El inhibidor de la histona desacetilasa butirato de sodio estimula la adipogénesis a través de un cambio transcripcional limitado en células troncales derivadas de ligamento periodontal
Palabras clave:
Células troncales de ligamento periodontal; Acetilación de histonas; Adipogénesis; Inhibidores de histonas desacetilasas.Resumen
La diferenciación adipogénica desempeña un papel crucial en la biología del tejido adiposo, un órgano endocrino que regula el almacenamiento de energía y la secreción hormonal. La disfunción en este proceso contribuye a enfermedades metabólicas como la obesidad y la diabetes tipo II. Se han desarrollado modelos in vitro para investigar los mecanismos de la adipogénesis, y las células troncales de ligamento periodontal (CTLP) emergen como un modelo prometedor debido a su capacidad multipotente. Estudios previos han demostrado que la manipulación epigenética puede mejorar la respuesta adipogénica en varias líneas celulares. La acetilación de la lisina 9 en la histona H3 (H3K9ac) se asocia con la activación de genes clave, como PPARγ-2. En este estudio, evaluamos si los inhibidores de la histona desacetilasa de clase I, como el ácido valproico (VPA) y el butirato de sodio (NaBu), ambos ácidos grasos de cadena corta, pueden aumentar la acetilación de H3K9 e influir en la diferenciación adipogénica. Utilizamos concentraciones de VPA de 1, 4 y 8 mM y NaBu de 1, 2 y 5 mM para evaluar sus efectos sobre la viabilidad celular, la morfología, la distribución de H3K9ac y la diferenciación adipogénica. Los resultados indicaron que las células tratadas con NaBu de 5 mM presentaron cambios morfológicos, menor viabilidad, mayor intensidad de la señal de H3K9ac y mayor deposición lipídica intracelular. Estos resultados sugieren que la inhibición de las HDAC por NaBu aumenta la plasticidad hacia la adipogénesis de las CTLP mediante un cambio transcripcional limitado en sus genes clave.
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Derechos de autor 2025 Anahí Torres-Nájera, Angélica Anahí Serralta-Interían, Rodrigo Arturo Rivera-Solís, Geovanny Nic-Can, Leydi Carrillo-Cocom, Beatriz Adriana Rodas-Junco.
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