi:10.1038/s41432-022-0237-z
dos Santos Costa F.M., Fernandes M.H., Batistuzzo de Medeiros S.R. Genotoxicity of root canal sealers: a literature review. Clin Oral Investig 2020; 24 (10): 3347-62. doi:10.1007/s00784-020-03478-z
Published
Issue
Section
License
Copyright (c) 2024 Verónica M. Méndez-González, Joselyn Martínez-López, Amaury Pozos-Guillén, Ana M. González-Amaro, Mariana Gutiérrez-Sánchez, Diana M. Escobar-García
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
ODOVTOS - Int. J. Dent. Sc. endorses CC BY-NC-SA
This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator. If you remix, adapt, or build upon the material, you must license the modified material under identical terms. CC BY-NC-SA includes the following elements:
BY: credit must be given to the creator.
NC: Only noncommercial uses of the work are permitted.
SA: Adaptations must be shared under the same terms.
