Knowledge status of predators of the freshwater prawn Cryphiops caementarius (Decapoda: Palaemonidae) in river systems along the North Western Andean region from Perú and Chile

Introduction: Cryphiops caementarius, is an endemic prawn from river ecosystems in Southern Perú and Northern Chile. In these watersheds, natural populations are threatened by multiple anthropic interventions such as mining activities and fishing pressure, but also, by increasing impacts of invasive exotic predators. Information related with predation on C. caementarius is scarce representing a major knowledge gap to ensure suitable management and conservation practices. Accordingly, the further deepening in relevant aspects such as the estimation of populations losses due to predators has been indicated as critical by relevant agencies as FAO. Objective: To conduct a complete registry and classification of C. caementarius predators, updating the knowledge for the Andean region. Methods: A thorough bibliographic review on existing information on C. caementarius’ predators from Perú and Chile was carried out calculating percentage of occurrence, relative frequency of species, zoogeographic origin, trophic structure, phenotypic attributes, river zone that inhabits and life stage of incidence. The registry was complemented using semi-structured interviews from prawn ́s fishers in central-Northern Chile. Results: C. caementarius is predated along the entire fluvial network (estuary, potamon and rhithron) by 21 species of opportunists, carnivores, omnivores and herbivores, mostly aquatic vertebrates. In turn, these belong to 15 families and six guilds of native, exotic and cosmopolitan species. Approximately 86 % predate on adult prawns, whilst, 71 % on juveniles and 24 % on larvae. More than 40 % of the predation pressure seems to be exerted by native waterfowls and 24 % by exotic fish. Conclusions: The results emphasize the relevance for conservation of native species of predators in the Andean region and the need of control measures on exotic species in a latitudinal basis. Accordingly, this review may serve as an initial decision-making tool for future conservation efforts both on prawns and native biota, but also supporting restocking actions, fisheries management plans and risk assessment for C. caementarius populations across the Andean region.

However, these factors do not exclusively affect C. caementarius, but most of the species of freshwater prawns worldwide (e.g. Bowles, Aziz, & Knight, 2000;Snyder, Anderson, & Pringle, 2011). For this reason, the Food and Agriculture Organization of the United Nations (FAO; http://www.fao.org) indicates the need to further deepen in relevant aspects of these organisms such as: a) the ethology of juveniles, b) infectiology and parasitology, and c) the quantitative estimation of populations losses due to predation. Accordingly, information related with key ecological interactions such as predation is scarce representing a major knowledge gap to guarantee correct management and conservation practices (Parsons, 2005).
Cryphiops caementarius is one of the most studied prawns across the Andean region (Jara, Rudolph, & González, 2006) with extensive reviews on natural history, fishing and cultivation (e.g. Bahamonde & Vila, 1971;Morales & Meruane, 2013 a, b). To date, 69 articles and technical reports have been published on C. caementarius in Perú and Chile. From these, only 23 are currently indexed on SCOPUS (IFOP, 2019). These articles include eight subjects: a) aquaculture and physiology (N = 25; 36.3 %), b) fishery (N = 16; 23.2 %), c) biology and morphology (N = 10; 14.5 %), d), ecology (N = 4; 5.8 %), e) diet (N = 3; 4.3 %), f) systematics and phylogeny (N = 2; 2.9 %), g) economic studies (N = 2; 2.9 %), and h) others (N = 7; 10.1 %). These figures suggest that relevant aspects indicated by FAO (e.g. ecology) are poorly documented. Hence, the main objective of this work is to conduct a bibliographic review of the existing background information on C. caementarius' predators in the North Western watersheds of the Andean region of Perú and Chile. Also, this register was complemented with semi-structured interviews from prawn´s fishers. Then, the state of knowledge of this species is discussed in function of potential ecological and trophic implications of predator's species on the populations of C. caementarius emphasizing the relevance of conservation of the diversity of native predators in the Andean region.
Bibliographic revision and semi-structured interviews: The collection of information was carried out through an exhaustive review of scientific journals, academic thesis, government and academic web platforms. A total of nine studies were collected from Perú (42.9 %) and 12 from Chile (57.1 %), covering the last 62 years (1958-2019) (Digital Appendix 1). In addition, semi-structured interviews were performed during March 2019 in two commercial prawn fisher organizations (N = 17) from the rivers Illapel and Choapa (ca. 31°S; central-Northern Chile). The age group of interviewees varied between 29-65 years old and included four women (23.5 %) and 13 men (76.5 %). The interviewees answered three questions: a) what animals have you observed feeding on prawns? b) have you ever observed prawns in the stomach of some animals? Then, which ones? and c) have you ever observed cannibalism among prawns? If so, can you explain under what circumstances?
Ontogenic stages were classified according to cephalothorax length into larvae (since ca. 0.4 mm), juveniles (> 2.5 mm) and adults (ca. 22.0 mm) (Morales, Meruane, Galleguillos, Rivera, & Hosokawa, 2006). Due to the lack of clarity about the identity of fish of the genus Basilichthys and Odonthestes in the literature, these were referred generically as silversides. The proportion of species was determined through percentage of occurrence frequency (OF) for each species in the total number of rivers and relative frequency (RF), which corresponds to RF= (fi/∑fi) x 100, where fi = number of times a species was registered and ∑fi = the total number of records of all species.

Semi-structured interviews:
The group interviews yielded the following results: a) the main predators of C. caementarius at Illapel and Choapa river included five species of waterfowls (A. alba, the cocoi heron Ardea cocoi (Linnaeus, 1766), E. thula, N. nycticorax and P. brasilianus), two fish (the common carp Cyprinus carpio (Linnaeus, 1758) and O. mykiss), and the Chilean giant frog Calyptocephalella gayi (Duméril & Bibron, 1841) ( Fig.  3), b). All interviewees declared to have found remains of prawns in the stomach content of the locally consumed frog C. gayi, c) all interviewees agreed that the river prawn is a species that showed cannibalism, in particular among individuals that happen to use the same lair.

DISCUSSION
Cryphiops caementarius encompasses an important food item for a wide range of taxa as well as a source of livelihood for commercial fishers. In nature, the presence of predators extends throughout all zones of the river, but they are particularly abundant (> 90 %) in estuaries, probably due to the greater productivity of these biomes. More than 40 % of the total predation pressure seems to be exerted by native waterfowls. Interestingly, an important fraction (23.8 %) is potentially carried out by exotic fish (e.g. salmonids). This predators     (Rudolph, 2013). In addition, similarly to other species of palaemonid prawns when faced with food shortage, C. caementarius adopts cannibalistic behaviour. However, this study suggests that this effect may be minor compared with other predators. Nevertheless, cannibalism in C. caementarius have been confirmed by the narrative of commercial fishers in Illapel and Choapa rivers, and has also, been observed under experimental laboratory conditions (Morales & Meruane, 2013 b).
It is important to highlight that exotic fish predators such as O. mykiss, the brown trout Salmo trutta (Linnaeus, 1758), C. carpio and G. affinis, have been identified within the list of the 100 world's worst invasive alien species by the International Union for Conservation of Nature (IUCN; http://www.iucn.org). Currently, these species are all present in inland waters from Perú and Chile, along with A. facetus and the African frog Xenopus laevis (Daudin, 1802), which have been listed by the Chilean Ministry of Environment (MMA) as exotic species of major concern (https://www. especies-exoticas.mma.gob.cl).
The impact of exotic predatory species on native fauna goes further predation. Sublethal effects, usually of unknown extent on the trophic interactions and habitat displacement have been documented in earlier studies. Among these predators, the fish as A. facetus is the only species that have evidenced until 50 % of C. caementarius in stomach content (Áviles, López, & Flores, 2018). On the contrary, there's no direct evidence of predation on C. caementarius by G. affinis, but this species is a well-known active predator of planktonic microcrustaceans. However, there is evidence that this species competes directly for food and shelter with prawns, such as Palaemon antennarius (H. Milne Edwards, 1837) and Atyaephyra thyamisensis (Christodoulou, Antoniou, Magoulas, & Koukouras, 2012) (Gkenas, Oikonomou, Economou, Kiosse, & Leonardos, 2012). On the other hand, X. laevis, O. mykiss and S. trutta are notable predators of benthic macroinvertebrates, including crustaceans (Encina, Vega, Lara, & Ríos-Escalante, 2017;Charrier, 2019). However, so far, studies on these predators have not detected C. caementarius as a food item in the diet of these fish, but this may be due that study areas were outside the habitat range of C. caementarius. On the other hand, the recent documented expansion of X. laevis into river systems of central-North Chile (Mora et al., 2019), suggests emergent effects on C. caementarius populations comparable to those exerted by the salmonids on the crayfish S. spinifrons (Encina Vega, Lara, & Ríos-Escalante, 2017), a species with similar habits of those observed in C. caementarius. The uncontrolled introduction of exotic species corresponds to the second cause of extinction after habitat fragmentation, and therefore, a major threat to world´s biodiversity (Doherty, Glen, Nimmo, Ritchie, & Dickman, 2016). Accordingly, the introduction and subsequent geographic expansion of exotic predators appears as a major risk for C. caementarius. In addition, this may have important implications for commercial fisher´s livelihood from the Andean region of Perú and Chile. In this context, the ecological role of native predators, such as herons, cormorants and frogs in controlling exotic species in the Andean region such as the salmonids (Alarcón, Macchi, Trejo, & Alonso, 2012), C. carpio (Pérez, De Lope, Turégano, & De la Cruz, 1991), G. affinis (Ashoori, Varasteh-Moradi, Reza-Rezaiee, & Salman-Mahiny, 2017) and the frog X. laevis (Mora, Constanzo-Chávez, Contardo, & Labra, 2016;Charrier, 2019), emerges as of utmost relevance. This have been suggested by Hammerschlag et al. (2019) as one of the many ecosystem functions/services provided by native predators in an ever-changing environment. However, some native predators as the frog C. gayi are in high risk, because it is target of illegal fishing pressure since is considered a culinary delight (Charrier, 2019). This suggests, that furtive fishers may facilitate the prevalence of exotic species by eliminating native predators and competitors. The background information provided in this study stresses out the need of enforcing conservation measures for the protection of native predators in particular, those from ecosystems subjected to high anthropic perturbations as river basins.
From a fisheries perspective, the extraction of C. caementarius in Chile is carried out under an open-access regime but the activity has not been legally recognized by the National Fisheries Authority. Basic aspects of fisheries as the up-to-date knowledge of landings are still incomplete at least in Chile (Digital Appendix 4). Nevertheless, from 1986 several regulations were proposed including a summer-mid autumn harvest ban, a permanent ban on ovigerous females and a minimum harvest size (30 mm cephalothorax length). However, enforcement and compliance on these regulations are unknown. On the other hand, C. caementarius have been classified as of Least Concern species, due to lack of basic information and inconsistent updating of population dynamics (IFOP, 2019). Fortunately, since 2013 the species has been categorized as Vulnerable by the MMA (https://www.clasificacionespecies.mma.gob. cl), and the IUCN, which has included C. caementarius in the red list of threatened species (https://www.iucnredlist.org). Currently, natural populations in Northern Chile are facing the worst drought in recent years (https://www.cr2. cl/megasequia). This have been accentuated by water shortage mitigating measures promoted by Chilean government through direct physical alteration of rivers. Furthermore, a recent study has reported a drastic reduction in the original spatial distribution of C. caementarius in the Choapa River (IFOP, 2019). Accordingly, as an amphidromous species is highly susceptible to habitat modification and connectivity disruptions across the basins (Morales & Meruane, 2013 a).
The information gathered in this review can be useful for enabling the risk assessment of C. caementarius populations in river systems of the Andean region illustrating the exotic predator´s guilds that may threaten prawns' populations. Likewise, it can act as supporting information for selection criteria for conservation or restocking initiatives, and the implementation of extinction risk assessment, a tool widely used for species inhabiting altered environments. This review has shown that C. caementarius seems to support a wide range of predators from the Andean region, however, these includes fast-spreading exotic species. The later may be detrimental for natural populations along the entire watersheds inhabited by the species. In turn, this may have further social-ecological implications for ecosystems and people since the most of basins are subjected to additional anthropogenic uses. The lack of information and the complexity of the multiple interactions with predators suggests a risk scenario for C. caementarius. The present work can underpin and guide either conservation and management programs able to support three main axes for socio-ecological development: (1) suitable watershed administration, (2) fisheries recognition towards conservation and (3) improved control of introduced-invasive species. At this regard, implementation of hatchery reared juveniles and fully implemented restocking programs could be established along with protection of the waterways, at the least, as a precautionary principle. This will be achieved in a first stage only after official governmental recognition of C. caementarius as an endangered fishing resource that support both natural heritage and social-ecological systems from the Andean region.
Ethical statement: authors declare that they all agree with this publication and made significant contributions; that there is no conflict of interest of any kind; and that we followed all pertinent ethical and legal procedures and requirements. All financial sources are fully and clearly stated in the acknowledgements section. A signed document has been filed in the journal archives.

ACKNOWLEDGMENTS
This work was supported by the Grant "Consolidation program of the Aquaculture Fisheries Strategy (AFS) of the freshwater prawn Cryphiops caementarius in the basin of the Choapa River" funded for Regional Government of Coquimbo, Chile (Code BIP: 30480241-0).