Testing biodegradable mesh compositions to improve coral microfragmentation outcomes
DOI:
https://doi.org/10.15517/5bdh4q44Keywords:
coral growth; biodegradable substrates; restoration; technological innovation.Abstract
Introduction: Coral microfragmentation is a widely used technique in reef restoration, both in situ and in land-based nurseries. Typically, microfragments are individually attached to pedestals or artificial substrates, which requires substantial time and labor investment in the nursery and during field outplanting. An alternative approach involves the use of biodegradable meshes fixed to the substrate as a single unit supporting multiple microfragments. However, the optimal material combinations that provide the mechanical strength, stability, and adhesion necessary to maintain fragments in place after partial material degradation remain unknown. Therefore, experimental evaluation of biodegradable materials that optimize both stability and coral growth is a priority.
Objective: To evaluate whether two polymers (PCL and CAPA) combined with different concentrations of CaCO₃ influence coral growth rates and operational efficiency in both nursery and field conditions.
Methods: A controlled multifactorial experiment using a fully randomized block design was conducted over six months. The effect of mesh composition on cumulative growth in area and volume of Montastraea cavernosa was assessed using photogrammetry (Structure from Motion, SfM). Additionally, operational times in the nursery (handling, attachment, and cleaning) and during field outplanting were compared with and without mesh use.
Results: No statistically significant effects of mesh composition on growth rates were detected during the study period. Mean a real growth ranged from 0.5 to 0.8 mm², with a significant interaction among treatment, tank, and time, indicating low growth and high variability among tanks. Volumetric increase ranged from 0.25 to 0.62 cm³, with no significant differences among treatments. However, mesh uses reduced nursery handling time by approximately 20 minutes per rack of 18 fragments.
Conclusions: Regardless of composition, biodegradable meshes represent a viable alternative to optimize asexual propagation through microfragmentation, improving operational efficiency without compromising coral growth.
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Agudo-Adriani, E. A., Cappelletto, J., Cavada-Blanco, F., & Croquer, A. (2016). Colony geometry and structural complexity of the endangered species Acropora cervicornis partly explains the structure of their associated fish assemblage. PeerJ, 4, e1861. https://doi.org/10.7717/peerj.1861
Agudo-Adriani, E. A., Cappelletto, J., Cavada-Blanco, F., & Cróquer, A. (2019). Structural complexity and benthic cover explain reef-scale variability of fish assemblages in Los Roques National Park, Venezuela. Frontiers in Marine Science, 6, 690. https://doi.org/10.3389/fmars.2019.00690
Alvarez-Filip, L., Gill, J. A., Dulvy, N. K., Perry, A. L., Watkinson, A. R., & Côté, I. M. (2011). Drivers of region-wide declines in architectural complexity on Caribbean reefs. Coral reefs, 30(4), 1051–1060. https://doi.org/10.1007/s00338-011-0795-6
Anderson, M. J. (2005). PERMANOVA: a FORTRAN computer program for permutational multivariate analysis of variance. Department of Statistics, University of Auckland.
Anderson, M. (2008). PERMANOVA+ for PRIMER: guide to software and statistical methods (1st Ed.). Primer-E Limited.
Anderson, M. J., & Walsh, D. C. (2013). PERMANOVA, ANOSIM, and the Mantel test in the face of heterogeneous dispersions: what null hypothesis are you testing?. Ecological monographs, 83(4), 557–574. https://doi.org/10.1890/12-2010.1
Bayraktarov, E., Banaszak, A. T., Montoya Maya, P., Kleypas, J., Arias-González, J. E., Blanco M, Calle-Triviño, J., Charuvi, N., Cortés-Useche, C., Galván, V., García Salgado, M. A., Gnecco, M., Guendulain-García, S. D., Hernández Delgado, E. A., Marín Moraga, J. A., Maya, M. F., Mendoza Quiroz, S., Mercado Cervantes, S., Morikawa, M., … Frías-Torres, S. (2020). Coral reef restoration efforts in Latin American countries and territories. PLoS One, 15(8), e0228477. https://doi.org/10.1371/journal.pone.0228477
Borneman, E. (2008). Introduction to the husbandry of corals in aquariums: A review. In R. J. Leewis, & M. Janse (Eds.), Public Aquarium Husbandry Series (Vol. 2, pp. 3–14). Burgers’ Zoo.
Boström-Einarsson, L., Babcock, R. C., Bayraktarov, E., Ceccarelli, D., Cook, N., Ferse, S. C. A., Hancock, B., Harrison, P., Hein, M., Shaver, E., Smith, A., Suggett, D. J., Stewart-Sinclair, P., Vardi, T., & McLeod, I. M. (2020). Coral restoration-A systematic review of current methods, successes, failures and future directions. PLos ONE, 15, e0226631. https://doi.org/10.1371/journal.pone 0226631
Clarke, K. R., & Gorley, R. N. (2005). PRIMER: Getting started with v6. PRIMER-E Ltd:
Comba, D., Palmer, T. A., Breaux, N. J., & Pollack, J. B. (2023). Evaluating biodegradable alternatives to plastic mesh for small-scale oyster reef restoration. Restoration Ecology, 31(3), e13762. https://doi.org/10.1111/rec.13762
Crabbe, M. J. C. (2007). Global warming and coral reefs: Modelling the effect of temperature on Acropora palmata colony growth. Computational Biology and Chemistry, 31(4), 294–297. https://doi.org/10.1016/j.compbiolchem.2007.05.001
Croquer, A., Zambrano, S., Evangelista-Perez, D. Y., King, S., Guendulain-Garcia, S., Villalpando, M. F., & Sellares-Blasco, R. I. (2024). Short-term temporal trends of a coral reef in Samaná (Dominican Republic): The value of a permanent monitoring program to identify drivers of rapid change. Gulf and Caribbean Research, 35(1), 65–78. https://doi.org/10.18785/gcr.3501.16
Drury, C., Manzello, D., & Lirman, D. (2017). Genotype and local environment dynamically influence growth, disturbance response and survivorship in the threatened coral, Acropora cervicornis. PLoS One, 12(3), e0174000. https://doi.org/10.1371/journal.pone.0174000
Dullo, W. C. (2005). Coral growth and reef growth: a brief review. Facies, 51(1), 33–48. https://doi.org/10.1007/s10347-005-0060-y
Edwards, A., Guest, J., & Humanes, A. (2024). Rehabilitating coral reefs in the Anthropocene. Current Biology, 34(9), R399–R406.
Engler, L. G., Farias, N. C., Crespo, J. S., Gately, N. M., Major, I., Pezzoli, R., & Devine, D. M. (2023). Designing sustainable polymer blends: tailoring mechanical properties and degradation behaviour in PHB/PLA/PCL blends in a seawater environment. Polymers, 15, 2874. https://doi.org/10.3390/polym15132874
Enochs, I. C., Manzello, D. P., Carlton, R., Schopmeyer, S., van Hooidonk, R., & Lirman, D. (2014). Effects of light and elevated p CO2 on the growth and photochemical efficiency of Acropora cervicornis. Coral Reefs, 33(2), 477–485. https://doi.org/10.1007/s00338-014-1132-7
Forsman, Z. H., Rinkevich, B., & Hunter, C. L. (2006). Investigating fragment size for culturing reef-building corals (Porites lobata and P. compressa) in ex situ nurseries. Aquaculture, 261(1), 89–97. https://doi.org/10.1016/j.aquaculture.2006.06.040
Forsman, Z. H., Page, C. A., Toonen, R. J., & Vaughan, D. (2015). Growing coral larger and faster: micro-colony-fusion as a strategy for accelerating coral cover. PeerJ, 3, e1313. https://doi.org/10.7717/peerj.1313
Foo, S. A., & Asner, G. P. (2019). Scaling up coral reef restoration using remote sensing technology. Frontiers in Marine Science, 6, 79. https://doi.org/10.3389/fmars.2019.00079
Gomez, E., Dizon, R., & Edwards, A. (2010). Methods of coral transplantation. In A. J. Edwards (Ed.), Reef Rehabilitation Manual (pp. 99–112). Coral Reef Targeted Research & Capacity Building for Management Program
Gutierrez, L., Polidoro, B., Obura, D., Cabada-Blanco, F., Linardich, C., Pettersson, E., Alvarado, J. J., Alvarez-Filip, L., Banaszak, A., Casado de Amezua, P., Crabbe, J., Croquer, A., Feingold, J., Goergen, E., Goffredo, S., Hoeksema, B., Huang, D., & Wilson, B. (2024). Half of Atlantic reef-building corals at elevated risk of extinction due to climate change and other threats. PloS one, 19(11), e0309354. https://doi.org/10.1371/journal.pone.0309354
Gutiérrez-Estrada, G., Tortolero-Langarica, J. A., & Carricart-Ganivet, J. P. (2025). Modelling coral calcification rates in Orbicella faveolata (Cnidaria: Scleractinia) using light attenuation coefficients in water (KdPAR). Marine Environmental Research, 207, 107074. https://doi.org/10.1016/j.marenvres.2025.107074
JinJiang Shengjin Technology CO., LTD. (2023). Polycaprolactone. Shengjin Technology. http://www.jjshengjin.com/product_detail_en/id/16.html
Jones, A., & Berkelmans, R. (2010). Potential costs of acclimatization to a warmer climate: growth of a reef coral with heat tolerant vs. sensitive symbiont types. PloS One, 5(5), e10437. https://doi.org/10.1371/journal.pone.0010437
Kenyon, T. M., Jones, C., Rissik, D., Brassil, W., Callaghan, D., Mattocks, N., & Baldock, T. E. (2025). Bio-degradable ‘reef bags’ used for rubble stabilisation and their impact on rubble stability, binding, coral recruitment and fish occupancy. Ecological Engineering, 210, 107433. https://doi.org/10.1016/j.ecoleng.2024.107433
Knapp, I. S., Forsman, Z. H., Greene, A., Johnston, E. C., Bardin, C. E., Chan, N., Wolke, C., Gulko, D., & Toonen, R. J. (2022). Coral micro-fragmentation assays for optimizing active reef restoration efforts. PeerJ, 10, e13653. https://doi.org/10.7717/peerj.13653
Koch, H. R., Wallace, B., DeMerlis, A., Clark, A. S., & Nowicki, R. J. (2021). 3D scanning as a tool to measure growth rates of live coral microfragments used for coral reef restoration. Frontiers in Marine Science, 8, 623645. https://doi.org/10.3389/fmars.2021.623645
Ladd, M. C., Shantz, A. A., Bartels, E., & Burkepile, D. E. (2017). Thermal stress reveals a genotype-specific tradeoff between growth and tissue loss in restored Acropora cervicornis. Marine Ecology Progress Series, 572, 129–139. https://doi.org/10.3354/meps12169
Leonard, C., Hédouin, L., Lacorne, M. C., Dalle, J., Lapinski, M., Blanc, P., & Nugues, M. M. (2022). Performance of innovative materials as recruitment substrates for coral restoration. Restoration Ecology, 30(7), e13625. https://doi.org/10.1111/rec.13625
Lirman, D., Schopmeyer, S., Galvan, V., Drury, C., Baker, A. C., & Baums, I. B. (2014). Growth dynamics of the threatened Caribbean staghorn coral Acropora cervicornis: influence of host genotype, symbiont identity, colony size, and environmental setting. PLoS one, 9(9), e107253. https://doi.org/10.1371/journal.pone.0107253
Lott, C., Eich, A., Unger, B., Makarow, D., Battagliarin, G., Schlegel, K., Lasut, M. T., & Weber, M. (2020). Field and mesocosm methods to test biodegradable plastic film under marine conditions. PLoS One, 15(7), e0236579. https://doi.org/10.1371/journal.pone.0236579
Martin-Garin, B., & Montaggioni, L. F. (2023). Coral Reefs in the Face of Their Fate. In B. Martin-Garin, & L. F. Montaggioni (Eds.), Corals and Reefs: From the Beginning to an Uncertain Future (Vol. 16, pp. 145–158). Springer. https://doi.org/10.1007/978-3-031-16887-1_6
Mavromatis, V., Brazier, J. M., & Goetschl, K. E. (2022). Controls of temperature and mineral growth rate on Mg incorporation in aragonite. Geochimica et Cosmochimica Acta, 317, 53–64. https://doi.org/10.1016/j.gca.2021.10.015
Mies, M., Longo, G. O., Bianchini, A., Calderon, E. N., Castro, C. B., Faria, S. C., Francini-Filho, R. B., Guebert, F. M., Kitahara, M. V., Lacerda, C. H. F., Lotufo, T. M. C., Marangoni, L. F. B., Pires, D. O., & Cordeiro, R. T. S. (2025). Challenges for coral restoration in Southwestern Atlantic reefs: guidelines for ethical and sustainable practices. Biodiversity and Conservation, 1–27. https://doi.org/10.1007/s10531-025-03071-4
Mostrales, T. P. I., Rollon, R. N., & Licuanan, W. Y. (2022). Evaluation of the performance and cost-effectiveness of coral microfragments in covering artificial habitats. Ecological Engineering, 184, 106770. https://doi.org/10.1016/j.ecoleng.2022.106770
Mulà, C., Bradshaw, C. J., Cabeza, M., Manca, F., Montano, S., & Strona, G. (2025). Restoration cannot be scaled up globally to save reefs from loss and degradation. Nature Ecology & Evolution, 9, 822–832. https://doi.org/10.1038/s41559-025-02667-x
Page, C. A., Muller, E. M., & Vaughan, D. E. (2018). Microfragmenting for the successful restoration of slow growing massive corals. Ecological Engineering, 123, 86–94. https://doi.org/10.1016/j.ecoleng.2018.08.017
Page, C., Perry, R., Lager, C. V., Daly, J., Bouwmeester, J., Henley, E. M., & Hagedorn, M. (2023). Tank fouling community enhances coral microfragment growth. PeerJ, 11, e15723. https://doi.org/10.7717/peerj.15723
Pandolfi, J. M., Bradbury, R. H., Sala, E., Hughes, T. P., Bjorndal, K. A., Cooke, R. G., McArdle, D., McClenachan, L., Newman, M. J. H., Paredes, G., Warner, R. R., & Jackson, B. C. (2003). Global trajectories of the long-term decline of coral reef ecosystems. Science, 301(5635), 955–958. https://doi.org/10.1126/science.1085706
Perry, C. T., & Alvarez-Filip, L. (2019). Changing geo-ecological functions of coral reefs in the Anthropocene. Functional Ecology, 33(6), 976–988. https://doi.org/10.1111/1365-2435.13247
Perstorp Holding AB. (nd). CAPA ™ 6800. Polycaprolactone. [Technical information]. https://www.sushengpolymer.com/media/pdf/CM1qmR_Capa-6800.pdf
Pratchett, M. S., Anderson, K. D., Hoogenboom, M. O., Widman, E., Baird, A. H., Pandolfi, J. M., Edmunds, P. J., & Lough, J. M. (2015). Spatial, temporal and taxonomic variation in coral growth—implications for the structure and function of coral reef ecosystems. In R. N. Hughes, D. J. Hughes, & A. C. Dale (Eds.), Oceanography and Marine Biology: An annual review (Vol. 53, pp. 215–295). CRC Press. https://doi.org/10.1201/b18733
Randall, C. J., & Szmant, A. M. (2009). Elevated temperature affects development, survivorship, and settlement of the elkhorn coral, Acropora palmata (Lamarck 1816). The Biological Bulletin, 217(3), 269–282. https://doi.org/10.1086/BBLv217n3p269
Rinkevich, B. (2005). Conservation of coral reefs through active restoration measures: recent approaches and last decade progress. Environmental Science & Technology, 39(12), 4333–4342. https://doi.org/10.1021/es0482583
Reverter, M., Helber, S. B., Rohde, S., de Goeij, J. M., & Schupp, P. J. (2024). Drivers of coral reef benthic changes and implications on ecosystem functioning and services. In P. A. Todd, & B. D. Russell (Eds.), Oceanography and Marine Biology (Vol. 62, pp. 215–247). CRC Press. https://doi.org/10.1201/9781003477518
Rivera, A., Ceballos, M., Macdonald, S., Veras, D., León-Zubillaga, A., Aponte-Guillén, M., Cruz, L., García-Camps, R., & Croquer, A. (2023, May 22–26). Optimization of a land-based nursery: New approaches to maximize performance in Punta Cana, Dominican Republic [Poster presentation]. 40th AMLC Scientific Meeting, St. Kitts.
Schill, S. R., Asner, G. P., McNulty, V. P., Pollock, F. J., Croquer, A., Vaughn, N. R., Escobar-Fadul, X., Raber, G., Shaver, E., & Shaver, E. (2021). Site selection for coral reef restoration using airborne imaging spectroscopy. Frontiers in Marine Science, 8, 698004. https://doi.org/10.3389/fmars.2021.698004
Schmidt-Roach, S., Knorr, T., Roch, C., Klaus, R., Klepac, C., Klein, S. G., & Duarte, C. M. (2025). Cost-efficiency and effectiveness of coral restoration pathways. Restoration Ecology, 33(1), e14326. https://doi.org/10.1111/rec.14326
Sellares-Blasco, R. I., Villalpando, M. F., Guendulain-García, S. D., & Croquer, A. (2021). Assisted coral reproduction in the Dominican Republic: a successful story to replicate in the Caribbean. Frontiers in Marine Science, 8, 669505. https://doi.org/10.3389/fmars.2021.669505
Special Chem (2026). Plastics and elastomers supplied by Ingevity: Capa. https://www.specialchem.com/plastics/supplier/ingevity/capa
Strudwick, P., Camp, E. F., Seymour, J., Roper, C., Edmondson, J., Howlett, L., & Suggett, D. J. (29 March, 2023). Assessing efficacy of plastic-free alternative ties for coral propagation in reef restoration. (PREPRINT, Version 1). Research Square. https://doi.org/10.21203/rs.3.rs-2729419/v1
Strudwick, P., Camp, E. F., Seymour, J., Roper, C., Edmondson, J., Howlett, L., & Suggett, D. J. (2024). Impacts of plastic-free materials on coral-associated bacterial communities during restoration. Environmental Microbiology Reports, 16(1), e13229. https://doi.org/10.1111/1758-2229.13229
Suggett, D. J., Edwards, M., Cotton, D., Hein, M., & Camp, E. F. (2023). An integrative framework for sustainable coral reef restoration. One Earth, 6(6), 666–681. https://doi.org/10.1016/j.oneear.2023.05.007
Suzuki, M., Tachibana, Y., & Kasuya, K. I. (2021). Biodegradability of poly (3-hydroxyalkanoate) and poly (ε-caprolactone) via biological carbon cycles in marine environments. Polymer Journal, 53, 47–66. https://doi.org/10.1038/s41428-020-00396-5
TRiiSO (2018). Physical properties of CAPA ™ thermoplastics. Leaflet TI 1658. [Technical information]. Perstorp. Winning Formula. https://www.tri-iso.com/documents/Physical_Properties_of%20Capa_Thermoplastic_Resins.pdf
TRiiSO (2019). Capa® 6800 [Technical information]. Ingevity https://www.tri-iso.com/documents/Ingevity_CAPA_6800_TDS.pdf
Vardi, T., Hoot, W. C., Levy, J., Shaver, E., Winters, R. S., Banaszak, A. T., Baums, I. B., Chamberland, V. F., Cook, N., Gulko, D., Hein, M. Y., Kaufman, L., Loewe, M., Lundgren, P., Lustic, C., MacGowan, P., Marz, M. V., MCGonigle, M., McLeod, I., ... Montoya-Maya, P. H. (2021). Six priorities to advance the science and practice of coral reef restoration worldwide. Restoration Ecology, 29(8), e13498. https://doi.org/10.1111/rec.13498
Walters, L. J., Roddenberry, A., Crandall, C., Wayles, J., Donnelly, M., Barry, S. C., Clark, S. C., Escandell, O., Hansen, J. C., Laakkonen, K., & Sacks, P. E. (2022). The use of non-plastic materials for oyster reef and shoreline restoration: understanding what is needed and where the field is headed. Sustainability, 14(13), 8055. https://doi.org/10.3390/su14138055
Wang, G. X., Huang, D., Ji, J. H., Völker, C., & Wurm, F. R. (2021). Seawater-degradable polymers—Fighting the marine plastic pollution. Advanced Science, 8(1), 2001121. https://doi.org/10.1002/advs.202001121
Williams, G. J., Graham, N. A., Jouffray, J. B., Norström, A. V., Nyström, M., Gove, J. M., Heenan, A., & Wedding, L. M. (2019). Coral reef ecology in the Anthropocene. Functional Ecology, 33(6), 1014–1022. https://doi.org/10.1111/1365-2435.13290
Yap, H. T., & Alvarez Molina, R. (2003). Comparison of coral growth and survival under enclosed, semi-natural conditions and in the field. Marine Pollution Bulletin, 46(7), 858–864. https://doi.org/10.1016/S0025-326X(03)00064-X
Young, C. N., Schopmeyer, S. A., & Lirman, D. (2012). A review of reef restoration and coral propagation using the threatened genus Acropora in the Caribbean and Western Atlantic. Bulletin of Marine Science, 88(4), 1075–1098. https://doi.org/10.5343/bms.2011.1143
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