Evaluation of Colonization and Biofilm Formation of E. faecalis over G-MTA, W-MTA and Biodentine. In vitro study

Ruiz-Flores, Alejandra; González-Amaro, Ana Maria; Zavala-Alonso, Norma Verónica; Gutiérrez-Cantú, Francisco Javier; Mariel-Cárdenas, Jairo; Oliva-Rodríguez, Ricardo; Muñoz-Ruiz, Abraham I.

doi:10.15517/v30z1132

Autores/as

Alejandra Ruiz-Flores Postgraduate Dental Science Program, Faculty of Dentistry, UASLP, San Luis Potosí, SLP, México. Autor/a https://orcid.org/0009-0004-1442-5031
Ana Maria González-Amaro Endodontics Postgraduate Program, Faculty of Dentistry, UASLP, San Luis Potosí, SLP, México Autor/a https://orcid.org/0000-0002-6375-9642
Norma Verónica Zavala-Alonso Postgraduate Dental Science Program, Faculty of Dentistry, UASLP, San Luis Potosí, SLP, México. Autor/a https://orcid.org/0000-0001-8822-185X
Francisco Javier Gutiérrez-Cantú Department of Morphology, Faculty of Dentistry, UASLP, San Luis Potosí, SLP, México. Autor/a https://orcid.org/0000-0001-7220-5791
Jairo Mariel-Cárdenas Department of Morphology, Faculty of Dentistry, UASLP, San Luis Potosí, SLP, México. Autor/a https://orcid.org/0000-0002-4733-7271
Ricardo Oliva-Rodríguez Endodontics Postgraduate Program, Faculty of Dentistry, UASLP, San Luis Potosí, SLP, México. Autor/a https://orcid.org/0000-0002-1797-1773
Abraham I. Muñoz-Ruiz Postgraduate Dental Science Program, Faculty of Dentistry, UASLP, San Luis Potosí, SLP, México. Autor/a https://orcid.org/0000-0003-2522-3617

DOI:

https://doi.org/10.15517/v30z1132

Palabras clave:

Apicectomía, Material de retro-obturación, Biodentine, MTA-Gris, MTA-Blanco.

Resumen

La apicectomía es un procedimiento indicado para el tratamiento de lesiones y periodontitis apical persistente después de un tratamiento de conductos; el procedimiento consiste en la eliminación de los últimos 3 mm apicales y posterior creación de una preparación para finalmente sellarlo con un material de obturación. Se ha reportado que los materiales biocerámicos (MTA-Gris, MTA-Blanco y Biodentine) tienen propiedades antibacterianas adecuadas. El objetivo de este estudio es evaluar la capacidad de colonización y formación de biofilm de E. faecalis sobre la superficie de materiales biocerámicos in vitro. Se emplearon 180 discos de dentina aleatorizados en 3 grupos experimentales (G-MTA+E. faecalis, W-MTA E. faecalis y Biodentine+E. faecalis) y 3 grupos control (G-MTA, W-MTA y Biodentine), El área/superficie cubierta por biofilm y microorganismos fue evaluada mediante SEM. Todas las muestras de los grupos experimentales mostraron una superficie rugosa e irregular debido a la colonización de microorganismos y la formación de biopelículas. El grupo de MTA-Gris mostró mejores propiedades antimicrobianas que MTA-Blanco y Biodentine. La eliminación y extirpación completa de los tejidos periapicales dañados y contaminados durante el tratamiento quirúrgico es fundamental para el éxito a largo plazo.

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