Trichoderma growth in pineapple stubble to obtain spores for agricultural use
DOI:
https://doi.org/10.15517/am.v31i3.40275Keywords:
conidia, pineapple waste, growth kinetics, agroindustryAbstract
Introduction. The use of biological controllers as alternatives to traditional chemicals is an ecological and economic option for farmers. Fungi of the genus Trichoderma can confer resistance to plants and increase the absorption of nutrients, reducing a wide variety of crop diseases. Objective. To evaluate pineapple stubble wastes as a substrate for the solid fermentation growth of two Trichoderma strains to produce a spore solution for agricultural purposes. Materials and methods. The experiment was carried out at the Laboratory of Research in Chemistry and Applied Biosciences of the Universidad Tecnica Nacional of Costa Rica, in the period between April 2018 and October 2019. Two variants of fungus T. viride and T. harzianum were used, these were grown in natural potato dextrose agar (PDA) and pineapple stubble. A conidia count was performed in a 1/10 mm Neubauer chamber. Results. T. viride and T. harzianum showed uniform growth in reseeding plates with color changes in time, from white to green. T. viride showed better development in wet substrate, while T. harzianum in low moisture stubble. The maximum cell concentration in the liquid solution of T. harzianum and T. viride was 1 132 750 cell ml-1 and 1 023 250 cell ml-1, respectively. Conclusion. Both strains of Trichoderma developed adequately in the pineapple stubble, however, the efficient growth of the strains is associated with the management of external factors such as the amount of substrate, oxygen, and moisture.
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