Keywords
Candida albicans; Enterococcus faecalis; Reciproc; TFA; WaveOne Gold.
How to Cite
Abstract
To evaluate whether the WaveOne Gold and Reciproc single file instrumentation systems, are effective in reducing the microbial load of a mixed biofilm and the cleaning of apical third compared to the Twisted File Adaptive system (multiple-file system). Seventy mesial roots of the first and second molars were included and randomly divided into three experimental groups (n=20, n=10 controls). Biofilms were formed inside canals over 31 days. After instrumentation with the unique file systems, WaveOne Gold and Reciproc and the multiple file system Twisted File Adaptive, using 2.25% sodium hypochlorite as an irrigant in all cases, a count of colony forming units was performed using serial dilutions, cleaning of the apical third was evaluated using scanning electron microscopy. Comparisons amongst groups were made by using parametric and non-parametric statistics, according to a normal or non-normal data distribution, respectively. No significant differences in the reduction of the microbial load after employing a single-file system in comparison to the multiple-file system were found; in addition, the cleaning of the apical third was similar for the three different instrumentation systems. The single-file system is equal in effectiveness compared with the multiple-file system in reducing the microbial load.
References
Mohammadi Z., Jafarzadeh H., Shalavi S., Palazzi F. Recent advances in root canal disinfection: A review. Iran Endod J. 2017; 12 (4): 402-406. doi: 10.22037/iej.v12i4.17935
Olcay K., Ataoglu H., Belli S. Evaluation of related factors in the failure of endodontically treated teeth: A Cross-sectional Study. J Endod. 2018; 44 (1): 38-45. doi: 10.1016/j.joen.2017.08.029
Adams N., Tomson P.L. Access cavity preparation. Br Dent J. 2014; 216 (6): 333-339. doi: 10.1038/sj.bdj.2014.206
Dewhirst F., Chen T., Izard J., et al. The human oral microbiome. J Bacteriol. 2010; 192 (19): 5002-5017. doi: 10.1128/JB.00542-10.
Sakko M., Tjäderhane L., Rautemaa-Richardson R. Microbiology of root canal infections. Prim Dent J. 2016; 5 (2): 84-89. doi: 10.1308/205016816819304231.
Siqueira J.F. Jr., Sen H. Fungi in endodontic infection. Oral Surg Oral Med Oral Pathol. 2004; 97 (5): 632-641. doi: 10.1016/S1079210404000046
Sen B.H., Chugal N.M., Liu H., Fleischmann J. A new method for studying the adhesion of Candida albicans to dentin in the presence or absence of smear layer. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 96 (2): 201-206. doi: 10.1016/s1079-2104(03)00165-3
Siqueira J.F. Jr. Endodontic infections: Concepts, paradigms, and perspectives. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002; 94 (3): 281-293. doi: 10.1067/moe.2002.126163
Stuart H., Schwartz A., Beeson J., Owatz B. Enterococcus faecalis: Its role in root canal treatment failure and current concepts in retreatment. J Endod. 2006; 32 (2): 93-99. doi: 10.1016/j.joen.2005.10.049
Kapil J., Parolia A., Shetty K., Mehta K. Biofilm in endodontic: a Review. J Int Soc Prev Community Dent. 2015; 5 (1): 1-12. doi: 10.4103/2231-0762.151956
Johnson M., Flannagan E., Sedgley E. Coaggregation interactions between oral and endodontic enterococcus faecalis and bacterial species isolated from persistent apical periodontitis. J Endod. 2006; 32 (10): 946-952. doi: 10.1016/j.joen.2006.03.023
Dioguardi M., Troiano G., Laino L., et al. ProTaper and WaveOne systems three-dimensional comparison of device parameters after the shaping technique. A micro-CT study on simulated root canals. Int J Clin Exp Med. 2015; 8 (10): 17830-17834. eCollection 2015.
Marinho A.C., Martinho F.C., Goncalves L.M., Rabang H.R.C., Gomes B.P.F.A . Does the Reciproc file remove root canal bacteria and endotoxins as effectively as multifile rotary systems? Int Endod J. 2015; 48 (6): 542-548. doi: 10.1111/iej.12346
Gonzalez A.M., Corpus E., Pozos-Guillén A., Silva-Herzog D., Aragon-Piña A., Cohenca N. Continuous drip flow system to develop biofilm of E. faecalis under anaerobic conditions. ScientificWorldJournal. 2014; 2014: 1-5. doi: 10.1155/2014/706189
Vaishnavi N. Endodontic microbiology. J Dent Clin. 2010; 13 (4): 15-19. doi: 10.4103/0972-0707.73386
Yared G. Canal preparation using only one Ni-Ti rotary instrument: preliminary observations. Int Endod J. 2008; 41 (4): 339-344. doi: 10.1111/j.1365-2591.2007.01351.x
Gambarini G., Piasecki L., Di Nardo D., et al. Incidence of deformation and fracture of twisted file adaptive instruments after repeated clinical use. J Oral Maxillofac Res. 2016; 7 (4): e5. doi: 10.5037/jomr.2016.7405
Tennert C., Fuhrmann M., Wittmer A., et al. New bacterial composition in primary and persistent/Secondary endodontic infection with respect to clinical and radiographic findings. J Endod. 2014; 40 (5): 670-677. doi: 10.1016/j.joen.2013.10.005
Nair P.N. Endodontic biofilm, technology and pulpal regenerative therapy: where do we go from here? Int Endod J. 2014; 47 (11): 1003-1011. doi: 10.1111/iej.12287
Chandra J., Kuhn D., Mukherjee P., Hoyer L.L., McCormick T., Ghannoum M.A. Biofilm formation by the fungal pathogen Candida albicans: Development, architecture, and drug resistance. J Bacteriol. 2001; 183 (18): 5385-5394. doi: 10.1128/JB.183.18.5385-5394.2001
Yang Y., Shen Y., Wang Z., et al. Evaluation of the susceptibility of multispecies biofilms in dentinal tubules to disinfecting solutions. J Endod. 2016; 42 (8): 1246-1250. doi: 10.1016/j.joen.2016.05.011
Van der Waal S., de Almeida J., Krom B.P., de Soet J.J., Crielaard W. Diffusion of antimicrobials in multispecies biofilms evaluated in a new biofilm model. Int Endod J. 2017; 50 (4): 367-376. doi: 10.1111/iej.12634
Ghivari S.B., Bhattacharya H., Bhat K.G., Pujar M.A. Antimicrobial activity of root canal irrigants against biofilm forming pathogens- An in vitro study. J Conserv Dent. 2017; 20 (3): 147-151. doi: 10.4103/JCD.JCD_38_16
Dhingra A., Ruhal N., Miglani A. Evaluation of single file systems Reciproc, Oneshape, and WaveOne using cone beam computed tomography -an in vitro study. J Clin Diagn Res. 2015; 9 (4): ZC30-ZC34. doi: 10.7860/JCDR/2015/12112.5803
Bürklein S., Hinschitza K., Dammaschke T., Schäfer E. Shaping ability and cleaning effectiveness of two single-file systems in severely curved root canals of extracted teeth: Reciproc and WaveOne versus Mtwo and ProTaper. Int Endod J. 2012; 45 (5): 449-461. doi: 10.1111/j.1365-2591.2011.01996.x
De Oliveira B.P., Aguiar C.M., Câmara A.C., et al. Evaluation of microbial reduction in root canals instrumented with reciprocating and rotary systems. Acta Stomatol Croat. 2015; 49 (4): 294-303. doi: 10.15644/asc49/4/4
Carvalho M. de S., Junior E.C., Bitencourt Garrido A.D., Roberti Garcia L.F., Franco Marques A.A. Histological evaluation of the cleaning effectiveness of two reciprocating single-file systems in severely curved root canals: Reciproc versus WaveOne. Eur J Dent. 2015; 9 (1): 80-86. doi: 10.4103/1305-7456.149648
Ahlquist M., Henningsson O., Hultenby K. The effectiveness of manual and rotary techniques in the cleaning of root canals: a scanning electron microscopy study. Int Endod J. 2001; 34 (7): 533-537. doi: 10.1046/j.1365-2591.2001.00429.x
Bürklein S., Flüch S., Schäfer E. Shaping ability of reciprocating single-file systems in severely curved canals: WaveOne and Reciproc versus WaveOne Gold and Reciproc blue. Odontology. 2018; 107 (1): 96-102. doi: 10.1007/s10266-018-0364-3
Üreyen Kaya B., Erik C.E., Sesli Çetin E., Köle M., Maden M. Mechanical reduction in intracanal Enterococcus faecalis when using three different single-file systems: an ex vivo comparative study. Int Endod J. 2018; 52 (1): 77-85. doi: 10.1111/iej.12984
Siqueira J.F. Jr., Rôças I.N., Favieri A., Lima K.C. Chemomechanical reduction of the bacterial population in the root canal after intrumentation and irrigation with 1%, 2.5% and 5.25% sodium hypoclorite. J Endod. 2010; 26 (6): 331-334. doi: 10.1097/00004770-200006000-00006
Rôças I.N., Lima K., Siqueira J.F. Jr. Reduction in bacterial counts in infected root canals after rotary or hand nickel titanium instrumentation-a clinical study. Int Endod J. 2013; 46 (7): 681-687. doi: 10.1111/iej.12045.
Topçuoğlu H.S., Düzgün S., Aktı A., Topçuoğlu G. Laboratory comparison of cyclic fatigue resistance of WaveOne Gold, Reciproc and WaveOne files in canals with a double curvature. Int Endod J. 2017; 50 (7): 713-717. doi: 10.1111/iej.12674
Comments
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Copyright (c) 2022 CC-BY-NC-SA 4.0