Using historical fish collections to track microplastic contamination in Costa Rican freshwater ecosystems
DOI:
https://doi.org/10.15517/rrpwc645Keywords:
Astyanax aeneus, freshwater fish, microplastic ingestion, temporal variation, watershed disturbanceAbstract
Introduction: Microplastics are now pervasive in aquatic ecosystems and represent a growing environmental concern. Their persistence and biological uptake threaten aquatic biodiversity and may affect food webs. In Costa Rica, research on microplastics has mainly focused on coastal and marine systems, leaving freshwater environments less explored. Museum specimens offer a unique opportunity to reconstruct historical patterns of microplastic contamination in these inland waters. Objectives: To examine temporal and spatial variation in microplastic ingestion in Astyanax aeneus, an abundant and widely distributed freshwater fish in Costa Rica. To (1) evaluate long-term changes, (2) compare basins with contrasting levels of human disturbance, and (3) test whether body size influences microplastic ingestion rates. Methods: We analyzed 180 specimens from a national collection spanning seven decades (1960–2025) across three river basins (San Carlos, Tárcoles and Térraba). Gastrointestinal tracts were digested with KOH and examined under stereomicroscopy to identify microplastic particles. Temporal and spatial variations were tested using ANOVAs, and relationships between fish size and microplastic load were assessed with correlation analyses. All procedures included strict contamination control. Results: All specimens contained microplastics, mainly fibers. Microplastic loads increased markedly over time in the San Carlos basin and were substantially higher in the heavily impacted Tárcoles basin. No relationship was detected between fish body size and the number of ingested particles. Conclusions: Freshwater fishes in tropical ecosystems are experiencing increasing microplastic exposure, primarily driven by watershed-level disturbance. The absence of a size-dependent pattern suggests that environmental availability and ecological context play a central role in shaping exposure. Moreover, this study supports the use of museum collections for retrospective monitoring of microplastic pollution and underscores the need for management strategies targeting plastic inputs and wastewater contamination.
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Agbekpornu, P., & Kevudo, I. (2023). The risks of microplastic pollution in the aquatic ecosystem. In E. S. Salama, & J. K. Summers (Eds.), Advances and challenges in microplastics (pp. 103–124). IntechOpen. https://doi.org/10.5772/intechopen.108717
Ahmad, M., Li, J. L., Wang, P. D., Hozzein, W. N., & Li, W. J. (2020). Environmental perspectives of microplastic pollution in the aquatic environment: A review. Marine Life Science & Technology, 2(4), 414–430. https://doi.org/10.1007/s42995-020-00056-w
Anderson, T. W., & Darling, D. A. (1952). Asymptotic theory of certain "goodness-of-fit" criteria based on stochastic processes. The Annals of Mathematical Statistics, 23(2), 193–212. https://doi.org/10.1214/aoms/1177729437
Angulo, A. (2021). New records and range extensions to the Costa Rican freshwater fish fauna, with an updated checklist. Zootaxa, 5083(1), 1–72. https://doi.org/10.11646/zootaxa.5083.1.1
Angulo, A., Bussing, W. A., López, M. I., Ramírez-Coghi, A. R., & Arias-Godínez, G. (2016). Colección ictiológica del Museo de Zoología de la Universidad de Costa Rica. In L. F. del Moral-Flores, A. J. Ramírez-Villalobos, J. A. Martínez-Pérez, A. F. González-Acosta, & J. Franco-López (Eds.), Colecciones ictiológicas de Latinoamérica (pp. 56–65). Universidad Nacional Autónoma de México.
Astorga, A., Montero-Cordero, A., Golfin-Duarte, G., García-Rojas, A., Vega-Bolaños, H., Arias-Zumbado, F., Solís-Adolio, D., & Ulate, K. (2022). Microplastics found in the world heritage site Cocos Island National Park, Costa Rica. Marine and Fishery Sciences, 35(3), 403–420. https://doi.org/10.47193/mafis.3532022010907
Astorga-Pérez, A., Ulate-Naranjo, K., & Abarca-Guerrero, L. (2022). Presencia de microplásticos en especies marinas del Parque Nacional Marino Las Baulas. Revista Tecnología en Marcha, 35(2), 27–38. https://doi.org/10.18845/tm.v35i2.5466
Azizi, N., Khoshnamvand, N., & Nasseri, S. (2021). The quantity and quality assessment of microplastics in the freshwater fishes: A systematic review and meta-analysis. Regional Studies in Marine Science, 47, 101955. https://doi.org/10.1016/j.rsma.2021.101955
Bermúdez-Guzmán, L., Alpízar-Villalobos, C., Gatgens-García, J., Jiménez-Huezo, G., Rodríguez-Arias, M., Molina, H., Villalobos, J., Paniagua, S. A., Vega-Baudrit, J. R., & Rojas-Jiménez, K. (2020). Microplastic ingestion by a herring Opisthonema sp. in the Pacific coast of Costa Rica. Regional Studies in Marine Science, 38, Article 101367. https://doi.org/10.1016/j.rsma.2020.101367
Boisen, O. C., Sidlauskas, B. L., Heppell, S. A., & Brander, S. M. (2024). Museum-archived myctophids reveal decadal trends in microplastic and microfiber ingestion. Science of the Total Environment, 954, 176310. https://doi.org/10.1016/j.scitotenv.2024.176310
Bussing, W. A. (1998). Freshwater fishes of Costa Rica (Vol. 46). Editorial Universidad de Costa Rica.
Conowall, P., Schreiner, K. M., Marchand, J., Minor, E. C., Schoenebeck, C. W., Maurer-Jones, M. A., & Hrabik, T. R. (2024). Variability in the drivers of microplastic consumption by fish across four lake ecosystems. Frontiers in Earth Science, 12, 1339822. https://doi.org/10.3389/feart.2024.1339822
Guillén-Watson, R., Arias-Andres, M., Rojas-Jiménez, K., & Wehrtmann, I. S. (2023). Microplastics in feed cause sublethal changes in the intestinal microbiota and a non-specific immune response indicator of the freshwater crayfish Procambarus clarkii (Decapoda: Cambaridae). Frontiers in Microbiology, 14, Article 1197312. https://doi.org/10.3389/fmicb.2023.1197312
Hammer, Ø., Harper, D. A. T., & Ryan, P. D. (2001). PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica, 4(1), 1–9.
Hauke, J., & Kossowski, T. (2011). Comparison of values of Pearson’s and Spearman’s correlation coefficients on the same sets of data. Quaestiones Geographicae, 30(2), 87–93. https://doi.org/10.2478/v10117-011-0021-1
Hermsen, E., Mintenig, S. M., Besseling, E., & Koelmans, A. A. (2018). Quality criteria for the analysis of microplastic in biota samples: A critical review. Environmental Science & Technology, 52(18), 10230–10240. https://doi.org/10.1021/acs.est.8b01611
Hinojosa-Garro, D., Rendón-von Osten, J., & Dzul-Caamal, R. (2020). Banded tetra (Astyanax aeneus) as bioindicator of trace metals in aquatic ecosystems of the Yucatan Peninsula, Mexico: Experimental biomarkers validation and wild populations biomonitoring. Ecotoxicology and Environmental Safety, 195, 110477. https://doi.org/10.1016/j.ecoenv.2020.110477
Holl, K. D., Daily, G. C., & Ehrlich, P. R. (1995). Knowledge and perceptions in Costa Rica regarding environment, population, and biodiversity issues. Conservation Biology, 9(6), 1548–1558. https://doi.org/10.1046/j.1523-1739.1995.09061548.x
Hou, L., McMahan, C. D., McNeish, R. E., Munno, K., Rochman, C. M., & Hoellein, T. J. (2021). A fish tale: A century of museum specimens reveal increasing microplastic concentrations in freshwater fish. Ecological Applications, 31(5), Article e02320. https://doi.org/10.1002/eap.2320
Ilechukwu, I., Das, R. R., & Reimer, J. D. (2023). Review of microplastics in museum specimens: An under-utilized tool to better understand the Plasticene. Marine Pollution Bulletin, 191, Article 114922. https://doi.org/10.1016/j.marpolbul.2023.114922
Kroes, A. D. A., & Finley, J. R. (2023). Demystifying omega-squared (ω²): Practical guidance for effect size in common ANOVA designs. Psychological Methods, 30(4), 866–887. https://doi.org/10.1037/met0000581
Lasdin, K. S., Arnold, M., Agrawal, A., Fennie, H. W., Grorud-Colvert, K., Sponaugle, S., Aylesworth, L., Heppell, S., & Brander, S. M. (2023). Presence of microplastics and microparticles in Oregon black rockfish sampled near marine reserve areas. PeerJ, 11, Article e14564. https://doi.org/10.7717/peerj.14564
Loureiro-Madureira, E. A., Carvalho da Silva, A. L., Barrantes-Castillo, G., & Vieira de Araújo, F. (2025). Occurrence and distribution of microplastics on the beaches of Limón on the Southern Caribbean Coast of Costa Rica. Micro, 5(1), 1. https://doi.org/10.3390/micro5010001
Lusher, A. L., Bråte, I. L. N., Munno, K., Hurley, R. R., & Welden, N. A. (2020). Is it or isn't it: The importance of visual classification in microplastic characterization. Applied Spectroscopy, 74(9), 1139–1153. https://doi.org/10.1177/0003702820930733
Modica, L., Lanuza, P., & García-Castrillo, G. (2020). Surrounded by microplastic, since when? Testing the feasibility of exploring past levels of plastic microfibre pollution using natural history museum collections. Marine Pollution Bulletin, 151, Article 110846. https://doi.org/10.1016/j.marpolbul.2019.110846
Monzó-López, M. (2022). Abundancia y distribución de microplásticos en el Pacífico y Caribe de Costa Rica [Trabajo de fin de grado, Universidad Católica de Valencia San Vicente Mártir]. Repositorio Institucional de la Universidad Católica de Valencia San Vicente Mártir. http://hdl.handle.net/20.500.12466/2567
Munno, K., Helm, P. A., Rochman, C., George, T., & Jackson, D. A. (2022). Microplastic contamination in Great Lakes fish. Conservation Biology, 36(1), Article e13794. https://doi.org/10.1111/cobi.13794
Okamoto, K., Nomura, M., Horie, Y., & Okamura, H. (2022). Color preferences and gastrointestinal-tract retention times of microplastics by freshwater and marine fishes. Environmental Pollution, 304, Article 119253. https://doi.org/10.1016/j.envpol.2022.119253
Pérez-Gómez, G., Alvarado-García, V., Rodríguez-Rodríguez, J. A., Herrera, F., & Sánchez-Gutiérrez, R. (2021). Calidad fisicoquímica y microbiológica del agua superficial del Río Grande de Tárcoles, Costa Rica: Un enfoque ecológico. UNED Research Journal, 13(1), Article e3148. https://doi.org/10.22458/urj.v13i1.3148
Portuguez, C. F., Mora Alvarado, D., & Brenes Salas, G. (2003). Calidad microbiológica del Río Grande de Térraba período 2002. Revista Costarricense de Salud Pública, 12(23), 11–21.
Rivas-Mena, G., Sánchez-Guerrero-Hernández, M. J., Yeste, M. P., Ramos, F., & González-Ortegón, E. (2024). Microplastics in the stomach content of the commercial fish species Scomber colias in the Gulf of Cadiz, SW Europe. Marine Pollution Bulletin, 200, 116049. https://doi.org/10.1016/j.marpolbul.2024.116049
Rodríguez-Barrientos, F. (2007). La microrregión como unidad espacial para el estudio de los problemas ambientales: Situación de los recursos suelo, forestal e hídrico en la microrregión Platanar-La Vieja, cuenca del río San Carlos. Tecnología en Marcha, 20(1), 62–79.
Rojas-Jiménez, K., Villalobos-Rojas, F., Gatgens-García, J., Rodríguez-Arias, M., Hernández-Montero, N., & Wehrtmann, I. S. (2022). Presence of microplastics in six bivalve species (Mollusca, Bivalvia) commercially exploited at the Pacific coast of Costa Rica, Central America. Marine Pollution Bulletin, 183, Article 114040. https://doi.org/10.1016/j.marpolbul.2022.114040
Sagot-Valverde, J. G. (2022). Monitoreo y caracterización de microplásticos en arenas de playas y aguas costeras de Costa Rica. Revista Internacional de Comunicación y Desarrollo, 4(17), 1–12. https://doi.org/10.15304/ricd.4.17.8807
Schröter, B., Castro-Arce, K., & Aguilar-González, B. J. (2023). Adjusted to reality? Mandate and functioning of the General Integral Commission of the Grande Tárcoles rivershed in Costa Rica. Ecology and Society, 28(4), Article 22. https://doi.org/10.5751/ES-14597-280422
Sequeira, I. F., Prata, J. C., da Costa, J. P., Duarte, A. C., & Rocha-Santos, T. (2020). Worldwide contamination of fish with microplastics: A brief global overview. Marine Pollution Bulletin, 160, Article 111681. https://doi.org/10.1016/j.marpolbul.2020.111681
Shapiro, S. S., & Wilk, M. B. (1965). An analysis of variance test for normality (complete samples). Biometrika, 52(3–4), 591–611. https://doi.org/10.1093/biomet/52.3-4.591
Sharma, S., & Chatterjee, S. (2017). Microplastic pollution, a threat to marine ecosystem and human health: A short review. Environmental Science and Pollution Research, 24, 21530–21547. https://doi.org/10.1007/s11356-017-9910-8
Small, G. E., Pringle, C. M., Pyron, M., & Duff, J. H. (2011). Role of the fish Astyanax aeneus (Characidae) as a keystone nutrient recycler in low-nutrient Neotropical streams. Ecology, 92(2), 386–397. https://doi.org/10.1890/10-0081.1
Sokal, R. R., & Rohlf, F. J. (2012). Biometry: The principles and practice of statistics in biological research (4th ed.). W. H. Freeman and Company.
Tukey, J. W. (1949). Comparing individual means in the analysis of variance. Biometrics, 5(2), 99–114. https://doi.org/10.2307/3001913
Umaña-Villalobos, G., & Springer, M. (2006). Variación ambiental en el Río Grande de Térraba y algunos de sus afluentes, Pacífico sur de Costa Rica. Revista de Biología Tropical, 54(Supl. 1), 265–272.
Vadera, S., & Khan, S. (2021). A critical analysis of the rising global demand of plastics and its adverse impact on environmental sustainability. Journal of Environmental Pollution and Management, 3(1), 1–13.
Welch, B. L. (1947). The generalization of 'Student's' problem when several different population variances are involved. Biometrika, 34(1–2), 28–35. https://doi.org/10.1093/biomet/34.1-2.28
Zazouli, M., Nejati, H., Hashempour, Y., Dehbandi, R., Nam, V. T., & Fakhri, Y. (2022). Occurrence of microplastics (MPs) in the gastrointestinal tract of fishes: A global systematic review and meta-analysis and meta-regression. Science of the Total Environment, 815, Article 152743. https://doi.org/10.1016/j.scitotenv.2021.152743
Zebral, Y. D., Pereira-Righi, B. D., Abou-Anni, I. S., Venquiaruti-Escarrone, A. L., Guillante, T., Delfino-Vieira, C. E., Gomes-Costa, P., & Bianchini, A. (2024). Organic contamination and multi-biomarker assessment in watersheds of the southern Brazil: An integrated approach using fish from the Astyanax genus. Environmental Science and Pollution Research, 31(21), 30543–30554. https://doi.org/10.1007/s11356-024-33181-5
Zúñiga-Umaña, J. M., Paniagua, S. A., Corrales-Brenes, R., & Vega-Baudrit, J. R. (2025). Microplastic pollution in Costa Rican marine ecosystems: Origins, ecotoxicological impacts, and mitigation strategies. Marine Policy, 179, Article 106772. https://doi.org/10.1016/j.marpol.2025.106772
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