Nanohidroxiapatita multidopada con GO-Sr-Se promueve sinérgicamente la diferenciación osteogénica de BMSC y la eficacia antibacteriana: estudio in vitro
DOI:
https://doi.org/10.15517/4d4ve788Palabras clave:
Hidroxiapatita; Nanopartículas; Estroncio; Selenio; Médula ósea; Células madre.Resumen
La nanohidroxiapatita (nHA) es uno de los materiales de sustitución ósea más utilizados en aplicaciones biomédicas. Algunos escenarios clínicos que implican pérdida ósea extensa e infecciones óseas pueden requerir el desarrollo de modalidades terapéuticas adicionales para mejorar las características del material. El dopaje de la nHA con ciertos iones metálicos puede mejorar sus propiedades físicas y biológicas y abordar sus limitaciones. Por lo tanto, el presente estudio tiene como objetivo examinar el papel de la formulación de nanohidroxiapatita dopada con óxido de grafeno (GO), estroncio (Sr) y selenio (Se) (nHA dopada con GO-Sr-Se) como un posible material de sustitución ósea sobre células madre de médula ósea (BMSC, por sus siglas en inglés). En este estudio, la nHA y la nHA dopada con GO-Sr-Se se sintetizaron mediante la técnica sol-gel asistida por microondas. Las BMSC se aislaron de ratas y se trataron con estos dos tipos de nanopartículas. Su efecto sobre el potencial osteogénico de las BMSC se evaluó mediante la actividad de la fosfatasa alcalina (ALP), y la mineralización de calcio se evaluó mediante Rojo de Alizarina S (ARS). También se evaluó la actividad antibacteriana frente a Staphylococcus aureus y Escherichia coli mediante el método de difusión en agar. La suplementación del medio de cultivo con nHA-GO-Sr-Se aumentó significativamente tanto la producción de la enzima ALP (P<0.05) como el depósito mineral de calcio (P<0.05), e inhibió significativamente el crecimiento de Escherichia coli y Staphylococcus aureus (P<0.05). Nuestros resultados indicaron que las nanopartículas dopadas con nHA-GO-Sr-Se podrían mejorar la diferenciación osteogénica de las BMSC, con una potente actividad antibacteriana en comparación con la nHA simple, lo que las convierte en un candidato adecuado para el tratamiento de defectos óseos y su uso en futuras aplicaciones de regeneración ósea.
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Derechos de autor 2026 Mariam Salah Ibrahim Hassan, Galal ElDeen Mosaad Sadek, Ahmed Wael Abouzeid, Radwa Hamed Hegazy, Sara El Moshy.
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