Remineralizing Effect of Xilitol, Juniperus Communis and Camellia Sinensis Added to a Toothpaste: An In Vitro Study

Authors

  • Sandra I. Jiménez-Gayosso DDS Advanced Studies and Research Centre in Dentistry "Dr. Keisaburo Miyata" of Faculty of Dentistry at Autonomous University State of Mexico, Toluca, Mexico Academic Area of Dentistry of Health Sciences Institute at Autonomous University of Hidalgo State, Pachuca, Mexico Author
  • Edith Lara-Carrillo DDS, MSc, PhD Advanced Studies and Research Centre in Dentistry "Dr. Keisaburo Miyata" of Faculty of Dentistry at Autonomous University State of Mexico, Toluca, Mexico Author
  • Rogelio J. Scougall-Vilchis DDS, MSc, PhD Advanced Studies and Research Centre in Dentistry "Dr. Keisaburo Miyata" of Faculty of Dentistry at Autonomous University State of Mexico, Toluca, Mexico Author
  • Raúl A. Morales-Luckie BS Chem, PhD Sustainable Chemistry UAEMex-UNAM Research Center of Faculty of Chemistry at Autonomous University State of Mexico, Toluca, Mexico Author
  • Carlo E. Medina Solís DDS, MSc Advanced Studies and Research Centre in Dentistry "Dr. Keisaburo Miyata" of Faculty of Dentistry at Autonomous University State of Mexico, Toluca, Mexico. Academic Area of Dentistry of Health Sciences Institute at Autonomous University of Hidalgo State, Pachuca, Mexico Author
  • Ulises Velázquez-Enríquez DDS, MSc, PhD Advanced Studies and Research Centre in Dentistry "Dr. Keisaburo Miyata" of Faculty of Dentistry at Autonomous University State of Mexico, Toluca, Mexico Author
  • Gerardo Maupomé DDS, MsC, PhD Richard M. Fairbanks School of Public Health, Indiana University/Purdue University in Indianapolis. Indianapolis, USA. Indiana University Network Science Institute. Bloomington, USA Author
  • Brenda Herrera-Serna DDS, MSc Advanced Studies and Research Centre in Dentistry "Dr. Keisaburo Miyata" of Faculty of Dentistry at Autonomous University State of Mexico, Toluca, Mexico Autonomous University of Manizales, Colombia Author

DOI:

https://doi.org/10.15517/ijds.v0i0.34573

Keywords:

Remineralization, Xylitol, Juniperus Communis, Camellia Sinensis, Toothpaste, In Vitro Techniques

Abstract

The aim of this study was to explore the remineralizing effect of toothpastes based on Xilitol, Camellia Sinensis and Juniperus Communis. An in vitro experimental study was carried out in an 18 human premolars sample, which were treated with one of the 3 evaluated toothpastes and a control fluoride one. The atomic percentages of Ca and P were evaluated by Energy Dispersive X-ray Spectroscopy (EDS). In addition, the enamel surface of treated teeth was visualized by Scanning Electron Microscopy (SEM). The evaluations were carried out in three stages: pre-treatment, after an artificial demineralization process and after the treatment with the toothpastes. In the statistical analysis, the one-way ANOVA and Pearson correlation tests were used. Xilitol and Juniperus Communis showed the greatest gain of P ions compared to the control group (p<0.01). As for Ca, the group treated with xylitol-based toothpaste showed more ion gain compared with the control group (p<0.01). In the Pearson correlation test between Ca and P, statistically significant correlations were observed in all groups (p<0.01), ranging between r=0.7413 (Xylitol Group) and r=0.9510 (Control Group). We concluded that Xylitol paste showed the highest remineralizing property, both in the EDS analysis and in the SEM images.

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Published

2026-06-15