Biocompatibility and Elemental Characterization of Bioceramic Root Canal Sealers vs Resin Sealer AH Plus
DOI:
https://doi.org/10.15517/y9jph839Keywords:
Root canal sealers; Biocompatibility; Human gingival fibroblasts; Characterization.Abstract
Root canal sealers must be biocompatible with periapical tissues and exhibit adequate physicochemical properties. This study aimed to characterize and evaluate the biocompatibility of two commercially available bioceramic root canal sealers (NeoSEALER Flo and AH Plus Bioceramic Sealer) and one epoxy-amine resin–based sealer (AH Plus). Surface morphology was analyzed by scanning electron microscopy (SEM), chemical composition by Fourier Transform Infrared Spectroscopy (FTIR), and radiopacity according to ISO 6876 specifications. Biocompatibility was assessed using human gingival fibroblasts (hGFs) through WST-1 cell proliferation assay after 24 and 48 h. All sealers demonstrated adequate cytocompatibility, with no cytotoxic effects observed. AH Plus showed significantly higher cell proliferation at 24 h, while AH Plus Bioceramic exhibited increased proliferation at 48 h. SEM analysis revealed distinct surface topographies among materials, with AH Plus presenting a more homogeneous surface and bioceramic sealers showing granular and porous structures. FTIR confirmed the characteristic chemical composition of each material. All sealers met ISO radiopacity requirements, with AH Plus exhibiting the highest radiopacity. All evaluated sealers demonstrated suitable biological and physicochemical properties.
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