Environmental DNA exploration of fish diversity in the Balantak River, Central Sulawesi, Indonesia
DOI:
https://doi.org/10.15517/hp29s516Keywords:
Amphidromy, Gobiiformes, metabarcoding, riverine biodiversity, WallaceaAbstract
Introduction: Freshwater ecosystems play a crucial role in supporting human life, providing many ecosystem services. Species diversity is a valuable indicator for evaluating ecosystem health, gaining a broader perspective on ecological richness and ecosystem functions, but data are lacking for many rivers. Objectives: To obtain baseline data on the upstream and downstream ichthyofauna of the Balantak River, Banggai Regency, using environmental DNA (eDNA) with a focus on diadromous species. Methods: We collected, filtered and preserved water samples from upstream and downstream sites on the Balantak River followed by eDNA analysis (DNA extraction, PCR with MiFish 12S rRNA primers, Illumina sequencing, MitoFish bioinformatic analysis at 97 and 99 % identity thresholds) to produce taxonomically assigned Actinopteri amplicon sequence variants (ASVs). Results: Our data are consonant with the expected dominance of diadromous and euryhaline taxa (75 %), high Gobiiformes biodiversity, and differences between upstream and downstream ichthyofaunal communities. Reads were assigned to Actinopteri ASVs from 12 orders, predominantly Gobiiformes, Anguilliformes, and Centrarchiformes. The 15 assigned families included Anguillidae, Eleotridae, Gobiidae, Rhyacichthydae, Kuhliidae, and Terapontidae. Of the assigned genera (17 at 97 % and 25 at 99 %), Anguilla had the most reads (39.9 %), and Sicyopterus had the most ASVs (8). Conclusions: These baseline data demonstrate the usefulness of eDNA metabarcoding for exploring ichthyofaunal biodiversity in small tropical watersheds while highlighting gaps in 12S rRNA sequence databases, reinforcing the need for integrative taxonomic research in Sulawesi watersheds and reliable reference databases. Most assigned taxa are vulnerable to exploitation for food and/or the ornamental fish trade. Four non-native taxa from Cichlidae, Cyprinidae and Clariidae are considered invasive. Implications for conservation include the need to address the unregulated and largely unreported freshwater ornamental trade and introduction of non-native invasive species.
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Albert, J. S., Destouni, G., Duke-Sylvester, S. M., Magurran, A. E., Oberdorff, T., Reis, R. E., Winemiller, K. O., & Ripple, W. J. (2021). Scientists’ warning to humanity on the freshwater biodiversity crisis. Ambio, 50(1), 85–94. https://doi.org/10.1007/s13280-020-01318-8
Ambo-Rappe, R., & Moore, A. M. (2019). Sulawesi Seas, Indonesia. In C. Sheppard (Ed.), World Seas: An Environmental Evaluation (2nd ed., pp. 559–581). Elsevier. https://doi.org/10.1016/B978-0-08-100853-9.00032-4
Aoyama, J., Wouthuyzen, S., Miller, M. J., Sugeha, H. Y., Kuroki, M., Watanabe, S., Syahailatua, A., Tantu, F. Y., Hagihara, S., Triyanto, T., Otake, T., & Tsukamoto, K. (2018). Reproductive ecology and biodiversity of freshwater eels around Sulawesi Island Indonesia. Zoological Studies, 57, 30. https://doi.org/10.6620/ZS.2018.57-30
Arai, T., Aoyama, J., Limbong, D., & Tsukamoto, K. (1999). Species composition and inshore migration of the tropical eels Anguilla spp. recruiting to the estuary of the Poigar River, Sulawesi Island. Marine Ecology Progress Series, 188, 299–303. https://doi.org/10.3354/meps188299
Arai, T. (2016). Biology and ecology of anguillid eels. CRC Press.
Astuti, S. S., Hariati, A. M., Kusuma, W. E., Yuniarti, A., Kurniawan, N., & Wiadnya, D. G. R. (2023). Anthropogenic introduction of the spotted barb, Barbodes binotatus, across the Wallace Line in western Sulawesi, Indonesia. Biodiversitas, 24(3), 1916–1925. https://doi.org/10.13057/biodiv/d240369
Bartholomew, D. C., Mosyaftiani, A., Morgan, B., Shah, T., Shaw, K., Stillman, C., Baldwin, K., Baqueiro, L. H. R., Birkinshaw, C., Breman, E., Diniz, G., Fereira, P., Flowers, C., Gichira, A., Jenkins, J., Kindt, R., Molin, P. G., Mungai, T., & Gann, G. D. (2024). The global biodiversity standard: Manual for assessment and best practices. Botanic Gardens Conservation International & Society for Ecological Restoration.
Bernery, C., Bellard, C., Courchamp, F., Brosse, S., Gozlan, R. E., Jarić, I., Teletchea, F., & Leroy, B. (2022). Freshwater fish invasions: A comprehensive review. Annual Review of Ecology, Evolution, and Systematics, 53(1), 427–456. https://doi.org/10.1146/annurev-ecolsys-032522-015551
Boseto, D. (2015). Sicyopterus marquesensis. The IUCN Red List of Threatened Species 2015: e.T196365A2449948. https://doi.org/10.2305/IUCN.UK.2012.RLTS.T196365A2449948.en
Bru, E. (2022). Planiliza subviridis, greenback mullet. The IUCN Red List of Threatened Species. https://doi.org/10.2305/IUCN.UK.2022-1.RLTS.T197050A2478575.en
Chen, X., Li, S., Zhao, J., & Yao, M. (2024). Passive eDNA sampling facilitates biodiversity monitoring and rare species detection. Environment International, 187, 108706. https://doi.org/10.1016/j.envint.2024.108706
Chua, K. W. J., & Lim, K. (2019). Barbodes binotatus, common barb. The IUCN Red List of Threatened Species. https://doi.org/10.2305/IUCN.UK.2019-2.RLTS.T169538A90996154.en
Drouineau, H., Durif, C., Castonguay, M., Mateo, M., Rochard, E., Verreault, G., Yokouchi, K., & Lambert, P. (2018). Freshwater eels: A symbol of the effects of global change. Fish and Fisheries, 19(5), 903–930. https://doi.org/10.1111/faf.12300
Dudgeon, D. (2019). Multiple threats imperil freshwater biodiversity in the Anthropocene. Current Biology, 29(19), R960–R967. https://doi.org/10.1016/j.cub.2019.08.002
Dudgeon, D. (2020). Freshwater biodiversity. Cambridge University Press. https://doi.org/10.1017/9781139032759
Durand, J. D., Hubert, N., Shen, K.-N., & Borsa, P. (2017). DNA barcoding grey mullets. Reviews in Fish Biology and Fisheries, 27(1), 233–243. https://doi.org/10.1007/s11160-016-9457-7
Freyhof, J., Sparks, J. S., Kaymaram, F., Feary, D., Bishop, J., Al-Husaini, M., Almukhtar, M., Hartmann, S., Alam, S., & Al-Khalaf, K. (2019). Chanos chanos, milkfish (amended version). The IUCN Red List of Threatened Species. https://doi.org/10.2305/IUCN.UK.2019-2.RLTS.T60324A151598011.en
Fricke, R., Eschmeyer, W. N., & Van der Laan, R. (Eds.). (2025). Eschmeyer’s catalog of fishes: Genera, species, references, electronic version. California Academy of Sciences. http://researcharchive.calacademy.org/research/ichthyology/catalog/fishcatmain.asp
Froese, R., & Pauly, D. (Eds.). (2025). FishBase: The global database of fishes. https://www.fishbase.se/
Gani, A., Bakri, A. A., Adriany, D. T., Nurjirana, N., Herjayanto, M., Bungalim, M. I., Ndobe, S., & Burhanuddin, A. I. (2019). Identification of freshwater goby species from the Biak and Koyoan Rivers, Luwuk Banggai, Central Sulawesi. Jurnal Ilmu Kelautan SPERMONDE, 5(2), 57–60. https://doi.org/10.20956/jiks.v5i2.8931
Gani, A., Nurjirana, N., Bakri, A. A., Adriany, D. T., Wuniarto, E., Khartiono, L. D., Satria, D. H., Hasan, V., Herjayanto, M., Burhanuddin, A. I., Moore, A. M., & Kobayashi, H. (2021). First record of Stiphodon annieae Keith & Hadiaty, 2015 (Teleostei, Oxudercidae) from Sulawesi Island, Indonesia. Check List, 17(1), 261–267. https://doi.org/10.15560/17.1.261
Gani, A., Adam, M. I., Bakri, A. A., Adriany, D. T., Herjayanto, M., Nurjirana, Mangitung, S. F., & Andriyono, S. (2021). Diversity studies of freshwater goby species from three river ecosystems in Luwuk Banggai, Central Sulawesi, Indonesia. IOP Conference Series: Earth and Environmental Science, 718(1), 012087. https://doi.org/10.1088/1755-1315/718/1/012087
Hagihara, S., Aoyama, J., Limbong, D., & Tsukamoto, K. (2018). Interspecific and sexual differences in riverine distribution of tropical eels Anguilla spp. Journal of Fish Biology, 93(1), 21–29. https://doi.org/10.1111/jfb.13666
Hansen, H. H., Bergman, E., Cowx, I. G., Lind, L., Pauna, V. H., & Willis, K. A. (2023). Resilient rivers and connected marine systems: A review of mutual sustainability opportunities. Global Sustainability, 6, e2. https://doi.org/10.1017/sus.2022.19
Haryono, H., & Sauri, S. (2020). Komunitas ikan di perairan tawar wilayah Kabupaten Banggai Kepulauan Sulawesi Tengah. Sriwijaya Bioscientia, 1(1), 1–7. https://doi.org/10.24233/sribios.1.1.2020.164
Haÿ, V., Mennesson, M. I., Lord, C., & Keith, P. (2023). Why several when one can unite them all? Integrative taxonomic revision of Indo-Pacific freshwater pipefish (Nerophinae). Zoological Journal of the Linnean Society, 198(4), 923–956. https://doi.org/10.1093/zoolinnean/zlad007
Herder, F., Schliewen, U., Geiger, M., Hadiaty, R., Gray, S., McKinnon, J., Walter, R., & Pfaender, J. (2012). Alien invasion in Wallace’s Dreamponds: Records of the hybridogenic “flowerhorn” cichlid in Lake Matano, with an annotated checklist of fish species introduced to the Malili Lakes system in Sulawesi. Aquatic Invasions, 7(4), 521–535. https://doi.org/10.3391/ai.2012.7.4.009
Herder, F., Möhring, J., Flury, J. M., Utama, V., Wantania, L., Wowor, D., Boneka, F. B., Stelbrink, B., Hilgers, L., Schwarzer, J., & Pfaender, J. (2022). More non-native fish species than natives, and an invasion of Malawi cichlids, in ancient Lake Poso, Sulawesi, Indonesia. Aquatic Invasions, 17(1), 72–91. https://doi.org/10.3391/ai.2022.17.1.05
Herjayanto, M., Gani, A., Adel, Y. S., & Suhendra, N. (2019). Freshwater fish of lakes and its inlet rivers in Sulawesi Tengah Province, Indonesia. Journal of Aquatropica Asia, 4(1), 1–9. https://doi.org/10.33019/aquatropica.v4i1.1679
Hidayani, A. A., Tassaka, A. C. M. A. R., Kadir, N. N., Yasir, I., Jompa, J., Umar, W., Litaay, M., Parenrengi, A., Ndobe, S., Gani, A., Putri, A. P., Iqram, M., Omar, S. B. A., & Moore, A. M. (2024). First DNA barcodes of two endemic fishes from the Maros karst region, South Sulawesi, Indonesia. Cybium, 48(3), 247–250. https://doi.org/10.26028/CYBIUM/2024-015
Hilgers, L., Herder, F., Hadiaty, R. K., & Pfaender, J. (2018). Alien attack: Trophic interactions of flowerhorn cichlids with endemics of ancient Lake Matano (Sulawesi, Indonesia). Evolutionary Ecology Research, 19(5), 575–590. http://www.evolutionary-ecology.com/issues/v19/n05/jjar3112.pdf
Hoese, D., Mailautoka, K., & Ebner, B. (2019). Kuhlia marginata, silver flagtail. The IUCN Red List of Threatened Species. https://doi.org/10.2305/IUCN.UK.2019-3.RLTS.T11060A123377956.en
Hubert, N., Kadarusman, Wibowo, A., Busson, F., Caruso, D., Sulandari, S., Nafiqoh, N., Pouyaud, L., Rüber, L., Avarre, J.-C., Herder, F., Hanner, R., Keith, P., & Hadiaty, R. K. (2015). DNA barcoding Indonesian freshwater fishes: Challenges and prospects. DNA Barcodes, 3(1), 144–169. https://doi.org/10.1515/dna-2015-0018
IPBES. (2023). Summary for policymakers of the thematic assessment report on invasive alien species and their control of the Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services (H. E. Roy et al., Eds.). IPBES Secretariat. https://doi.org/10.5281/zenodo.7430692
Iqram, M., Litaay, M., Hasyim, Z., Moka, W. J. C., & Tinungki, G. M. (2024). Combining eDNA metabarcoding and conventional method in rapid screening of fish diversity in Maros-Pangkep Global Geopark, South Sulawesi, Indonesia. Biodiversitas, 25(2), 819–828. https://doi.org/10.13057/biodiv/d250242
Ishihara, T., & Tachihara, K. (2008). Reproduction and early development of a freshwater pipefish Microphis leiaspis in Okinawa-jima Island, Japan. Ichthyological Research, 55(4), 349–355. https://doi.org/10.1007/s10228-008-0049-y
International Union for Conservation of Nature. (2025). The IUCN Red List of Threatened Species. https://www.iucnredlist.org
Iwasaki, W., Fukunaga, T., Isagozawa, R., Yamada, K., Maeda, Y., Satoh, T. P., Sado, T., Mabuchi, K., Takeshima, H., Miya, M., & Nishida, M. (2013). Mitofish and mitoannotator: A mitochondrial genome database of fish with an accurate and automatic annotation pipeline. Molecular Biology and Evolution, 30(11), 2531–2540. https://doi.org/10.1093/molbev/mst141
Jacoby, D. M. P., Casselman, J. M., Crook, V., Delucia, M., Ahn, H., Kaifu, K., Kurwie, T., Sasal, P., Silfvergrip, A. M. C., Smith, K. G., Uchida, K., Walker, A. M., & Gollock, M. J. (2015). Synergistic patterns of threat and the challenges facing global anguillid eel conservation. Global Ecology and Conservation, 4, 321–333. https://doi.org/10.1016/j.gecco.2015.07.009
Jenkins, A., Kullander, F. F., Tan, H. H., Sparks, J. S., & Pollom, R. (2017). Microphis leiaspis, barhead pipefish. The IUCN Red List of Threatened Species. https://doi.org/10.2305/IUCN.UK.2017-2.RLTS.T169358A58318427.en
Keith, P., Allen, G., Lord, C., & Hadiaty, R. (2011). Five new species of Sicyopterus (Gobioidei: Sicydiinae) from Papua New Guinea and Papua. Cybium, 35(4), 299–318. https://doi.org/10.26028/CYBIUM/2011-354-004
Keith, P., Hadiaty, R., Hubert, N., Busson, F., & Lord, C. (2014). Three new species of Lentipes from Indonesia (Gobiidae). Cybium, 38(2), 133–146. https://doi.org/10.26028/cybium/2014-382-004
Keith, P., & Lord, C. (2012). Tropical freshwater gobies: Amphidromy as a life cycle. In R. A. Patzner, J. L. Van Tassell, M. Kovačić, & B. G. Kapoor (Eds.), The biology of gobies (pp. 243–277). CRC Press. https://doi.org/10.1201/b11397-18
Keith, P., Lord, C., Busson, F., Sauri, S., Hubert, N., & Hadiaty, R. (2015). A new species of Sicyopterus (Gobiidae) from Indonesia. Cybium, 39(4), 243–248. https://doi.org/10.26028/cybium/2015-393-005
Keith, P., Lord, C., & Maeda, K. (2014). Indo-Pacific sicydiine gobies: Biodiversity, life traits and conservation. Société Française d’Ichtyologie.
Keith, P., Mennesson, M. I., Sauri, S., Busson, F., Delrieu-Trottin, E., Limmon, G., Nurjirana, Dahruddin, H., & Hubert, N. (2020). Giuris (Teleostei: Eleotridae) from Indonesia, with description of a new species. Cybium, 44(4), 317–329. https://doi.org/10.26028/cybium/2020-444-003
Kennard, M., Brooks, S., Kerezsy, A., & Ebner, B. (2019). Leiopotherapon unicolor. The IUCN Red List of Threatened Species. e.T122906520A123382321. https://doi.org/10.2305/IUCN.UK.2019-3.RLTS.T122906520A123382321.en
Kottelat, M., Whitten, A. J., Kartikasari, S., & Wirjoatmodjo, S. (1993). Freshwater fishes of western Indonesia and Sulawesi. Periplus.
Kottelat, M., & Whitten, A. J. (1996). Freshwater fishes of western Indonesia and Sulawesi: Additions and corrections. Periplus Editions.
Lagarde, R., Courret, D., Grondin, H., Faivre, L., & Ponton, D. (2021). Climbing for dummies: Recommendations for multi-specific fishways for the conservation of tropical eels and gobies. Animal Conservation, 24(6), 970–981. https://doi.org/10.1111/acv.12699
Lohman, D. J., de Bruyn, M., Page, T., von Rintelen, K., Hall, R., Ng, P. K. L., Shih, H.-T., Carvalho, G. R., & von Rintelen, T. (2011). Biogeography of the Indo-Australian Archipelago. Annual Review of Ecology, Evolution, and Systematics, 42, 205–226. https://doi.org/10.1146/annurev-ecolsys-102710-145001
Lord, C., Brun, C., Hautecœur, M., & Keith, P. (2010). Insights on endemism: Comparison of the duration of the marine larval phase estimated by otolith microstructural analysis of three amphidromous Sicyopterus species (Gobioidei: Sicydiinae) from Vanuatu and New Caledonia. Ecology of Freshwater Fish, 19(1), 26–38. https://doi.org/10.1111/j.1600-0633.2009.00386.x
Lumbantobing, D. (2019a). Oryzias celebensis. The IUCN Red List of Threatened Species. https://doi.org/10.2305/IUCN.UK.2019-2.RLTS.T15579A90980558.en
Lumbantobing, D. (2019b). Telmatherina celebensis. The IUCN Red List of Threatened Species. https://doi.org/10.2305/IUCN.UK.2019-2.RLTS.T21573A90980085.en
Lumbantobing, D., & Larson, H. (2019). Glossogobius flavipinnis. The IUCN Red List of Threatened Species. e.T40707A90982306. https://doi.org/10.2305/IUCN.UK.2019-2.RLTS.T40707A90982306.en
Lynch, A. J., Elliott, V., Phang, S. C., Claussen, J. E., Harrison, I., Murchie, K. J., Steel, E. A., & Stokes, G. L. (2020). Inland fish and fisheries integral to achieving the Sustainable Development Goals. Nature Sustainability, 3(8), 579–587. https://doi.org/10.1038/s41893-020-0517-6
Maasri, A., Jähnig, S. C., Adamescu, M. C., Adrian, R., Baigun, C., Baird, D. J., Batista-Morales, A., Bonada, N., Brown, L. E., Cai, Q., Campos-Silva, J. V., Clausnitzer, V., Contreras-MacBeath, T., Cooke, S. J., Datry, T., Delacámara, G., De Meester, L., Dijkstra, K. B., Do, V. T., … Worischka, S. (2022). A global agenda for advancing freshwater biodiversity research. Ecology Letters, 25(2), 255–263. https://doi.org/10.1111/ele.13931
Madduppa, H., & Sani, L. M. I. (2020). Environmental DNA sampling protocol. IPB University & Oceanogen.
Maeda, K., Kobayashi, H., Palla, H. P., Shinzato, C., Koyanagi, R., Montenegro, J., Nagano, A. J., Saeki, T., Kunishima, T., Mukai, T., Tachihara, K., Laudet, V., Satoh, N., & Yamahira, K. (2021). Do colour morphs of an amphidromous goby represent different species? Taxonomy of Lentipes (Gobiiformes) from Japan and Palawan, Philippines, with phylogenomic approaches. Systematics and Biodiversity, 19(8), 1080–1112. https://doi.org/10.1080/14772000.2021.1971792
Maruyama, A., Nakamura, K., Yamanaka, H., Kondoh, M., & Minamoto, T. (2014). The release rate of environmental DNA from juvenile and adult fish. PLoS ONE, 9(12), e114639. https://doi.org/10.1371/journal.pone.0114639
McElroy, M. E., Dressler, T. L., Titcomb, G. C., Wilson, E. A., Deiner, K., Dudley, T. L., Eliason, E. J., Evans, N. T., Gaines, S. D., Lafferty, K. D., Lamberti, G. A., Li, Y., Lodge, D. M., Love, M. S., Mahon, A. R., Pfrender, M. E., Renshaw, M. A., Selkoe, K. A., & Jerde, C. L. (2020). Calibrating environmental DNA metabarcoding to conventional surveys for measuring fish species richness. Frontiers in Ecology and Evolution, 8, 1–12. https://doi.org/10.3389/fevo.2020.00276
Mennesson, M. I., Bonillo, C., Feunteun, E., & Keith, P. (2018). Phylogeography of Eleotris fusca (Teleostei: Gobioidei: Eleotridae) in the Indo-Pacific area reveals a cryptic species in the Indian Ocean. Conservation Genetics, 19(5), 1025–1038. https://doi.org/10.1007/s10592-018-1063-x
Miesen, F. W., Droppelmann, F., Hüllen, S., Hadiaty, R. K., & Herder, F. (2016). An annotated checklist of the inland fishes of Sulawesi. Bonn Zoological Bulletin, 64(2), 77–106.
Miya, M., Sato, Y., Fukunaga, T., Sado, T., Poulsen, J. Y., Sato, K., Minamoto, T., Yamamoto, S., Yamanaka, H., Araki, H., Kondoh, M., & Iwasaki, W. (2015). MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: Detection of more than 230 subtropical marine species. Royal Society Open Science, 2(7), 150088. https://doi.org/10.1098/rsos.150088
Miya, M., Gotoh, R. O., & Sado, T. (2020). MiFish metabarcoding: A high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples. Fisheries Science, 86(6), 939–950. https://doi.org/10.1007/s12562-020-01461-x
Moore, A. M., Jompa, J., Tassaka, A. C. M. A. R., Yasir, I., Ndobe, S., Umar, W., Gold, Z., & Barber, P. H. (2021). Sharks and rays (Chondrichthyes) around Banggai Island, Banggai MPA, Indonesia: Biodiversity data from an environmental DNA pilot study. AACL Bioflux, 14(2), 725–745.
Moore, A. M., Yasir, I., Tassaka, A. C. M., Ndobe, S., Umar, W., Kadir, N. N., Putri, A. P., Yusuf, S., Barber, P. H., & Jompa, J. (2025). Perspectives from an eDNA metabarcoding pilot project in the Banggai MPA, Central Sulawesi, Indonesia. Bulletin of Marine Science, 101(1), 349–365. https://doi.org/10.5343/bms.2023.0125
Morley, J., Buchanan, G., Mitchard, E. T. A., & Keane, A. (2021). Implications of the World Bank’s environmental and social framework for biodiversity. Conservation Letters, 14(1), 1–6. https://doi.org/10.1111/conl.12759
Ndobe, S., Gani, A., Bakri, A. A., Adryani, D. T., Wuniarto, E., Khartiono, L. D., Herjayanto, M., Hasan, V., & Moore, A. M. (2022). First and recurrent records of Stiphodon surrufus Watson & Kottelat, 1995 (Gobiiformes, Gobiidae, Sicydiinae), a naturally rare amphidromous goby, in Sulawesi, Indonesia. Check List, 18(2), 253–260. https://doi.org/10.15560/18.2.253
Ndobe, S., Nurdin, M. S., Hasanah, N., Putra, A. E., Mansyur, K., Nasir, M., Rabuna, M. L., & Moore, A. M. (2023). DNA barcoding detects resurrected taxon Giuris laglaizei (Sauvage, 1880) in Sulawesi, Indonesia: Bolano Sau Lake payangka phylogeny, phenotypic characters and implications for Giuris spp. conservation. F1000Research, 11, 295. https://doi.org/10.12688/f1000research.108970.2
Ndobe, S., Gani, A., Herjayanto, M., Bandjolu, K. P., Mokodongan, D. F., Khartiono, L. D., & Moore, A. M. (2024). DNA barcode, sex ratio, and phenotypic characters of the Poso Lake (Sulawesi) endemic Mugilogobius sarasinorum (Boulenger, 1897). Cybium, 48(3), 291–294. https://doi.org/10.26028/cybium/2024-022
Nedopil, C., Larsen, M., Chane-Yook, A., & Narain, D. (2024). Catching up with climate priorities: Understanding multilateral development banks’ evolving approach to biodiversity. Global Policy, 15(4), 660–675. https://doi.org/10.1111/1758-5899.13403
Ng, H. H., & Low, B. W. (2019). Clarias batrachus. The IUCN Red List of Threatened Species. https://doi.org/10.2305/IUCN.UK.2019-2.RLTS.T166613A1138872.en
Nurjirana, Burhanuddin, A. I., & Haris, A. (2019). Diversity of penja fish (amphidromous goby) in Leppangan River, West Sulawesi, Indonesia. AACL Bioflux, 12(1), 246–249. http://www.bioflux.com.ro/docs/2019.246-249.pdf
Nurjirana, Keith, P., Burhanuddin, A. I., Haris, A., & Afrisal, M. (2021). DNA barcoding of two amphidromous goby postlarvae (‘penja’) morphotypes from Mandar River, West Sulawesi, Indonesia. Cybium, 45(3), 243–249. https://doi.org/10.26028/cybium/2021-453-009
Nurjirana, N., Burhanuddin, A. I., Keith, P., Haris, A., & Afrisal, M. (2022). Amphidromous goby postlarvae (penja) migration seasons and fisheries in West Sulawesi, Indonesia. Biodiversitas Journal of Biological Diversity, 23(1), 375–380. https://doi.org/10.13057/biodiv/d230138
Nurjirana, Burhanuddin, A. I., Haris, A., Moore, A. M., Gani, A., Hasan, V., Motomura, H., Kobayashi, H., Haryono, H., & Mokodongan, D. F. (2024). Occurrence and new data for the endemic amphidromous goby Lentipes mekonggaensis in Central Sulawesi, Indonesia. Cybium, 48(4), 303–307. https://doi.org/10.26028/cybium/2024-025
Oikonomou, A. (2021). Freshwater biogeography in a nutshell. In E. Guilbert (Ed.), Biogeography: An integrative approach of the evolution of living (pp. 219–243). ISTE Ltd & John Wiley & Sons, Inc. https://doi.org/10.1002/9781119882381.ch9
Ojaveer, H., Galil, B. S., Carlton, J. T., Alleway, H., Goulletquer, P., Lehtiniemi, M., Marchini, A., Miller, W., Occhipinti-Ambrogi, A., Peharda, M., Ruiz, G. M., Williams, S. L., & Zaiko, A. (2018). Historical baselines in marine bioinvasions: Implications for policy and management. PLOS ONE, 13(8), e0202383. https://doi.org/10.1371/journal.pone.0202383
Oliver, T. H., Heard, M. S., Isaac, N. J. B., Roy, D. B., Procter, D., Eigenbrod, F., Freckleton, R., Hector, A., Orme, C. D. L., Petchey, O. L., Proença, V., Raffaelli, D., Suttle, K. B., Mace, G. M., Martín-López, B., Woodcock, B. A., & Bullock, J. M. (2015). Biodiversity and resilience of ecosystem functions. Trends in Ecology & Evolution, 30(11), 673–684. https://doi.org/10.1016/j.tree.2015.08.009
Ottoni, F. P., South, J., Azevedo-Santos, V. M., Henschel, E., & de Bragança, P. H. N. (2023). Editorial: Freshwater biodiversity crisis: Multidisciplinary approaches as tools for conservation. Frontiers in Environmental Science, 11, 1155608. https://doi.org/10.3389/fenvs.2023.1155608
Pangemanan, N. P. L., Kepel, R. C., Bataragoa, N. E., Tumbol, R. A., Sahami, F. M., Lumenta, C., Salaki, M. S., Boneka, F. B., & Mantiri, D. M. H. (2020). Morphometric and molecular analysis for identification of the early stages of gobioid fishes in Poigar River estuary, North Sulawesi, Indonesia. AACL Bioflux, 13(5), 2639–2646.
Paramata, A. R., Tresnati, J., Kadir, N. N., & Moore, A. M. (2025). Native and introduced fish caught with gillnets in Limboto Lake, Gorontalo Province, Indonesia. Fisheries and Aquatic Sciences, 28(4), 236–247. https://doi.org/10.47853/FAS.2025.e21
Perry, W. B., Seymour, M., Orsini, L., Jâms, I. B., Milner, N., Edwards, F., Harvey, R., de Bruyn, M., Bista, I., Walsh, K., Emmett, B., Blackman, R., Altermatt, F., Lawson Handley, L., Mächler, E., Deiner, K., Bik, H. M., Carvalho, G., Colbourne, J., … Creer, S. (2024). An integrated spatio-temporal view of riverine biodiversity using environmental DNA metabarcoding. Nature Communications, 15(1), 4372. https://doi.org/10.1038/s41467-024-48640-3
Pike, C., Crook, V., Jacoby, D., & Gollock, M. (2020). Anguilla interioris (Highlands long-finned eel). The IUCN Red List of Threatened Species. e.T198972A176496665. International Union for Conservation of Nature. https://doi.org/10.2305/IUCN.UK.2020-3.RLTS.T198972A176496665.en
Porter, T. M., & Hajibabaei, M. (2018). Scaling up: A guide to high-throughput genomic approaches for biodiversity analysis. Molecular Ecology, 27(2), 313–338. https://doi.org/10.1111/mec.14478
Pyšek, P., Hulme, P. E., Simberloff, D., Bacher, S., Blackburn, T. M., Carlton, J. T., Dawson, W., Essl, F., Foxcroft, L. C., Genovesi, P., Jeschke, J. M., Kühn, I., Liebhold, A. M., Mandrak, N. E., Meyerson, L. A., Pauchard, A., Pergl, J., Roy, H. E., Seebens, H., … Richardson, D. M. (2020). Scientists’ warning on invasive alien species. Biological Reviews, 95(6), 1511–1534. https://doi.org/10.1111/brv.12627
Reid, A. J., Carlson, A. K., Creed, I. F., Eliason, E. J., Gell, P. A., Johnson, P. T. J., Kidd, K. A., MacCormack, T. J., Olden, J. D., Ormerod, S. J., Smol, J. P., Taylor, W. W., Tockner, K., Vermaire, J. C., Dudgeon, D., & Cooke, S. J. (2019). Emerging threats and persistent conservation challenges for freshwater biodiversity. Biological Reviews, 94(3), 849–873. https://doi.org/10.1111/brv.12480
Righton, D., Piper, A., Aarestrup, K., Amilhat, E., Belpaire, C., Casselman, J., Castonguay, M., Díaz, E., Dörner, H., Faliex, E., Feunteun, E., Fukuda, N., Hanel, R., Hanzen, C., Jellyman, D., Kaifu, K., McCarthy, K., Miller, M. J., Pratt, T., … Gollock, M. (2021). Important questions to progress science and sustainable management of anguillid eels. Fish and Fisheries, 22(4), 762–788. https://doi.org/10.1111/faf.12549
Riley, I. J., DiBattista, J. D., Stewart, J., Schilling, H. T., & Suthers, I. M. (2023). Using integrative taxonomy to distinguish cryptic halfbeak species and interpret distribution patterns, fisheries landings, and speciation. Marine and Freshwater Research, 74(2), 125–143. https://doi.org/10.1071/MF22048
Robin, F., Valen, S., Nomleni, A., Turnip, G., Luhulima, M. Y., & Insani, L. (2023). Presence of non-native freshwater fish in Indonesia: A review—risk and ecological impacts. AACL Bioflux, 16(1), 66–79. http://www.bioflux.com.ro/docs/2023.66-79.pdf
Robinson, C. V., Porter, T. M., Maitland, V. C., Wright, M. T. G., & Hajibabaei, M. (2022). Multi-marker metabarcoding resolves subtle variations in freshwater condition: Bioindicators, ecological traits, and trophic interactions. Ecological Indicators, 145, 109603. https://doi.org/10.1016/j.ecolind.2022.109603
Ruppert, K. M., Kline, R. J., & Rahman, M. S. (2019). Past, present, and future perspectives of environmental DNA (eDNA) metabarcoding: A systematic review in methods, monitoring, and applications of global eDNA. Global Ecology and Conservation, 17, e00547. https://doi.org/10.1016/j.gecco.2019.e00547
Sahami, F. M., Kepel, R. C., Olii, A. H., Pratasik, S. B., Lasabuda, R., Wantasen, A., & Habibie, S. A. (2020). Morphometric and genetic variations of species composition of nike fish assemblages in Gorontalo Bay waters, Indonesia. Biodiversitas Journal of Biological Diversity, 21(10), 4571–4581. https://doi.org/10.13057/biodiv/d211015
Sato, Y., Miya, M., Fukunaga, T., Sado, T., & Iwasaki, W. (2018). MitoFish and MiFish pipeline: A mitochondrial genome database of fish with an analysis pipeline for environmental DNA metabarcoding. Molecular Biology and Evolution, 35(6), 1553–1555. https://doi.org/10.1093/molbev/msy074
Serdiati, N., Arfiati, D., Widodo, M. S., Lelono, T. D., Ndobe, S., Mansyur, K., & Moore, A. M. (2020). Perspectives on sustainable management of the Poso Lake (Indonesia) endemic ricefish, Oryzias nigrimas (Actinopterygii: Adrianichthyidae). Revista de Biología Tropical, 69(1), 139–152. https://doi.org/10.15517/rbt.v69i1.42404
Serdiati, N., Ndobe, S., Rosyida, E., & Moore, A. M. (2023). Barcoding a glass eel migration: A snapshot of taxa migrating upstream in the Palu River, Central Sulawesi, Indonesia. IOP Conference Series: Earth and Environmental Science, 1272(1), 012020. https://doi.org/10.1088/1755-1315/1272/1/012020
Serdiati, N., Ndobe, S., Rosyida, E., Gani, A., Hermawan, R., Nurdin, M. S., Sari, D. E., Herlina, S., Pawaro, M. F. D., & Danty, A. R. (2024). Length-weight relationship, condition factor and DNA barcoding of bonylip barb, Osteochilus vittatus (Valenciennes, 1842) in Dampelas Lake, Central Sulawesi, Indonesia. Omni-Akuatika, 20(1), 23–37. https://doi.org/10.20884/1.oa.2024.20.1.1111
Shao, X., Fang, Y., Jawitz, J. W., Yan, J., & Cui, B. (2019). River network connectivity and fish diversity. Science of the Total Environment, 689, 21–30. https://doi.org/10.1016/j.scitotenv.2019.06.340
Shu, L., Ludwig, A., & Peng, Z. (2021). Environmental DNA metabarcoding primers for freshwater fish detection and quantification: In silico and in tanks. Ecology and Evolution, 11(12), 8281–8294. https://doi.org/10.1002/ece3.7658
Sorensen, P. W. (Ed.). (2021). Biology and control of invasive fishes. MDPI.
Stelbrink, B., Stöger, I., Hadiaty, R. K., Schliewen, U. K., & Herder, F. (2014). Age estimates for an adaptive lake fish radiation, its mitochondrial introgression, and an unexpected sister group: Sailfin silversides of the Malili Lakes system in Sulawesi. BMC Evolutionary Biology, 14(1), 94. https://doi.org/10.1186/1471-2148-14-94
Sugeha, H. Y. (2010). Recruitment mechanism of the tropical glass eel genus Anguilla in the Poso Estuary, Central Sulawesi Island, Indonesia. Jurnal Perikanan, 12(2), 86–100.
Takahashi, M., Saccò, M., Kestel, J. H., Nester, G., Campbell, M. A., van der Heyde, M., Heydenrych, M. J., Juszkiewicz, D. J., Nevill, P., Dawkins, K. L., Bessey, C., Fernandes, K., Miller, H., Power, M., Mousavi-Derazmahalleh, M., Newton, J. P., White, N. E., Richards, Z. T., & Allentoft, M. E. (2023). Aquatic environmental DNA: A review of the macro-organismal biomonitoring revolution. Science of the Total Environment, 873, 162322. https://doi.org/10.1016/j.scitotenv.2023.162322
Tauli, M., Garcia, M. P., Podeque, J. R., Signey, L., Sarmiento, K., & Santos, M. (2022). First record of the goby Lentipes armatus Sakai and Nakamura, 1979 (Teleostei: Gobiidae) in the Amburayan River in Benguet, Luzon Island, Philippines. The Philippine Journal of Fisheries, 29(1), 78–81. https://doi.org/10.31398/tpjf/29.1.2020C0015
Tedesco, P. A., Beauchard, O., Bigorne, R., Blanchet, S., Buisson, L., Conti, L., Cornu, J.-F., Dias, M. S., Grenouillet, G., Hugueny, B., Jézéquel, C., Leprieur, F., Brosse, S., & Oberdorff, T. (2017). A global database on freshwater fish species occurrence in drainage basins. Scientific Data, 4(1), 170141. https://doi.org/10.1038/sdata.2017.141
Tembo, J. K., Kihia, C. M., Kitaka, N. K., Brien, G. O., Hanzen, C., Mbaru, E., & Wanjiru, K. C. (2023). Diet and trophic interactions between catadromous eels and sympatric fish in Kenyan east flowing river systems. Western Indian Ocean Journal of Marine Science, 22(2), 87–106. https://doi.org/10.4314/wiojms.v22i2.7
Thinh, D. V., Van, N. S., Nguyen, T. H. T., & Lumbantobing, D. (2020). Barbonymus gonionotus, silver barb. The IUCN Red List of Threatened Species. https://doi.org/10.2305/IUCN.UK.2020-3.RLTS.T166914A89799952.en
Torres, A. G., Leander, N. J. S., Kesner-Reyes, K., Ballad, E. L., Ame, E. C., Palla, H. P., Angeles, I. J. P., Gonzalez, J. C. T., Reyes, A. T., Gaerlan, R. S. P., Guino-o, R. S. I., & Cecilio, M. A. F. (2021). Leiopotherapon plumbeus. The IUCN Red List of Threatened Species. https://doi.org/10.2305/IUCN.UK.2021-1.RLTS.T162161619A162161646.en
Triyanto, T., Haryani, G. S., Lukman, Wibowo, H., Ali, F., Hidayat, Sulawesty, F., Setiawan, F. A., Triwisesa, E., Dwinovantyo, A., Riyanto, M., Samir, O., & Nafisyah, E. (2021). Perspective plan for sustainable eel management in Lake Poso, Central Sulawesi. E3S Web of Conferences, 322, 05014. https://doi.org/10.1051/e3sconf/202132205014
Tweedley, J. R., Bird, D. J., Potter, I. C., Gill, H. S., Miller, P. J., O’Donovan, G., & Tjakrawidjaja, A. H. (2013). Species compositions and ecology of the riverine ichthyofaunas in two Sulawesian islands in the biodiversity hotspot of Wallacea. Journal of Fish Biology, 82(6), 1916–1950. https://doi.org/10.1111/jfb.12121
Vari, R. P., & Hadiaty, R. K. (2012). The endemic Sulawesi fish genus Lagusia (Teleostei: Terapontidae). Raffles Bulletin of Zoology, 60(1), 157–162.
Wang, B., Jiao, L., Ni, L., Wang, M., & You, P. (2024). Bridging the gap: The integration of eDNA techniques and traditional sampling in fish diversity analysis. Frontiers in Marine Science, 11, 1289589. https://doi.org/10.3389/fmars.2024.1289589
Wantania, L. L., Koppetsch, T., Möhring, J., Miesen, F. W., Wowor, D., Boneka, F., & Herder, F. (2024). Sulawesi stream fish communities depend on connectivity and habitat diversity. Journal of Fish Biology, 105(4), 1–18. https://doi.org/10.1111/jfb.15944
Williams-Subiza, E. A., & Epele, L. B. (2021). Drivers of biodiversity loss in freshwater environments: A bibliometric analysis of the recent literature. Aquatic Conservation: Marine and Freshwater Ecosystems, 31(9), 2469–2480. https://doi.org/10.1002/aqc.3627
WoRMS Editorial Board. (2025). World Register of Marine Species [Database]. https://doi.org/10.14284/170
Xu, Y., Huo, X., Chen, X., Wang, Z., Zhou, M., Zhu, J., Yan, R., & Cai, Y. (2024). Comparing the performance variability of different eDNA primers in fish monitoring. Watershed Ecology and the Environment, 6(5), 165–175. https://doi.org/10.1016/j.wsee.2024.08.003
Yanuarita, D., Inaku, D. F., Nurdin, N., Rahim, S. W., Kudsiah, H., Parawansa, B. S., Rukminasari, N., Irmawati, & Moka, W. (2020). Aquatic invasive species distribution within Wallace region: A preliminary review. IOP Conference Series: Earth and Environmental Science, 564(1), 012038. https://doi.org/10.1088/1755-1315/564/1/012038
Zhu, T., Sato, Y., Sado, T., Miya, M., & Iwasaki, W. (2023). MitoFish, MitoAnnotator, and MiFish pipeline: Updates in 10 years. Molecular Biology and Evolution, 40(3), 1–5. https://doi.org/10.1093/molbev/msad035
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