Revista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

OAI: https://revistas.ucr.ac.cr/index.php/rbt/oai
In vitro micropropagation of <i>Lawsonia inermis</i>(Lythraceae)
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Keywords

in vitro
micropropagation
medicinal plants
shoot multiplication

How to Cite

Rout, G. R., Das, G., Samantaray, S., & Das, P. (2001). In vitro micropropagation of <i>Lawsonia inermis</i>(Lythraceae). Revista De Biología Tropical, 49(3-4), 957–963. Retrieved from https://revistas.ucr.ac.cr/index.php/rbt/article/view/18043

Abstract

A successful protocol was developed for mass propagation of Lawsonia inermis Linn., an important medicinal plant. Multiple shoots were induced in apical and axillary meristems derived from mature explants of L. inermis on Murashige and Skoog (1962) medium supplemented with 0.25 mg/l 6-benzylaminopurine (BA), 0.25 mg/l Kinetin (Kn), 0.5 mg/l ascorbic acid and 3 % (w/v) sucrose. The rate of multiplication was higher when the cultures were incubated under continuous light rather than the 14 hr photoperiod. Rooting was readily achieved upon transferring the microshoots onto MS basal semi-solid medium supplemented with 0.25 mg/l indole-3-butyric acid (IBA) after ten days of culture. Micropropagated plantlets were acclimatized and successfully grown in soil.
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