GO-Sr-Se Multi-Doped Nanohydroxyapatite Synergistically Promotes BMSCs Osteogenic Differentiation, and Antibacterial Efficacy: In Vitro Study
DOI:
https://doi.org/10.15517/4d4ve788Keywords:
Hydroxyapatite; Nanoparticles; Strontium; Selenium; Bone marrow; Stem cells.Abstract
Nanohydroxyapatite (nHA) is one of the most widely used bone-substitution materials in biomedical applications. Some clinical scenarios involving extensive bone loss and bony infections may require further development of therapeutic modalities to enhance material characteristics. Doping nHA with some metal ions can improve its physical and biological properties and address its limitations. Therefore, the ongoing study aims to examine the role of doped nanohydroxyapatite with graphene oxide (GO), strontium (Sr), and selenium (Se) ions (GO-Sr-Se-doped nHA) formulation as a potential bone substitution material on bone marrow stem cells (BMSCs). Within this study, nHA and GO-Sr-Se-doped nHA were synthesized by the microwave-assisted sol-gel technique. BMSCs were isolated from rats and treated with these two types of nanoparticles. Their effect on BMSCs’ osteogenic potential was evaluated by alkaline phosphatase (ALP) activity, and calcium mineralization was assessed by Alizarin Red S (ARS). Antibacterial activity against both Staphylococcus aureus and Escherichia coli was also assessed through the agar diffusion method. GO-Sr-Se-nHA supplementation of the culture medium significantly increased both ALP enzyme production (P<0.05) and calcium mineral deposition (P<0.05) and significantly inhibited the growth of both Escherichia coli and Staphylococcus aureus (P<0.05). Our results indicated that GO-Sr-Se-nHA doped nanoparticles could enhance BMSCs osteogenic differentiation, with potent antibacterial activity if compared to plain nHA, making them a suitable candidate for treating bone defects and their use in future bone regenerative applications.
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