@article{Solís-Ramos_Ortiz-Pavón_Andrade-Torres_Porras-Murillo_Brenes-Angulo_Castaño de la Serna_2019, title={Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca}, volume={67}, url={https://revistas.ucr.ac.cr/index.php/rbt/article/view/37208}, DOI={10.15517/rbt.v67i2SUPL.37208}, abstractNote={<p class="p1">Common bean is a crop recalcitrant to <em>in vitro </em>regeneration and therefore it lacks an efficient transformation protocol that can be reproduced using <em>A. tumefaciens</em>. The main goal of this study was to establish a protocol for <em>A. tumefaciens</em> mediated transformation of <em>Phaseolus vulgaris</em> var. Brunca by marker genes (<em>gusA</em> and <em>nptII</em>) together with the gene for trehalose-6-phosphate synthase from <em>Saccharomyces cerevisiae</em> (<em>TPS1</em>) used in other species to increase tolerance to abiotic stress. The β-glucuronidase activity was detected in 45 % of the LBA4404 ElectroMAX® pCAMBIA1301 infected explants. Transformed explants regenerated new shoots after four to five months period in a kanamycin rich media. Surviving plants were evaluated by PCR and presented an 0.5 % efficiency of transformation. The established protocol for genetic transformation of common bean has two additional advantages with respect to previous reports: (1) it allows for obtaining transformed regenerants and (2) the genetic transformation was stable for the selective gene.</p>}, number={S2}, journal={Revista de Biología Tropical}, author={Solís-Ramos, Laura-Yesenia and Ortiz-Pavón, Julio-César and Andrade-Torres, Antonio and Porras-Murillo, Romano and Brenes-Angulo, Arturo and Castaño de la Serna, Enrique}, year={2019}, month={Apr.}, pages={S83–S94} }