Effects of Aloe vera Gel on Osteoclast and Osteoblast Activity and Receptor Activator of Nuclear Factor Kappa-B Ligand Expression During Bone Remodeling Following Orthodontic Temporary Anchorage Device Placement: An In Vivo Study

Siregar, Pepi Riyani; Ranggang, Baharuddin M.; Hidayati, Nasyrah; Habar, Eddy Heriyanto; Erwansyah, Eka; Pawinru, Ardiansyah S.; Paramma, Zilal Islamy

doi:10.15517/nwaze102

Autores/as

Pepi Riyani Siregar Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Autor/a https://orcid.org/0009-0000-3594-7884
Baharuddin M Ranggang Hasanuddin university Autor/a https://orcid.org/0009-0002-6573-8922
Nasyrah Hidayati Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Autor/a https://orcid.org/0009-0001-3488-7952
Eddy Heriyanto Habar Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Autor/a https://orcid.org/0000-0002-4228-0624
Eka Erwansyah Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Autor/a https://orcid.org/0000-0002-1760-2409
Ardiansyah S. Pawinru Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Autor/a https://orcid.org/0000-0002-4763-5439
Zilal Islamy Paramma Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Autor/a https://orcid.org/0009-0003-2089-5235

DOI:

https://doi.org/10.15517/nwaze102

Palabras clave:

Aloe vera; Remodelación ósea; Osteoblastos; Osteoclastos; RANKL; Dispositivo de anclaje temporal (TAD).

Resumen

El uso biomédico del Aloe vera continúa expandiéndose, incluido su empleo en ortodoncia, donde su papel en la remodelación ósea resulta de especial interés. Este estudio tuvo como objetivo evaluar el efecto del gel de Aloe vera sobre la actividad de osteoclastos, osteoblastos y la expresión de RANKL tras la colocación de dispositivos de anclaje temporal (TAD, por sus siglas en inglés). Treinta conejos machos New Zealand fueron asignados aleatoriamente a grupos control y tratamiento. Los TAD se colocaron en la región bucal maxilar, y el grupo de tratamiento recibió gel de Aloe vera al 50% de forma tópica. Cada conejo del grupo tratado recibió 0.5 g de gel de Aloe vera al 50% aplicado tópicamente alrededor del sitio del TAD dos veces al día. Se realizaron análisis histológicos y ELISA en los días 3, 7 y 14. Los resultados mostraron un aumento significativo en el recuento de osteoblastos en los días 7 y 14 (p<0.05). El recuento de osteoclastos se mantuvo elevado en el día 3, pero fue significativamente menor que en el grupo control (p<0.05), seguido de una disminución adicional en el día 14. La expresión de RANKL aumentó moderadamente con el tiempo (R²=0.517). El análisis de regresión mostró un efecto dependiente del tiempo del Aloe vera sobre los osteoblastos (R²=0.834) y los osteoclastos (R²=0.928). Estos hallazgos sugieren que el gel de Aloe vera favorece la remodelación ósea al suprimir relativamente la actividad osteoclástica, potenciar la respuesta osteoblástica y modular proporcionalmente la expresión de RANKL, lo que lo convierte en un coadyuvante prometedor para mejorar la estabilidad de los TAD en la práctica clínica ortodóntica.

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Biografía del autor/a

Pepi Riyani Siregar, Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.

Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Indonesia.
Baharuddin M Ranggang, Hasanuddin university

Assistant Professor, Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Indonesia.
Nasyrah Hidayati, Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.

Lecturer Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Indonesia.
Eddy Heriyanto Habar, Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.

Associate Professor, Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.
Eka Erwansyah, Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.

Associate Professor, Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.
Ardiansyah S. Pawinru, Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.

Associate Professor, Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.
Zilal Islamy Paramma, Department of Orthodontic. Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.

Lecturer Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Indonesia.

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