Currently, nitrogen has become the main element of water pollution, causing riverine, lacustrine and coastal eutrophication. The continuous contamination of aquifers and the absence of planned water resource utilization, boost its scarcity, and has been the only way in which our societies become aware of the urgent need to process the generated wastewater. The objective of this research was to evaluate the nitrifying capacity of different autochthonous bacterial isolates from soils from nearby sources of domestic wastewater drainage. For this, bacteria were isolated from Pirro River, contaminated with nitrogen of domestic sewage. Nitrifying bacteria were counted by serial dilution and agar plates, and were isolated until obtaining axenic colonies. These were identified by biochemical batteries or genetic sequencing, and the quantification of their nitrifying capacity was obtained by the methods 4500- NH4 + -F and 4500-NO-2-B, all between September 26, 2011 and March 16, 2014. A total of seven strains of nitrifying microorganisms were isolated and purified, including four Streptomyces sp., one Pseudomonas putida, one Sphingomonas sp. and one Aeromonas sp. We found that there were 2.23 x 105 UFC/g of soil of ammonium oxidizing bacteria and 2.2 x 104 CFU/g of soil of nitrite oxidizing bacteria in the samples. The quantification of the nitrifying capacity of the strains by colorimetric methods, determined that the maximum ammonium removal capacity was 0.050 mg N/L/day and 0.903 mg N/L/day of nitrite. The collection of few strains of nitrifying organisms and a low CFU count, can be attributed to the technique used, since this only recovers 1 % of the microorganisms present in a sample, which, however, is acceptable for studies which main purpose is to obtain cultivable microorganisms. Future research should consider removal tests with higher ammonium and nitrite levels, to find the maximum capacity of the isolated microorganisms, and evaluate their potential use in wastewater treatment systems.
Benavides, J. L., Quintero, G. M., & Ostos, O. L. (2006). Aislamiento e identificación de diez cepas bacterianas desnitrificantes a partir de un suelo agrícola contaminado con abonos nitrogenados proveniente de una finca productora de cebolla en la Laguna de Tota, Boyacá, Colombia. Nova-Publicación Científica, 4, 50-54.
Bitton, G. (2005). Wastewater microbiology (3rd ed). New Jersey, USA: Wiley-Liss.
Cervantes, F., Pérez, J., & Gómez, J. (2000). Avances en la eliminación biológica del nitrógeno de las aguas residuales. Revista Latinoamericana de Microbiología, 42, 73-82.
Coleman, D., Crossley, C. D. A., & Hendrix, P. F. (2004). Fundamentals of soil ecology (2nd Ed.). California, USA: Elsevier Academic Press.
Constantine, T. (2008). An overview of Ammonia and Nitrogen removal in wastewater treatment. Canada: CH2M HILL
Eaton, A., Clesceri, L., & Greenberg, A. (1995). Standard methods for the examination of water and wastewater (19th Ed.). Washington, DC: American Health Association.
Eldor, A. P. (2007). Soil microbiology, ecology and biochemistry (3rd Ed.). Miami, FL: Elsevier Academic Press.
Essington, M. E. (2004). Soil and Water Chemistry. An integrative Approach. Florida, USA: CRC Press.
Fiencke, C., Spieck, E., & Bock, E. (2005). Nitrifying Bacteria. In D. Werner & W. E. Newton (Eds.), Nitrogen fixation in Agriculture, Forestry, Ecology, and the Environment (255-276 pp.). Netherlands: Springer.
Garza, Y., Mata, J. C., Barbosa, L. C., & Rodríguez, J. (2001). Aislamiento y caracterización biodegradativa de microorganismos presentes en un lodo anaerobio. México: Universidad Autónoma de Coahuila. Recuperado de http://www.smbb.com.mx/congresos%20smbb/veracruz01/XIV_fisiolybioqmicroycel.html
Helmer, R., & Hespanhol, I. (1997). Water Pollution Control: A guide to the use of water quality management principles. London, UK: UNESCO/WHO/UNEP.
Hu, B. L., Zheng, P., Wu, X. Y., & Yin, L. (2005). Identification of ammonia oxidation Streptomyces strain A2 and study of its autotrophic ammonium oxidation characteristics. Act of Clinical Microbiology, 45(3), 321-4.
Jarpa, M., Aguilar, A., Belmonte, M., Decap, J., Abarzúa, M., & Vidal, G. (2007). Determinación de la capacidad nitrificante de un sedimento marino proveniente de un centro de cultivo de salmones. Interciencia, 32, 679-685.
Kanehisa Laboratories. (2013a). Kegg Pathway: Nitrogen metabolism: Aeromonas hydrophila subsp. hydrophila ATCC 7966. Retrieved from http://www.kegg.jp/kegg-bin/highlight_pathway?%20Scale%20=1.0&map=aha00910&keyword=Ammonia
Kanehisa Laboratories. (2013b). Kegg Pathway. Nitrogen metabolism: Sphingomonas wittichii. Retrieved from http://www.kegg.jp/kegg-bin/highlight_pathway?%20Scale=1.0&map=swi00910&keyword=Ammonia
Madigan, M., Martinko, T., Dunlap, J. M., & Clark, P. V. D. P. (2009). Brock Biología de los Microorganismos (12th ed). Madrid, Spain: Pearson Education.
Moat, A. G., Foster, J., & Spector, M. (2002). Microbial Physiology (4th Ed.). New York, USA: Wiley-Liss.
Mohan, S. B., Schmid, M., Jetten, M., & Cole, J. (2004). Detection and widespread distribution of the nrfA gene encoding nitrite reduction to ammonia, a short circuit in the biological nitrogen cycle that competes with denitrification. FEMS Microbiology Ecology, 49, 433-443.
Obaton, M., Amarger, N., & Alexander, M. (1968). Heterotrophic nitrification by Pseudomonas aeruginosa. Archives of Microbiology, 63, 122-132.
Okpokwasili, G., & Eleke, F. (1997). Effect of antimicrobial agents on the activity and survival of Aeromonas hydrophila and nitrifying bacteria in vitro. Journal of Natural Science Council of Sri Lanka, 25(4), 231-240.
Park, W., Nam, Y., Lee, M., & Kim, T. (2009). Anaerobic ammonia-oxidation coupled with Fe3+ reduction by an anaerobic culture from a piggery wastewater acclimated to NH4+/Fe3+ medium. Biotechnology and Bioprocess Engineering, 14, 680-685.
Spieck, E. & Bock, E. (2005). The Proteobacteria, Part Introductory Essays. In G. Garrity (Ed.), The lithoautotrophic nitrite-oxidizing bacteria. Bergey’s Manual of Systematic Bacteriology (pp. 149-153). New York, USA: Springer.
Templeton, M., & Butler, D. (2011). Introduction to Wastewater Treatment. UK: Imperial college London and University of Exeter, Ventus Publishing Aps.
Wehrfritz, J. M., Carter, J. P., Spiro, S., & Richardson, D. J. (1997). Hydroxylamine oxidation in heterotrophic nitrate-reducing soil bacteria and purification of a hydroxylamine-cytochrome c oxidoreductase from a Pseudomonas species. Archives of Microbiology, 166, 421-424.
Werner, D., & Newton, W. E. (2005). Nitrogen fixation in Agriculture, Forestry, Ecology and the Environment. Netherlands: Springer.
Witzel, K., & Overbeck, H. (1979). Heterotrophic nitrification by Arthrobacter sp. (strain 9006) as influenced by different cultural conditions, growth state and acetate metabolism. Archives of Microbiology, 122, 137-143.
Zhang, J., Wu, P., Hao, B., & Yu, Z. (2011). Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001. Bioresource Technology, 102, 9866-9869.