Keywords
Endodoncia; Dentina; Instrumentos de níquel y titanio; Preparación de Conductos radiculares; Cavidad de la pulpa dental; Microtomografía de rayos X.
How to Cite
Abstract
The aim of this study was to assess the influence of micro-computed tomography (micro-CT) voxel size on evaluation of root canal preparation using rotary heat-treated nickel-titanium files. Curved mesial root canals of mandibular molars were prepared using ProDesign Logic 30/.05 (PDL) or HyFlex EDM 25/.08 (HEDM) (n=12). The specimens were scanned using micro-CT with 5μm of voxel size before and after root canal preparation. Images with sub-resolution of 10 and 20μm voxel sizes were obtained. The percentage of volume increase, debris and uninstrumented root canal surface were analyzed in the different voxel sizes. Data were compared using unpaired Student’s t-test and ANOVA statistical tests (α=0.05). No differences were observed for percentage of volume increase, debris and instrumented surface between the root canals prepared by PDL and HEDM (p>0.05). Both systems promoted higher percentage of debris in the apical third compared to the middle third (p<0.05). After instrumentation using PDL the percentage of uninstrumented surface was highr in the apical third than middle third only when analysis were performed at 5µm (p<0.05). When comparing the different voxel sizes (5,10 or 20µm), both groups showed different means for the variables, with no significant difference (p>0.05). PDL and HEDM had similar root canal preparation capacity. Micro-CT images using different voxel sizes did not influence the results of volume increase and debris evaluation. However, images at 5µm showed greater accuracy to evaluate the percentage of uninstrumented surfaces.
References
Wu M.K., Dummer P.M., Wesselink P.R. Consequences of and strategies to deal with residual post-treatment root canal infection. Int Endod J. 2006 May; 39 (5): 343-56.
Wu M.K., Dummer PM, Wesselink PR. Consequences of and strategies to deal with residual post-treatment root canal infection. Int Endod J. 2006 May;39 (5): 343-56.
Azim A.A., Griggs J.A., Huang G.T. The Tennessee study: factors affecting treatment outcome and healing time following nonsurgical root canal treatment. Int Endod J. 2016 Jan; 49 (1): 6-16.
Alcalde M.P., Duarte M.A.H., Bramante C.M., de Vasconselos B.C., Tanomaru-Filho M., Guerreiro-Tanomaru J.M., Pinto J.C., Só M.V.R., Vivan R.R. Cyclic fatigue and torsional strength of three different thermally treated reciprocating nickel-titanium instruments. Clin Oral Investig. 2018 May; 22 (4): 1865-1871.
Pinheiro S.R, Alcalde M.P., Vivacqua-Gomes N., Bramante C.M., Vivan R.R., Duarte M.A.H., Vasconcelos B.C. Evaluation of apical transportation and centring ability of five thermally treated NiTi rotary systems. Int Endod J. 2018 Jun; 51 (6): 705-713.
Pinto J.C., Pivoto-João M.M.B., Espir C.G., Ramos M.L.G., Guerreiro-Tanomaru J.M., Tanomaru-Filho M. Micro-CT evaluation of apical enlargement of molar root canals using rotary or reciprocating heat-treated NiTi instruments. J Appl Oral Sci. 2019 Aug 12; 27: e20180689.
Rodrigues C.T., Duarte M.A., de Almeida M.M., de Andrade F.B., Bernardineli N. Efficacy of CM-Wire, M-Wire, and Nickel-Titanium Instruments for Removing Filling Material from Curved Root Canals: A Micro-Computed Tomography Study. J Endod. 2016 Nov; 42 (11): 1651-1655.
Pedullà E., Genovesi F., Rapisarda S., La Rosa G.R., Grande N.M., Plotino G., Adorno C.G. Effects of 6 Single-File Systems on Dentinal Crack Formation. J Endod. 2017 Mar; 43 (3): 456-461.
Iacono F., Pirani C., Generali L., Bolelli G., Sassatelli P., Lusvarghi L., Gandolfi M.G., Giorgini L., Prati C. Structural analysis of HyFlex EDM instruments. Int Endod J. 2017 Mar; 50 (3): 303-313.
Pirani C., Iacono F., Generali L., Sassatelli P., Nucci C., Lusvarghi L., Gandolfi M.G., Prati C. HyFlex EDM: superficial features, metallurgical analysis and fatigue resistance of innovative electro discharge machined NiTi rotary instruments. Int Endod J. 2016 May; 49 (5): 483-93.
da Silva Limoeiro A.G., Dos Santos A.H., De Martin A.S., Kato A.S., Fontana C.E., Gavini G., Freire L.G., da Silveira Bueno C.E. Micro-Computed Tomographic Evaluation of 2 Nickel-Titanium Instrument Systems in Shaping Root Canals. J Endod. 2016 Mar; 42 (3): 496-9.
Stringheta C.P., Bueno C.E.S., Kato A.S., Freire L.G., Iglecias E.F., Santos M., Pelegrine R.A. Micro-computed tomographic evaluation of the shaping ability of four instrumentation systems in curved root canals. Int Endod J. 2019 Jun; 52 (6): 908-916.
Bouxsein M.L., Boyd S.K., Christiansen B.A., Guldberg R.E., Jepsen K.J., Müller R. Guidelines for assessment of bone microstructure in rodents using micro-computed tomography. J Bone Miner Res. 2010 Jul; 25 (7): 1468-86.
Leoni G.B., Versiani M.A., Silva-Sousa Y.T., Bruniera J.F., Pécora J.D., Sousa-Neto M.D. Ex vivo evaluation of four final irrigation protocols on the removal of hard-tissue debris from the mesial root canal system of mandibular first molars. Int Endod J. 2017 Apr; 50 (4): 398-406.
Pivoto-João M.M.B., Tanomaru-Filho M., Pinto J.C., Espir C.G., Guerreiro-Tanomaru J.M. Root Canal Preparation and Enlargement Using Thermally Treated Nickel-Titanium Rotary Systems in Curved Canals. J Endod. 2020 Nov; 46 (11): 1758-1765.
Zuolo M.L., Zaia A.A., Belladonna F.G., Silva E.J.N.L., Souza E.M., Versiani M.A., Lopes R.T., De-Deus G. Micro-CT assessment of the shaping ability of four root canal instrumentation systems in oval-shaped canals. Int Endod J. 2018 May; 51 (5): 564-571.
Zhao Y., Fan W., Xu T., Tay F.R., Gutmann J.L., Fan B. Evaluation of several instrumentation techniques and irrigation methods on the percentage of untouched canal wall and accumulated dentine debris in C-shaped canals. Int Endod J. 2019 Sep; 52 (9):1354-1365.
Siqueira J.F. Jr., Alves F.R., Versiani M.A., Rôças I.N., Almeida B.M., Neves M.A., Sousa-Neto M.D. Correlative bacteriologic and micro-computed tomographic analysis of mandibular molar mesial canals prepared by self-adjusting file, reciproc, and twisted file systems. J Endod. 2013 Aug; 39 (8): 1044-50.
Zhao D., Shen Y., Peng B., Haapasalo M. Micro-computed tomography evaluation of the preparation of mesiobuccal root canals in maxillary first molars with Hyflex CM, Twisted Files, and K3 instruments. J Endod. 2013 Mar; 39 (3): 385-8.
Peters O.A., Arias A., Paqué F. A Micro-computed Tomographic Assessment of Root Canal Preparation with a Novel Instrument, TRUShape, in Mesial Roots of Mandibular Molars. J Endod. 2015 Sep; 41 (9): 1545-50.
Vertucci F.J. Root canal anatomy of the human permanent teeth. Oral Surg Oral Med Oral Pathol. 1984 Nov; 58 (5): 589-99.
Schneider S.W. A comparison of canal preparations in straight and curved root canals. Oral Surg Oral Med Oral Pathol. 1971 Aug; 32 (2): 271-5.
Pruett J.P., Clement D.J., Carnes D.L. Jr. Cyclic fatigue testing of nickel-titanium endodontic instruments. J Endod. 1997 Feb; 23 (2): 77-85.
Isaksson H., Töyräs J., Hakulinen M., Aula A.S., Tamminen I., Julkunen P., Kröger H., Jurvelin J.S. Structural parameters of normal and osteoporotic human trabecular bone are affected differently by microCT image resolution. Osteoporos Int. 2011 Jan; 22 (1): 167-77.
Pinto J.C., Torres F.F.E., Santos Junior A.O., Tavares K.I.M.C., Guerreiro-Tanomaru J.M., Tanomaru-Filho M. Influence of voxel size on micro-CT analysis of debris after root canal preparation. Braz Oral Res. 2020 Nov 13; 35: e008.
Pinto J.C., Coaguila-Llerena H., Torres F.F.E., Lucas-Oliveira É., Bonagamba T.J., Guerreiro-Tanomaru J.M., Tanomaru-Filho M. Influence of voxel size on dentinal microcrack detection by micro-CT after root canal preparation. Braz Oral Res. 2021 Oct 11; 35: e074.
Freire L.G., Iglecias E.F., Cunha R.S., Dos Santos M., Gavini G. Micro-Computed Tomographic Evaluation of Hard Tissue Debris Removal after Different Irrigation Methods and Its Influence on the Filling of Curved Canals. J Endod. 2015 Oct; 41 (10): 1660-6.
Sode M., Burghardt A.J., Nissenson R.A., Majumdar S. Resolution dependence of the non-metric trabecular structure indices. Bone. 2008 Apr; 42 (4): 728-36.
Christiansen B.A. Effect of micro-computed tomography voxel size and segmentation method on trabecular bone microstructure measures in mice. Bone Rep. 2016 May 27; 5: 136-40.
Orhan K., Jacobs R., Celikten B., Huang Y., de Faria Vasconcelos K., Nicolielo L.F.P., Buyuksungur A., Van Dessel J. Evaluation of Threshold Values for Root Canal Filling Voids in Micro-CT and Nano-CT Images. Scanning. 2018 Jul 16; 2018: 9437569.
Paqué F., Laib A., Gautschi H., Zehnder M. Hard-tissue debris accumulation analysis by high-resolution computed tomography scans. J Endod. 2009 Jul; 35 (7): 1044-7.
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