TY - JOUR AU - Aguilar Diaz, Trinidad AU - Bertolini, Vincenzo AU - Carrillo Castañeda, Guillermo AU - Guillén Navarro, Griselda Karina AU - García Fajardo, Luz Verónica AU - Castro Chan, Ricardo Alberto PY - 2018/09/01 Y2 - 2024/03/28 TI - Growth-promoting rhizobacteria from Guarianthe skinneri (Orchidaceae). JF - Revista de Biología Tropical JA - Rev. Biol. Trop. VL - 66 IS - 3 SE - Articles DO - 10.15517/rbt.v66i3.30638 UR - https://revistas.ucr.ac.cr/index.php/rbt/article/view/30638 SP - 953–968 AB - <p>The <em>Guarianthe skinneri</em> orchid is included in NOM-059-ECOL-2010, Mexico standard as an endangered species. In order to study PGPR (promoting growth plant rhizobacteria) from this orchid, 10 roots were collected from different plants to isolate bacteria associated with the roots, which were analyzed by <em>in vitro</em> tests such as: production of AIA, nitrogen fixation, interaction with the mycorrhizal fungus <em>Thanatephorus </em>sp. strain RG26 and phosphate solubilization. We obtain 71 bacterial isolates, 10 strains of them were characterized by sequencing with the 16d rDNA marker identifying six bacteria: <em>Sphingomonas</em> sp. <em>Sinorhizobium</em> sp. <em>Bacillus</em> sp. <em>Nocardia cerradoensis</em>, <em>Bacillus megaterium</em> and <em>Burkholderia phytofirmans</em>. We observed that the bacterium <em>Sinorhizobium</em> sp. produced a greater amount of AIA (69.189 μg/ml) and <em>Bacillus</em> sp. performed greater acetylene reduction (10.251 nmol cultivo/96h). In the interactions of the bacteria and the fungus RG26, four categories were presented (extremely positive, positive, antagonism 50-50 and inhibition). In relation to the solubilization of phosphate, <em>Burkholderia phytofirmans</em> presented higher IS after 48 and 96 hr with an IS of 3.11 and 3.48, respectively. The results indicate that <em>Bacillus</em> sp. it could have the best characteristics to promote the development of the <em>G. skinneri</em> orchid by inoculating seeds and seedlings.</p><p><strong>&nbsp;</strong></p> ER -