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Copyright
© Agronomía Mesoamericana, 2015
Affiliations
Luis Alvarado-Marchena
Instituto Tecnológico de Costa Rica (ITCR), Escuela de Biología. Cartago, Costa Rica.
Dora Flores-Mora
Instituto Tecnológico de Costa Rica (ITCR), Escuela de Biología. Cartago, Costa Rica.
Randall Chacón-Cerdas
Instituto Tecnológico de Costa Rica (ITCR), Escuela de Biología. Cartago, Costa Rica.
Alexander Schmidt-Durán
Instituto Tecnológico de Costa Rica (ITCR), Escuela de Biología. Cartago, Costa Rica.
Carlos Alvarado-Ulloa
Instituto Tecnológico de Costa Rica (ITCR), Escuela de Biología. Cartago, Costa Rica.
How to Cite
Comments
Molecular characterization of two accessions of Cydonia oblonga.
- Luis Alvarado-Marchena ,
- Dora Flores-Mora ,
- Randall Chacón-Cerdas ,
- Alexander Schmidt-Durán ,
- Carlos Alvarado-Ulloa
Agronomía Mesoamericana: Vol. 26, Issue 2 (July-December)
Published: Jun 16, 2015
Abstract
The purpose of this research was to evaluate the plastid trnH-psbA spacer and the matK coding sequence as possible markers for the molecular characterization in two accessions of quince (C. oblonga), one from Argentina and the other one from Costa Rica. The study was carried out at Center for Biotechnology Research (CIB in Spanish) of the Technological Institute of Costa Rica (ITCR, in Spanish) during 2014. Amplified products were analyzed using MEGA v6.0 software to calculate genetic distances using the Tamura-Nei model.The results showed that the matK sequence presented the highest percentage of variable sites and the highest intraspecific distance, suggesting the presence of polymorphisms between the two accessions. On the other hand, the trnH-psbA spacer for quince had a low ability to discriminate because, at intraspecific level, this locus showed less differences.