TY - JOUR AU - Pérez Méndez, Raquel AU - Jiménez Quesada, Karol AU - Garro Monge, Giovanni PY - 2022/01/24 Y2 - 2024/03/28 TI - In vitro culture of hairy roots of the shrub, Phyllanthus acuminatus (Phyllanthaceae). JF - Revista de Biología Tropical JA - Rev. Biol. Trop. VL - 70 IS - 1 SE - Articles DO - 10.15517/rev.biol.trop..v70i1.49227 UR - https://revistas.ucr.ac.cr/index.php/rbt/article/view/49227 SP - 647–657, e49227 AB - <p><strong>Introduction:</strong> Under natural conditions, the roots of the shrub, <em>Phyllanthus acuminatus</em>, produce low concentrations of secondary metabolites of medicinal interest. This opens an opportunity for in vitro culture, to increase metabolite concentration. <strong>Objective:</strong> To determine the optimal liquid culture conditions for hairy roots of <em>P. acuminatus. </em><strong>Methods:</strong> We used biomass growth evaluation according to initial inoculum percentage (0.50 and 0.10 %), percentage of medium nutrients (100, 50 and 25 %) and agitation rate (90, 100 and 110 min-1) (N=15 replications). <strong>Results:</strong> The best liquid culture conditions were: 0.10 % of initial inoculum, nutrients at 25 % and 90 min-1 for the agitation rate. There are differences among hairy roots and non-transformed roots. <strong>Conclusions:</strong> it is feasible to produce <em>P. acuminatus</em> hairy roots at a large scale, applying and implementing the evaluated conditions of inoculum percentage, nutrients in the medium and agitation rates.</p> ER -