Análisis de la expresión de RANKL tras la administración de etanol durante la aplicación de fuerza ortodóntica: un estudio in vivo

Autores/as

  • Dwayne Daniel Fredrick Rehatta Resident, Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Autor/a https://orcid.org/0000-0001-7784-3039
  • Ardiansyah S. Pawinru Associate Professor, Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Autor/a https://orcid.org/0000-0002-4763-5439
  • Zulfiani Syachbaniah Lecturer Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Autor/a
  • Baharuddin M. Ranggang Assistant Professor, Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Autor/a https://orcid.org/0009-0002-6573-8922
  • Eddy Heriyanto Habar Associate Professor, Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Autor/a https://orcid.org/0000-0002-4228-0624
  • Eka Erwansyah Associate Professor, Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Autor/a https://orcid.org/0000-0002-1760-2409

DOI:

https://doi.org/10.15517/1db0eq21

Palabras clave:

Fuerza ortodóntica; Etanol; Alcohol; RANKL; Movimiento dentario; Remodelación ósea.

Resumen

La prevalencia del consumo de alcohol sigue siendo considerablemente alta a nivel mundial. A medida que crece la conciencia sobre las maloclusiones, es razonable suponer que algunos pacientes con tratamiento ortodóntico consumen alcohol. Los efectos del consumo de alcohol (etanol) sobre la respuesta al tratamiento ortodóntico aún no están del todo claros.  Analizar las diferencias y los efectos del etanol sobre la expresión de RANKL en el proceso de remodelación ósea durante la aplicación de fuerza ortodóntica. Se realizó un estudio in vivo con 25 ratas Wistar a las que se les colocó un resorte helicoidal cerrado. Los animales se dividieron en cinco grupos: grupo control negativo A (sin fuerza ni etanol) en el día 0; grupo K1 (K=fuerza ortodóntica) en el día 3 y K2 en el día 14; y grupo P1 (P=fuerza ortodóntica+etanol al 20%) en el día 3 y P2 en el día 14. Posteriormente, los animales fueron sacrificados, se realizaron las preparaciones correspondientes y se examinó la expresión de RANKL mediante inmunohistoquímica (IHQ). Los datos se analizaron mediante pruebas t independientes y regresión. La prueba t de muestras independientes mostró diferencias significativas en la expresión de RANKL entre los grupos K y P en los días 3 y 14 (p<0,05). El análisis de regresión indicó que la duración de la administración de etanol afectó significativamente el nivel de expresión de RANKL (p<0,05). Se observaron diferencias en la expresión de RANKL tras la administración de alcohol, así como efectos del etanol sobre la expresión de RANKL en el proceso de remodelación ósea durante la aplicación de fuerza ortodóntica. Se especula que el etanol incrementa la osteoclastogénesis, lo que podría acelerar el movimiento ortodóntico, aunque se requieren más estudios para confirmarlo.

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Publicado

2025-07-14

Número

Vol. 0 Núm. 00 (2025): ONLINE FIRST ODOVTOS-International Journal of Dental Sciences

Sección

Artículos de Investigación Básica

Licencia

Derechos de autor 2025 Dwayne Daniel Fredrick Rehatta, Ardiansyah S. Pawinru, Zulfiani Syachbaniah, Baharuddin M. Ranggang, Eddy Heriyanto Habar, Eka Erwansyah.

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