Phosphate-mineralizing Actinomycetes involved in root interaction of Glomus sp.- white clover
Keywords:Streptomyces sp., phosphatases, solubilization, arbuscular mycorhiza.
The objective of this work was to determine the capacity of actinomicetes for organic phosphorus mineralization and their rhyzospere
interaction with mycorrhiza of Trifolium repens L. (white
clover). In January 2009, at the laboratory of the Unidad de
Investigaciones Agropecuarias (UNIDIA) of the Pontifi cia
Universidad Javeriana, Bogotá, Colombia, the phosphatase
activity of Streptomyces sp. (strain MCR9), Streptomyces
sp. (strain MCR26) and Thermobifi da sp. (strain MCR24)
were determined using the p-nitrofenil phosphate technique.
Thermobifi da sp. (strain MCR24) showed the lowest activity.
Inoculation of actinomycetes alone resulted in higher values
of inorganic soil P, while leaf P content was higher when coinoculation
of actinomycete-Glomus sp. occurred, with the
exception of the strain Thermobifi da sp. (strain MCR24). Coinoculation with actynomicetes and Glomus sp. stimulated
biomass production and colonization percent. Actynomicetes
are sinergistic with Glomus sp., resulting in benefi ts for the
plant and the mycorrhiza. Analysis of variance and “t” tests
ALS (P<0,05) were conducted to compare the treatments.
The relation between the degree of mycorrhization plant dry
weight was determined by the “r” correlation coeffi cient, and
plants associated with microorganisms was higher than 70%.
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