Effects of Aloe vera Gel on Osteoclast and Osteoblast Activity and Receptor Activator of Nuclear Factor Kappa-B Ligand Expression During Bone Remodeling Following Orthodontic Temporary Anchorage Device Placement: An In Vivo Study

Siregar, Pepi Riyani; Ranggang, Baharuddin M.; Hidayati, Nasyrah; Habar, Eddy Heriyanto; Erwansyah, Eka; Pawinru, Ardiansyah S.; Paramma, Zilal Islamy

doi:10.15517/nwaze102

Authors

Pepi Riyani Siregar Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Author https://orcid.org/0009-0000-3594-7884
Baharuddin M. Ranggang Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Author https://orcid.org/0009-0002-6573-8922
Nasyrah Hidayati Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Author https://orcid.org/0009-0001-3488-7952
Eddy Heriyanto Habar Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Author https://orcid.org/0000-0002-4228-0624
Eka Erwansyah Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Author https://orcid.org/0000-0002-1760-2409
Ardiansyah S. Pawinru Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Author https://orcid.org/0000-0002-4763-5439
Zilal Islamy Paramma Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Author https://orcid.org/0009-0003-2089-5235

DOI:

https://doi.org/10.15517/nwaze102

Keywords:

Aloe vera; Bone remodeling; Osteoblasts; Osteoclasts; RANKL; Temporary Anchorage Device (TAD).

Abstract

The biomedical use of Aloe vera continues to expand, including in orthodontics where its role in bone remodeling is of particular interest. This study aimed to evaluate the effect of Aloe vera gel on osteoclast, osteoblast, and RANKL expression following Temporary Anchorage Device (TAD) placement. Thirty male New Zealand White rabbits were randomly assigned to control and treatment groups. TADs were placed in the maxillary buccal region, and the treatment group received 50% Aloe vera gel topically. Each rabbit in the treatment group received 0.5 g of 50% Aloe vera gel topically around the TAD site twice daily. Histological analysis and ELISA were conducted on days 3, 7, and 14. The results showed a significant increase in osteoblast counts on days 7 and 14 (p<0.05). Osteoclast counts remained elevated on day 3 but were significantly lower than the control group (p < 0.05), followed by a further decrease on day 14. RANKL expression increased moderately over time (R²=0.517). Regression analysis demonstrated a time-dependent effect of Aloe vera on osteoblasts (R²=0.834) and osteoclasts (R²=0.928). These findings suggest that Aloe vera gel supports bone remodeling by relatively suppressing osteoclast activity, enhancing osteoblast response, and modulating RANKL expression proportionally, making it a promising adjunct for improving TAD stability in clinical orthodontics.

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Author Biographies

Pepi Riyani Siregar, Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.

Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Indonesia.
Baharuddin M. Ranggang, Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.

Assistant Professor, Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Indonesia.
Nasyrah Hidayati, Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.

Lecturer Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Indonesia.
Eddy Heriyanto Habar, Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.

Associate Professor, Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.
Eka Erwansyah, Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.

Associate Professor, Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.
Ardiansyah S. Pawinru, Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.

Associate Professor, Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.
Zilal Islamy Paramma, Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.

Lecturer Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Indonesia.

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