Abstract
Along the Mexican coast, harmful algae blooms (HAB) have become more frequent, and therefore, there is an urgent need to establish monitoring programs to avoid the undesired consequences of HAB in human and natural ecosystems. In this work, we analyzed the pigment signatures and the species composition from phytoplankton samples to evaluate the utility of the specific pigment “fingerprints” in HAB monitoring programs. Vertical profiles from a coastal lagoon and temporal samples of a red tide occurring in a shrimp-culture pond and in a coastal zone were taken into consideration. Between 76% and 84% of dinoflagellate and diatom cell density was explained by their specific signature variation, in both vertical and temporal samples. Only the variation of zeaxanthin and the cyanobacteria Anabaena sp. showed a poor relationship, probably from difficulties in counting other cyanobacteria present in the samples examined with the microscopic method. These results suggest that inclusion of pigment analysis in the study and monitoring programs dealing with harmful algae would be very usefulReferences
Barlow, R.G., R.F.C. Mantoura, M.A. Gough & T.W. Fileman. 1993. Pigment signatures of the phytoplankton composition in the Northeastern Atlantic during the 1990 spring bloom. Deep-Sea Res. 40: 459-477.
Blasco, D. 1977. Red tide in the upwelling region of Baja California. Limnol. Oceanogr. 22: 255-263.
Bustillos-Guzmán, J., H. Claustre & J.C. Marty. 1995. Specific phytoplankton signatures and their relationship to hydrographic conditions in the coastal northwestern Mediterranean Sea. Mar. Ecol. Prog. Ser. 124: 247-258.
Bustillos-Guzmán, J. & J. Diogene. 1998. Pigment content and toxicity in three strains of Gambierdiscus toxicus: implications of cell volume differences, pp. 372-373. In B. Reguera, J. Blanco, M.L. Fernández & T. Wyatt (eds.). Harmful Algae. Xunta de Galicia and IOC-UNESCO, Spain.
Bustillos-Guzmán, J., D. López-Cortés, F. Hernández & I. Murillo. 2000. Pigment signatures associated with a coastal anoxic lagoon: Bahía Concepción, Gulf of California. J. Exp. Mar. Biol. Ecol. 249: 77-88.
Claustre, H., P. Kerherve, J.C. Marty, L. Prieur & J.H. Hecq. 1994. Phytoplankton distribution associated with a geostrophic front: ecological and biogeochemical implications. J. Mar. Res. 52: 711-742.
Cortés-Altamirano, R. 1984. Mareas rojas producidas por el ciliado Mesodinium rubrum (Lohman) en el litoral de Mazatlán, Sinaloa, México. Biotica 9: 259-269.
Cortés-Altamirano, R., L. Muñoz-Cabrera & O. Sotomayor-Navarro. 1993. Envenenamiento paralítico por mariscos (PSP), causado por Pyrodinium bahamense var. compressum en la costa suroeste de México. An. Inst. Cienc. Mar Limnol. 20: 43-54.
Cortés-Altamirano, R., D.U. Hernandez-Becerril & R. Luna-Soria. 1996. Red tides in México: A review, pp. 101-105. In T. Yasumoto, Y. Oshima & Y. Fukuyo (eds.). Harmful and Toxic Algal Blooms. Intergovernmental Oceanographic Commission of UNESCO, Sendai, Japan.
Cortés-Altamirano, R. & R. Alonso-Rodríguez. 1997. Mareas rojas durante 1997 en la Bahía de Mazatlán, Sinaloa, México. Cien. Mar UAS 15: 31-37.
Falkowski, P. & J. LaRoche. 1991. Acclimation to spectral irradiance in algae. J. Phycol. 27: 8-14.
Gárate-Lizárraga, I., J.J. Bustillos-Guzmán, L.M. Morquecho & C.H. Lechuga-Deveze. 2000. First Outbreak of Cochlodinium polykrikoides in the Gulf of California. Harmful Algae News 21: 7.
Gárate-Lizárraga, I., M.L. Hernández-Orozco, C. BandSchmidt & G. Serrano-Casillas. 2001a. Red tides along the coasts of Baja California Sur, México (1984 to 2001). Oceánides 16: 127-134.
Gárate-Lizárraga, I., D. López-Cortés, J. BustillosGuzmán, F. Hernández-Sandoval & I. MurilloMurillo. 2001b. El Niño 1997/1998: Impacto de la biomasa de dinoflagelados en Bahía Concepción, Golfo de California, pp. 153-162. In E. Escobar, M. Bonilla, A. Badan, M. Caballero & A. Winckell (eds.). Los efectos del evento del fenómeno El Niño en México, 1997-1998. CONACyT, México.
Gárate-Lizárraga, I., D. López-Cortés, J. BustillosGuzmán, F. Hernández-Sandoval & I. MurilloMurillo. 2002a. Physicochemical characteristics and phytoplankton biomass during El Niño 1997-1998 in Bahía Concepción, Gulf of California. Mem. Symposium “North Pacific Transitional Areas”. La Paz, B.C.S. México.
Gárate-Lizárraga, I., C.J. Band-Schmidt, R. CervantesDuarte & D. Escobedo-Urias. 2002b. Mareas rojas de Mesodinium rubrum (Lohmann) Hamburger and Budenbrock ocurridas en el Golfo de California, (Invierno de 1998). Hidrobiologia 16: 310-317.
Goericke, R. & D.J. Repeta. 1993. Chlorophylls a and b and divinyl-chlorophylls a and b in the open subtropical North Atlantic Ocean. Mar. Ecol. Progr. Ser. 101: 307-313.
Graham, H.W. 1943. Gymnodinium catenatum, a new dinoflagellate from the Gulf of California. Trans. Am. Microsc. Soc. 62: 259-261.
Hasle, G.R. 1978. Using the inverted microscope, pp. 191196. In A. Sournia (ed.). Phytoplankton Manual. UNESCO.
Heredia-Tapia, A., B.O. Arredondo-Vega, E. NuñezVázquez & J.L. Ochoa. 2000. Partial biochemical characterization and toxicological evaluation of the dinoflagellate Exuviaella lima isolated from El Pardito Island in Baja California Sur, México. Mem. 9th International Conference on Algal Blooms. Tasmania.
Hernández-Becerril, D.U. 1998. Species of the planktonic diatom genus Pseudo-nitzschia of the Pacific coasts of Mexico. Hidrobiologia 379: 77-84.
Jeffrey, S.W. 1961. Paper-chromatography separation of chlorophylls and carotenoids from marine algae. Biochem. J. 80: 336-342.
Jeffrey, S.W. 1968. Quantitative thin-layer chromatography of chlorophylls and carotenoids from marine algae. Bioch. Biophy. Acta 162: 271-285.
Jeffrey, S.W., M. Sielicki & F.T. Haxo. 1975. Chloroplast pigments patterns in dinoflagellates. J. Phycol. 11: 374-384.
Jeffrey, S.W. & M. Vesk. 1997. Introduction to marine phytoplankton and their pigment signatures, pp. 3784. In S.W. Jeffrey, R.F.C. Mantoura & S.W. Wright (eds.). Phytoplankton pigments in oceanography. Monogr. Oceanogr. Method. 10. UNESCO.
Johansen, J.E., W.A. Svec, S. Liaaen-Jensen & F.T. Haxo. 1974. Carotenoids of the dinophyceae. Phytoch. 13: 2261-2271.
Johansen, G. & E. Sakshaug. 1993. Bio-optical characteristics and photoadaptative responses in the toxic and blooms-forming dinoflagellates Gyrodinium aureolum, Gymnodinium galatheaum, and two strains of Prorocentrum minimum. J. Phycol. 29: 267-642.
Kiefer, D. & R. Lasker. 1975. Two blooms of Gymnodinium splendens an unarmored dinoflagellate. Fish. Bull. 73: 675-678.
Lechuga-Deveze, C. & L. Morquecho-Escamilla. 1998. Early spring potentially harmful phytoplankton in Bahia Concepción, Gulf of California. Bull. Mar. Sci. 63: 503-512.
López-Cortés, D., J.J. Bustillos-Guzmán, I. Gárate-Lizárraga, F.E. Hernández-Sandoval & I. Murillo-Murillo. 2003. Phytoplankton biomasses and hydrographic conditions during El Niño 1997-1998 in Bahía Concepción, Gulf of California. Geophysica Inter. 42: 495-504.
Mackey, M.D., D.J. Mackey, H.W. Higgins & S.W. Wright. 1996. CHEMTAX- a program for estimating class abundances from chemical markers: application to HPLC measurements of phytoplankton. Mar. Ecol. Progr. Ser. 144: 265-283.
Mantoura, R.F.C. & C.A. Llewellyn. 1983. The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high-performance liquid chromatography. Anal. Chim. Acta 151:297-314.
Mantoura, R.F.C. & D. Repeta. 1997. Calibration methods for HPLC, pp. 407-428 In S.W. Jeffrey, R.F.C. Mantoura & S.W. Wright (eds.). Phytoplankton pigments in oceanography. Monographs in Oceanography Methodology 10. UNESCO.
Mee, L.D., M. Espinosa & G. Díaz. 1986. Paralytic shellfish poisoning with a Gymnodinium catenatum red tide on the pacific coast of Mexico. Mar. Environ. Res. 19:17-92.
Millan-Nuñez, E. 1988. Marea roja en Bahía de Los Ángeles. Cien. Mar. 14: 51-55. Park, J.G., M.K. Jeong, J.A. Lee, K.J. Cho & O.S. Kwon. 2001. Diurnal vertical migration of a harmful dinoflagellate, Cochlodinium polykrikoides (Dinophyceae), during a red tide in coastal waters of Namhae Island, Korea. Phycology 40: 292-297.
Smayda, T.J. 1997. Harmful algal blooms: Their ecophysiology and general relevance to phytoplankton blooms in the sea. Limnol. Oceanogr. 42:1137:1153.
Tang, E. 1996. Why dinoflagellates have lower growth rates? J. Phycol. 32: 80-84.
Vidussi, F., H. Claustre, J. Bustillos-Guzmán, C. Cailleau & J.C. Marty. 1996. Rapid HPLC method for determination of phytoplankton chemotaxinomic pigments: separation of chlorophyll a from divinyl-chlorophyll a and zeaxanthin from lutein. J. Plankton Res. 18: 2377-2382.
Yacobi, Y. Z., U. Pollingher, Y. Gonen, V. Gerhardt & A. Sukenik. 1996. HPLC analysis of phytoplankton pigments from Lake kinneret with special reference to the bloom-forming dinoflagellate Peridinium gatunense (Dinophyceae) and chlorophyll degradation products. J. Plankton Res. 18: 1781-1796.
Zapata, M., J. Freire & J.L.Garrido. 1998. Pigment composition of several harmful algae as determined by HPLC using pyridine-containing mobile phase polymeric octadecylsilica column, pp. 304-307. In B. Reguera, J. Blanco, M.L. Fernández & T. Wyatt (eds.). Harmful Algae. Xunta de Galicia and IOCUNESCO, Spain.
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