The Effect of Frankincense Extracts on Candida Albicans Adhesion and Surface Properties of Heat-Cured Acrylic Denture Base Resin

Authors

DOI:

https://doi.org/10.15517/kwbmz003

Keywords:

Polymethylmethacrylate; Denture base; Frankincense resin; Denture cleansers; Candida Albicans; Surface properties.

Abstract

Proper denture hygiene is one of the major predictors for the duration of rehabilitation treatment and the maintenance of oral mucosal health. Disinfectant solutions are often used as denture cleansers, yet their impact on acrylic resin-based dentures has been insufficiently investigated. This study aims to evaluate the effect of immersing heat cure acrylic denture base material in frankincense extract solution at different times intervals (24h and 72h) on the growth of Candida Albicans, micro-hardness and surface roughness. A total of ninety samples were prepared: 30 rectangular specimens (65×10×2.5 mm) intended for microhardness testing (n=30), and 60 disc-shaped specimens (10×2 mm) designated for antifungal activity and surface roughness measurements (n=30). For each test group, three further immersion periods were established: 10 samples per group (n=10). Sample size calculation was performed using G*Power. Data was first described and assessed for normal distribution, followed by parametric analyses with ANOVA and Tukey's HSD post hoc comparison and non-parametric analysis with the Kruskal–Wallis test followed by Dunn’s test. A significance level of < 0.05 was applied for all tests. In the result, there was an inhibition of Candida Albicans growth after immersion in frankincense extracts and a microhardness increase in the experimental groups compared to the control group regardless of immersion time. Additionally, the denture cleanser solution by frankincense extract also had a role in increasing surface roughness. To conclude, acrylic samples showed improved hardness and increased roughness which correlated with reduced fungal growth in all the specimens.

Downloads

Download data is not yet available.

References

Zhang X.-Y., Zhang X.-J., Huang Z.-L., Zhu B.-S., Chen R.-R. Hybrid effects of zirconia nanoparticles with aluminum borate whiskers on mechanical properties of denture base resin PMMA. Dent. Mater. J. 2014; 33 (1): 141-146. DOI: https://doi.org/10.4012/dmj.2013-054

Singh J., Dhiman R., Bedi R., Girish S. Flexible denture base material: A viable alternative to conventional acrylic denture base material. Contemp. Clin. Dent. 2011; 2 (4): 313-317. DOI: https://doi.org/10.4103/0976-237X.91795

Taher S.Y., Hussain W.A., editors. The effect of acidic treatment of carbon fiber on denture mechanical properties. J. Phys.: Conf. Ser. 2021: IOP Publishing.1879; 032082: 1-10. DOI: https://doi.org/10.1088/1742-6596/1879/3/032082

Anusavice K.J., Shen C., Rawls H.R. Phillips' science of dental materials: Elsevier Health Sciences; 2012.

Alhareb A.O., Ahmad Z.A. Effect of Al2O3/ZrO2 reinforcement on the mechanical properties of PMMA denture base. J. Reinf. Plast. Compos. 2011; 30 (1): 86-93. DOI: https://doi.org/10.1177/0731684410379511

Karkosh Z.S.A., Hussein B.M., AL-Wattar W.M.A. Effect of phosphoric containing and varnish-coated groups on Candida albicans adhesion and porosity of heat cure acrylic denture base material. Biomed. Pharmacol. J. 2018; 11 (1): 179-185. DOI: https://doi.org/10.13005/bpj/1360

Günther E., Kommerein N., Hahnel S. Biofilms on polymeric materials for the fabrication of removable dentures. Dtsch Zahnärztl Z. 2020; 2 (4): 142-151.

Fouda S.M., Gad M.M., Ellakany P., Al-Thobity A.M., Al-Harbi F.A., Virtanen J.I., Raustia A. The effect of nanodiamonds on candida albicans adhesion and surface characteristics of PMMA denture base material-an in vitro study. J. Appl. Oral Sci. 2019; 27: e20180779: 2-10. DOI: https://doi.org/10.1590/1678-7757-2018-0779

Coronado-Castellote L., Jiménez-Soriano Y. Clinical and microbiological diagnosis of oral candidiasis. J. Clin. Exp. Dent. 2013; 5 (5): e279-86. DOI: https://doi.org/10.4317/jced.51242

Hayran Y., Sarikaya I., Aydin A., Tekin Y.H. Determination of the effective anticandidal concentration of denture cleanser tablets on some denture base resins. J. Appl. Oral Sci. 2018; 26: e20170077:1-10. DOI: https://doi.org/10.1590/1678-7757-2017-0077

Ayaz E., Ustun S. Effect of staining and denture cleaning on color stability of differently polymerized denture base acrylic resins. Niger. J. Clin. Pract. 2020; 23 (3): 304-309. DOI: https://doi.org/10.4103/njcp.njcp_330_19

Huh J.-B., Lim Y., Youn H.-I., Chang B.M., Lee J.-Y., Shin S.-W. Effect of denture cleansers on Candida albicans biofilm formation over resilient liners. J. Adv. Prosthodont. 2014; 6 (2): 109-114. DOI: https://doi.org/10.4047/jap.2014.6.2.109

Echhpal U.R., Shah K.K., Ahmed N. Effectiveness of denture cleansers on candida albicans biofilm on conventionally fabricated, computer-aided design/computer-aided manufacturing-milled, and rapid-prototyped denture base resins: an in vitro study. Cureus. 2024; 16 (6): 1-10. DOI: https://doi.org/10.7759/cureus.63290

Mylonas P., Milward P., McAndrew R. Denture cleanliness and hygiene: an overview. Br. Dent. J. 2022; 233 (1): 20-26. DOI: https://doi.org/10.1038/s41415-022-4397-1

Al-Somaiday H., Al-Samaray M., Al-Samydai A. Role of herbal medicine in oral and dental health; ethnopharmacological study of medicinal plants in Iraq/Baghdad. Int J Res Pharm Sci. 2020; 11 (1): 1-8. DOI: https://doi.org/10.37506/v11/i1/2020/ijphrd/194105

Thulin M., Warfa A.M. The frankincense trees (Boswellia spp., Burseraceae) of northern Somalia and southern Arabia. Kew Bull. 1987; 42 (3): 487-500. DOI: https://doi.org/10.2307/4110063

Hasan N.N., Al-Hussainy R.H., Mohammed RS. Pharmaceutical and clinical perspectives on Boswellia resin as an adjunct in periodontal care. Rom. J. Pharm Pract. 2025; 18 (3-4): 89-94. DOI: https://doi.org/10.37897/RJPhP.2025.3-4.5

Bokelmann J. Medicinal herbs in primary care: Elsevier; 2021. DOI: https://doi.org/10.1016/B978-0-323-84676-9.00005-2

Yuan G., Wahlqvist M.L., He G., Yang M., Li D. Natural products and anti-inflammatory activity. Asia Pac. J. Clin. Nutr. 2006; 15 (2): 143-152.

Samani M.K., Mahmoodian H., Moghadamnia A., Mir A.P.B., Chitsazan M. The effect of Frankincense in the treatment of moderate plaque-induced gingivitis: a double blinded randomized clinical trial. Daru. 2011; 19 (4): 288-294.

Khoshbakht Z., Khashabi E., Torbati M., Lotfipour F., Hamishehkar H. Evaluation of herbal mouthwashes containing Zataria multiflora Boiss, frankincense and combination therapy on patients with gingivitis: A double-blind, randomized, controlled, clinical trial. Galen Med. J. 2019; 8: e1366: 1-10. DOI: https://doi.org/10.31661/gmj.v8i0.1366

Almohareb R.A., Barakat R.M., Eid E.E., Aldaws A., Alhagbani N., Almubayi R., Alsuwaid D., Algahtani F.N. Assessing the efficacy of frankincense extract as a root canal irrigant against Enterococcus faecalis. PlOS One. 2025; 20 (4): e0321458: 1-16. DOI: https://doi.org/10.1371/journal.pone.0321458

Yousef D.A., Eleknawy E., Arafa M.F., Negm W.A. The adjunctive effect of frankincense extract gel to nonsurgical treatment of chronic periodontitis: a randomized clinical study. Tanta Dent. J. 2024; 21 (3): 312-318. DOI: https://doi.org/10.4103/tdj.tdj_28_24

Biagi M.M., Rigillo G., Sarill M., Collotta D., Di Giacomo S., Di Sotto A., Grilli M., Luceri C., Milella L., Sangiovanni E. From preclinical to clinical evidence. Exploring the multiple perspectives and healing power of Boswellia serrata Roxb. ex Colebr. Pharmadva. 2023; 5 (1): 29-53. DOI: https://doi.org/10.36118/pharmadvances.2023.53

Almeida-da-Silva C.L.C., Sivakumar N., Asadi H., Chang-Chien A., Qoronfleh M.W., Ojcius D.M., Essa M.M. Effects of frankincense compounds on infection, inflammation, and oral health. Molecules. 2022; 27 (13): 4174: 1-19. DOI: https://doi.org/10.3390/molecules27134174

Bezerra I.M., Brito A.C.M., Cavalcanti Y.W., de Almeida LdFD. Inhibitory effect of phytochemicals on Candida albicans biofilms on denture liners surfaces. Res. Soc. Dev. 2020; 9 (10): 1-15.

Riyad Y.M., Barakat H.A., Amer S.A. Evaluation of the properties of frankincense powder and its water extracts and the effect of its addition on guava nectar characteristics. Egypt. J. Food Sci. 2020; 48 (1): 159-171.

Fatimah A.-O., Alharbi R.I., Albasher G., Almeer R., Alsaggabi N.S. Antifungal potential of aqueous extract of Boswellia carteri. J. Pure Appl. Microbiol. 2019; 13 (4): 2375-2381. DOI: https://doi.org/10.22207/JPAM.13.4.53

Fatalla A.A., Tukmachi M.S., Jani G.H., editors. Assessment of some mechanical properties of PMMA/silica/zirconia nanocomposite as a denture base material. IOP Conf. Ser: Mater. Sci. Eng., 2020; 987,012031: 1-10. DOI: https://doi.org/10.1088/1757-899X/987/1/012031

Kadhum F., AlKhayyat F., Jamal H. Inhibitory effect of chlorhexidine on biofilm formation by prosthetic surface pathogens: An in vitro study. BUMJ. 2026; 9 (1): 258-264. DOI: https://doi.org/10.38029/babcockuniv.med.j..v9i1.1342

Abd Kati F. Effects of chemical disinfectants on surface hardness of heat-cured acrylic resins. In vitro study. J. Oral Res. 2021;10 (6): 1-6. DOI: https://doi.org/10.17126/joralres.2021.074

Zhang K., Zhang S., Shi Y., Zhang L., Fu B. Effects of disinfectants on physical properties of denture base resins: A systematic review and meta-analysis. J. Prosthet. Dent. 2024; 131 (5): 841-858. DOI: https://doi.org/10.1016/j.prosdent.2022.03.020

Kaypetch R., Rudrakanjana P., Tua-Ngam P., Tosrisawatkasem O., Thairat S., Tonput P., Tantivitayakul P. Effects of two novel denture cleansers on multispecies microbial biofilms, stain removal and the denture surface: An in vitro study. BMC Oral Health. 2023; 23 (1): 1-12. DOI: https://doi.org/10.1186/s12903-023-03535-5

Abdel-Rahman H.K., Al-Sammaraie S.A.S. Effect of adding magnesium oxide nanoparticles on the antimicrobial activity of a denture soft liner. Polytech. J. 2020; 10 (2): 132-137. DOI: https://doi.org/10.25156/ptj.v10n2y2020.pp132-137

Gad M.M., Al-Thobity A.M., Shahin S.Y., Alsaqer B.T., Ali A.A. Inhibitory effect of zirconium oxide nanoparticles on Candida albicans adhesion to repaired polymethyl methacrylate denture bases and interim removable prostheses: a new approach for denture stomatitis prevention. Int. J. Nanomedicine. 2017; 12: 5409-5419. DOI: https://doi.org/10.2147/IJN.S142857

Abass E.F., Fatalla A.A., Matheel A.-R., Abdullah J.Y. Impact of Mouthwash Immersion on 3D-Printed Resin Material: A Comprehensive Evaluation of Surface Hardness, Surface Roughness, Wettability, and Characterization Using FTIR and FE-SEM Techniques. J Clin Exp Dent. 2026; 18 (2): 199-207. DOI: https://doi.org/10.4317/jced.63496

Porwal A., Khandelwal M., Punia V., Sharma V. Effect of denture cleansers on color stability, surface roughness, and hardness of different denture base resins. J. Indian Prosthodont. Soc. 2017; 17 (1): 61-67. DOI: https://doi.org/10.4103/0972-4052.197940

Mohammed A.M.E. Estimation of the active components in gum Arabic collected from western Sudan. Int J Sci Res. 2017; 6 (3): 1262-1282.

Jing Y., Nakajo S., Xia L., Nakaya K., Fang Q., Waxman S., Han R. Boswellic acid acetate induces differentiation and apoptosis in leukemia cell lines. Leuk. Res. 1999; 23 (1): 43-50. DOI: https://doi.org/10.1016/S0145-2126(98)00096-4

Mohamed A.A., Ali S.I., Kabiel H.F., Hegazy A.K., Kord M.A., EL-Baz F.K. Assessment of antioxidant and antimicrobial activities of essential oil and extracts of Boswellia carteri resin. Int. J. Pharmacogn. Phytochem. Res. 2015;7 (3): 502-509.

Hassan R.R.A., Mahmoud S.M.A., Karam Y.A., Salah S.M., Ebrahim S.Y., Abdelwahab M.A., Ahmed A.M.H., Ali H.M., Böhm M., and Salem M.Z.M. Application of frankincense and rice starch as eco-friendly substances for the resizing of paper as a conservation practice. BioResources. 2021:16 (4): 7180-7204. DOI: https://doi.org/10.15376/biores.16.4.7180-7204

Chandu G., Asnani P., Gupta S., Khan M.F. Comparative evaluation of effect of water absorption on the surface properties of heat cure acrylic: an in vitro study. J. Int. Oral Health (JIOH). 2015; 7 (4): 63-68.

Elrahim R.A.A., Shown A., Abdellah M., Abualsaud R. Helal MA. Impact of different chemical denture cleansers on the properties of digitally fabricated denture base resin materials. J. Prosthodont. 2024; 33 (7): 691-699. DOI: https://doi.org/10.1111/jopr.13761

Indriani L., Sitanya R.I., Fitri A. The effect of immersing the heat-cured acrylic resin plates into kitchen vinegar and star fruit (averrhoa bilimbi linn) solution to surface roughness base. J. Dentomaxillofac. Sci. 2023; 8 (1): 1-3. DOI: https://doi.org/10.15562/jdmfs.v8i1.1419

Published

2026-09-21