TY - JOUR AU - Solís-Ramos, Laura-Yesenia AU - Ortiz-Pavón, Julio-César AU - Andrade-Torres, Antonio AU - Porras-Murillo, Romano AU - Brenes-Angulo, Arturo AU - Castaño de la Serna, Enrique PY - 2019/04/01 Y2 - 2024/03/29 TI - Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca JF - Revista de Biología Tropical JA - Rev. Biol. Trop. VL - 67 IS - S2 SE - Articles DO - 10.15517/rbt.v67i2SUPL.37208 UR - https://revistas.ucr.ac.cr/index.php/rbt/article/view/37208 SP - S83–S94 AB - <p class="p1">Common bean is a crop recalcitrant to <em>in vitro </em>regeneration and therefore it lacks an efficient transformation protocol that can be reproduced using <em>A. tumefaciens</em>. The main goal of this study was to establish a protocol for <em>A. tumefaciens</em> mediated transformation of <em>Phaseolus vulgaris</em> var. Brunca by marker genes (<em>gusA</em> and <em>nptII</em>) together with the gene for trehalose-6-phosphate synthase from <em>Saccharomyces cerevisiae</em> (<em>TPS1</em>) used in other species to increase tolerance to abiotic stress. The β-glucuronidase activity was detected in 45 % of the LBA4404 ElectroMAX® pCAMBIA1301 infected explants. Transformed explants regenerated new shoots after four to five months period in a kanamycin rich media. Surviving plants were evaluated by PCR and presented an 0.5 % efficiency of transformation. The established protocol for genetic transformation of common bean has two additional advantages with respect to previous reports: (1) it allows for obtaining transformed regenerants and (2) the genetic transformation was stable for the selective gene.</p> ER -