Remineralizing Effect of Xilitol, Juniperus Communis and Camellia Sinensis Added to a Toothpaste: An In Vitro Study
DOI:
https://doi.org/10.15517/ijds.v0i0.34573Keywords:
Remineralization, Xylitol, Juniperus Communis, Camellia Sinensis, Toothpaste, In Vitro TechniquesAbstract
The aim of this study was to explore the remineralizing effect of toothpastes based on Xilitol, Camellia Sinensis and Juniperus Communis. An in vitro experimental study was carried out in an 18 human premolars sample, which were treated with one of the 3 evaluated toothpastes and a control fluoride one. The atomic percentages of Ca and P were evaluated by Energy Dispersive X-ray Spectroscopy (EDS). In addition, the enamel surface of treated teeth was visualized by Scanning Electron Microscopy (SEM). The evaluations were carried out in three stages: pre-treatment, after an artificial demineralization process and after the treatment with the toothpastes. In the statistical analysis, the one-way ANOVA and Pearson correlation tests were used. Xilitol and Juniperus Communis showed the greatest gain of P ions compared to the control group (p<0.01). As for Ca, the group treated with xylitol-based toothpaste showed more ion gain compared with the control group (p<0.01). In the Pearson correlation test between Ca and P, statistically significant correlations were observed in all groups (p<0.01), ranging between r=0.7413 (Xylitol Group) and r=0.9510 (Control Group). We concluded that Xylitol paste showed the highest remineralizing property, both in the EDS analysis and in the SEM images.
Downloads
References
Abdulraheam R. H., Garib B. T. Effect of different tea in remineralization of artificially-induced initial enamel caries of human teeth (Study in vitro). J Dent Sci (Tikrit) 2011; 1:19-24. DOI: https://doi.org/10.25130/tjds.1.1.4
Ando M., Ferreira-Zandoná A. G., Eckert G. J., Zero D. T., Stookey G. K. Pilot clinical study to assess caries lesion activity using quantitative light-induced fluorescence during dehydration. Journal of Biomedical Optics 2017; 22: 03: 50-55. DOI: https://doi.org/10.1117/1.JBO.22.3.035005
Anita P., Sivasamy S., Madam-Kumar P. D., Balan I. N., Ethiraj S. In vitro antibacterial activity of Camellia sinensis extract against cariogenic microorganisms. J Basic Clin Pharm 2014; 6: 35-39. DOI: https://doi.org/10.4103/0976-0105.145777
Arends J., Christoffersen J., Schuthof J., and Smits M. T. Influence of xylitol on demineralization of enamel. Caries Res 1984; 18: 296-301. DOI: https://doi.org/10.1159/000260780
Azzimonti B., Cochis A., El Beyrouthy M., Iriti M, Uberti F, Sorrentino R, et al. Essential oil from berries of lebanese Juniperus excelsa M. Bieb displays similar antibacterial activity to chlorhexidine but higher cytocompatibility with human oral primary cells. Molecules 2015; 20: 9344-9357. DOI: https://doi.org/10.3390/molecules20059344
Barbosa C. S., Kato M. T., Buzalaf M. A. R. Effect of supplementation of soft drinks with green tea extract on their erosive potential against dentine. Aust Dent J 2011; 56: 317-321. DOI: https://doi.org/10.1111/j.1834-7819.2011.01338.x
Cardoso C. A., Cassiano L. P., Costa E. N., Souza-E-Silva C. M., Magalhães AC, Grizzo LT, et al. Effect of xylitol varnishes on remineralization of artificial enamel caries lesions in situ. J Dent 2016b; 50: 74-78. DOI: https://doi.org/10.1016/j.jdent.2016.03.011
Cardoso J. G., Iorio N. L., Rodrigues L. F., Couri M. L., Farah A., Maia L. C., et al. Influence of a Brazilian wild green propolis on the enamel mineral loss and Streptococcus mutans’ count in dental biofilm. Arch Oral Biol 2016a; 65: 77-81. DOI: https://doi.org/10.1016/j.archoralbio.2016.02.001
Castellanos J. E., Marín L. M., Úsuga M. V., Castiblanco G. A., Martignon S. La remineralización del esmalte bajo el entendimiento actual de la caries dental. Univ Odontol 2013; 32: 49-59.
Cheng L., Li J., He L., Zhou X. Natural products and caries prevention. Caries Res 2015; 49: 38-45. DOI: https://doi.org/10.1159/000377734
Cochrane N. J., Cai F., Huq N. L., Burrow M. F., Reynolds E. C. New approaches to enhanced remineralization of tooth enamel. J Dent Res 2010; 89: 1187-1197. DOI: https://doi.org/10.1177/0022034510376046
Donahue G. J., Waddell N., Plough A. L., del Aguila M. A., Garland T. E. The ABCDs of treating the most prevalent childhood disease. Am J Public Health 2005; 95: 1322-1324. DOI: https://doi.org/10.2105/AJPH.2004.057778
Ekambaram M., Itthagarun A., King N. M. Comparison of the remineralizing potential of child formula dentifrices. Int J Paediatr Dent 2011; 21: 132-140. DOI: https://doi.org/10.1111/j.1365-263X.2010.01101.x
Featherstone J. D. The science and practice of caries prevention. J Am Dent Assoc 2000; 131: 887-899. DOI: https://doi.org/10.14219/jada.archive.2000.0307
Grewal N., Gumber S., Kaur N. Comparative evaluation of enamel remineralization potential of processed cheese, calcium phosphate-based syntetic agent, and a fluoride-containing toothpaste: An in situ study. J Indian Soc Pedod Prev Dent 2017; 35: 19-27. DOI: https://doi.org/10.4103/0970-4388.199222
Hariri I., Sadr A., Nakashima S., Shimada Y., Tagami J., Sumi Y. Estimation of the enamel and dentin mineral content from the refractive index. Caries Res 2013; 47: 18-26. DOI: https://doi.org/10.1159/000342416
Höferl M., Stoilova I., Schmidt E., Wanner J., Jirovetz L., Trifinova D., et al. Chemical composition and antioxidant properties of Juniper Berry (Juniperus communis L.) essential oil. Action of the essential oil on the antioxidant protection of Saccharomyces cerevisiae model organism. Antioxidants (Basel) 2014; 3: 81-98. DOI: https://doi.org/10.3390/antiox3010081
Innes N. P., Frencken J. E., Bjorndal L., Maltz M., Manton D. J., Ricketts D., et al. Managing carious lesions: Consensus recommendations on terminology. Adv Dent Res 2016; 28: 49-57. DOI: https://doi.org/10.1177/0022034516639276
Khurshid Z., Zafar M. S., Zohaib S., Najeeb S., Naseem M. Green tea (Camellia sinensis): Chemistry and oral health. Open Dent J 2016; 10: 166-173. DOI: https://doi.org/10.2174/1874210601610010166
Longbottom C. L., Huysmans M. C., Pitts N. B., Fontana, M. Glossary of key terms. Monogr Oral Sci. 2009; 21: 209-216. DOI: https://doi.org/10.1159/000224225
Mäkinen K. K., Isotupa P. K., Kivilompolo T., Makinen L. P., Tiovanen J., Soderling E. Comparison of erythritol and xylitol saliva stimulants in the control of dental plaque and mutans streptococci. Caries Res 2001; 35: 129-135. DOI: https://doi.org/10.1159/000047444
Malekafzali B., Ekrami M., Mirfasihi A., Abdolazimi Z. Remineralizing Effect of Child Formula Dentifrices on Artificial Enamel Caries Using a pH Cycling Model. J Dent (Tehran) 2015; 12 (1): 11-17.
Martorell P., Forment J. V., de Llanos R., Montón F., Llopis S., González N., Genovés S., Cienfuegos E., Monzó H., Ramón D. Uso de Saccharomyces cerevisiae y Caenorhabditis elegans como modelo organismos para estudiar el efecto de los polifenoles del cacao en la resistencia al estrés oxidativo. J. Agric. Comida Chem. 2011; 59: 2077-2085. DOI: https://doi.org/10.1021/jf104217g
Masood M., Yusof N., Hassan M. I., Jaafar N. Longitudinal study of dental caries increment in Malaysian school children: a 5-year cohort study. Asia Pac J Public Health 2014; 26: 260-267. DOI: https://doi.org/10.1177/1010539511420704
Miake Y., Saeki Y., Takahashi M., Yanagisawa T. Remineralization effects of xylitol on demineralized enamel. J Electron Microsc 2003; 52 (5): 471-476. DOI: https://doi.org/10.1093/jmicro/52.5.471
Mirkarimi M., Toomarian L. Effect of green tea extract on the treatment of dentin erosion: an in vitro study. J Dent (Tehran) 2012; 9: 224-228.
Peres M. A., Barros A. J., Peres K. G., Araujo C. L., Menezes A. M. Life course dental caries determinants and predictors in children aged 12 years: a population-based birth cohort. Community Dent Oral Epidemiol 2009; 37: 123-133. DOI: https://doi.org/10.1111/j.1600-0528.2009.00460.x
Rochel I. D., Souza J. G., Silva T. C., Pereira A. F. F., Rios D., Buzalaf M. A. R., et al. Effect of experimental xilitol and fluoride containing dentifrices on enamel erosion with or without abrasion in vitro. J Oral Sci 2011; 53: 163-168. DOI: https://doi.org/10.2334/josnusd.53.163
Spratt D. A., Daglia M., Papetti A., Stauder M., O’Donell D., Ciric L., et al. Evaluation of plant and fungal extracts for their potential antigingivitis and anticaries activity. J Biomed Biotechnol 2012; 2012: About 12 pages. DOI: https://doi.org/10.1155/2012/510198
Ten Cate J. M., Arends J. Remineralization of artificial enamel lesions in vitro: III. A study of the deposition mechanism. Caries Res 1980; 14 (6): 351-358. DOI: https://doi.org/10.1159/000260477
Published
Issue
Section
License
Copyright (c) 2018 Odovtos - International Journal of Dental Sciences

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
ODOVTOS - Int. J. Dent. Sc. endorses CC BY-NC-SA
This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator. If you remix, adapt, or build upon the material, you must license the modified material under identical terms. CC BY-NC-SA includes the following elements:
BY: credit must be given to the creator.
NC: Only noncommercial uses of the work are permitted.
SA: Adaptations must be shared under the same terms.





