Biocompatibilidad y caracterización elemental de selladores endodónticos biocerámicos vs sellador de resina AH Plus
DOI:
https://doi.org/10.15517/y9jph839Palabras clave:
Selladores de conductos radiculares; Biocompatibilidad; Fibroblastos gingivales humanos; Caracterización.Resumen
Los selladores de conductos radiculares deben ser biocompatibles con los tejidos periapicales y presentar propiedades fisicoquímicas adecuadas. Este estudio tuvo como objetivo caracterizar y evaluar la biocompatibilidad de dos selladores biocerámicos para conductos radiculares disponibles comercialmente (NeoSEALER Flo y AH Plus Bioceramic Sealer) y un sellador a base de resina epoxi-amina (AH Plus). La morfología superficial se analizó mediante microscopía electrónica de barrido (SEM), la composición química mediante Espectroscopía de Infrarrojos con Transformada de Fourier (FTIR) y la radiopacidad de acuerdo con las especificaciones de la norma ISO 6876. La biocompatibilidad se evaluó utilizando fibroblastos gingivales humanos (hGFs) mediante el ensayo de proliferación celular WST-1 después de 24 y 48 horas. Todos los selladores demostraron una citocompatibilidad adecuada, sin observarse efectos citotóxicos. AH Plus mostró una proliferación celular significativamente mayor a las 24 horas, mientras que AH Plus Bioceramic presentó un aumento en la proliferación a las 48 horas. El análisis mediante SEM reveló topografías superficiales distintas entre los materiales, con AH Plus presentando una superficie más homogénea y los selladores biocerámicos mostrando estructuras granulares y porosas. El FTIR confirmó la composición química característica de cada material. Todos los selladores cumplieron con los requisitos de radiopacidad de la norma ISO, siendo AH Plus el que presentó la mayor radiopacidad. En conjunto, todos los selladores evaluados demostraron propiedades biológicas y fisicoquímicas adecuadas.
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Derechos de autor 2026 Eduardo Adrián Sánchez Barajas, Alejandra Rodríguez Hidalgo, Marine Ortiz Magdaleno, Rafael Álvarez-Chimal, Amaury Pozos-Guillén, Febe Carolina Vázquez Vázquez.
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