Detection of IHHNV in Litopenaeus vannamei farms in Costa Rica

Authors

DOI:

https://doi.org/10.15517/am.v32i2.43179

Keywords:

infectious disease, genotyping, pathogenic lineages

Abstract

Introduction. The infectious hypodermal and haematopoietic necrosis virus (IHHNV) produces cuticular deformities and growth reduction of white shrimp (Litopenaeus vannamei) causing losses due to a lower harvest weight and lower market prices. Although different pathogenic and non-pathogenic genotypes of IHHNV have been described, it is unknown whether these are present in the country. Objective. To characterize the IHHNV lineages present in white shrimp farms in Costa Rica. Materials and methods. Between 2017 and 2018, a total of 15 shrimp farms distributed in the Gulf of Nicoya, Guanacaste, Costa Rica were investigated. Water, postlarvae, and juvenile shrimp samples were collected during a production cycle of three months, additionally, physicochemical parameters of the water in the ponds were measured and a survey was applied to the producers, to know if they had knowledge about the disease and if they suspected the presence of the agent in their establishments. The collected samples were analyzed by Polymerase Chain Reaction (PCR) and sequencing. Results. The presence of IHHNV was determined in 86.6 % (13/15) of the farms analyzed, the sequencing of the amplified products determined the presence of IHHNV lineage III, with 99.2 %-100.0 % (386-389/389 bp) similarity with the sequence isolated from L. vannamei in Venezuela (KM485615.1). Poor physicochemical parameters of the water were determined. A total of 53.3 % of producers had not heard of IHHNV, while the remaining producers suspected having the virus on their farms. Conclusion. IHHNV pathogenic lineage III was present in most of the farms. It is recommended to train producers and establish welfare, management, and biosecurity protocols in order to improve the productive parameters of their farms.

Downloads

Download data is not yet available.

References

Cowley, J. A., Rao, M., & Coman, G. J. (2018). Real-time PCR tests to specifically detect IHHNV lineages and an IHHNV EVE integrated in the genome of Penaeus monodon. Diseases of aquatic organisms, 129(2), 145-158. https://doi.org/10.3354/dao03243

Cuéllar-Anjel, J. (2013). Necrosis infecciosa hipodérmica y hematopoyética (IHHNV). Center for Food Security and Public Health. http://www.cfsph.iastate.edu/Factsheets/es/infectious_hypodermal_and_hematopoietic_necrosis-es.pdf

Dhar, A. K., Roux, M. M., & Klimpel, K. R. (2001). Detection and quantification of infectious hypodermal and hematopoietic necrosis virus and white spot virus in shrimp using real-time quantitative PCR and SYBR Green chemistry. Journal of Clinical Microbiology, 39(8), 2835–2845. https://doi.org/10.1128/JCM.39.8.2835-2845.2001

Food & Drug Administration. (2018). Hazard analysis critical control point (HACCP). Retrieved September, 2018, from https://www.fda.gov/food/guidance-regulation-food-and-dietary-supplements/hazard-analysis-critical-control-point-haccp

Felsenstein, J. (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution; International Journal of Organic Evolution, 39(4), 783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x

Glover, K. L., Nunan, L. M., & Lightner, D. V. (1995). Measurement using polymerase chain reaction (PCR) of the survival of infectious hypodermal and hematopoietic necrosis virus (IHHNV) subjected to shrimp culture disinfection techniques. In C. Browdy, & S. Hopkins (Eds.), Swimming Through Troubled Water. Proceedings of the Special Session on Shrimp Farming (p. 239). World Aquaculture Society.

Hall, T. A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT.Nucleic Acids Symposium Series, 41, 95-98. https://doi.org/10.14601/Phytopathol_Mediterr-14998u1.29

Kalagayan, H., Godin, D., Kanna, R., Hagino, G., Sweeney, J., Wyban, J., & Brock, J. (1991). IHHN virus as an etiological factor in runt-deformity syndrome (RDS) of juvenile Penaeus vannamei cultured in Hawaii. Journal of the World Aquaculture Society, 22(4), 235-243. https://doi.org/10.1111/j.1749-7345.1991.tb00740.x

Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547-1549. https://doi.org/10.1093/molbev/msy096

Lightner, D. V. (2003). Exclusion of specific pathogens for disease prevention in a penaeid shrimp biosecurity program. In C. Lee, & P. O’Bryen (Eds.), Biosecurity in aquaculture production systems. Exclusion of pathogens and other undesirables (pp. 81-116). World Aquaculture Society.

Montgomery-Brock, B. D., Tacon, A. G., Poulos, B., & Lightner, D. V. (2007). Reduced replication of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in Litopenaeus vannamei held in warm water. Aquaculture, 265(1-4), 41-48. https://doi.org/10.1016/j.aquaculture.2007.01.025

Morales-Covarrubias, M., Ruiz-Luna, A., Moura-Lemus, A.P., Solís, V. T., & Conroy, G. (2011). Prevalencia de enfermedades de camarón blanco (Litopenaeus vannamei) cultivado en ocho regiones de Latinoamérica. Revista científica FCVLUZ, 21(5), 434-446. https://www.redalyc.org/articulo.oa?id=95919362010

Nunan, L. M., Poulos, B. T., & Lightner, D. V. (2000). Use of polymerase chain reaction for the detection of infectious hypodermal and hematopoietic necrosis virus in penaeid shrimp. Marine Biotechnology, 2, 319-328. https://doi.org/10.1007/s101260000003

Organización Mundial de Sanidad Animal. (2015). Manual de las pruebas de diagnóstico para los animales acuáticos. Recuperado en septiembre de 2017. http://www.oie.int/es/normas-internacionales/manual-acuatico/acceso-en-linea/

Organización Mundial de Sanidad Animal. (2017). Infection with infectious hypodermal and haematopoietic necrosis virus. Recuperado en setiembre 2017 de http://www.oie.int/es/normas-internacionales/manual-acuatico/acceso-en-linea/

Organización Mundial de Sanidad Anima. (2019). Infección por el virus de la necrosis hipodérmica y hematopoyética infecciosa. Recuperado en enero de 2019 de https://www.oie.int/index.php?id=2439&L=2&htmfile=chapitre_ihhn.htm

Peña-Navarro, N., & Varela-Mejías, A. (2016). Prevalencia de las principales enfermedades infecciosas en el camarón blanco Penaeus vannamei cultivado en el Golfo de Nicoya, Costa Rica. Revista de Biología Marina y Oceanografía, 51(3), 553-564. https://dx.doi.org/10.4067/S0718-19572016000300007

Puente, E. (2009). Respuestas fisiológicas de juveniles de camarón blanco Litopenaeus vannamei, a condiciones oscilantes de oxígeno disuelto y temperatura [Tesis de Doctorado, Instituto Politécnico Nacional]. Repositorio del Instituto Politécnico Nacional. http://www.remeri.org.mx/portal/REMERI.jspid=oai:www.repositoriodigital.ipn.mx:123456789/14322

Robles-Sikisaka, R., Bohonak, A. J., McClenaghan, L. R., Jr, & Dhar, A. K. (2010). Genetic signature of rapid IHHNV (infectious hypodermal and hematopoietic necrosis virus) expansion in wild Penaeus shrimp populations. PloS One, 5(7), Article e11799. https://doi.org/10.1371/journal.pone.0011799

Silva, D. C., Nunes, A. R., Teixeira, D. I., Lima, J. P., & Lanza, D. C. (2014). Infectious hypodermal and hematopoietic necrosis virus from Brazil: Sequencing, comparative analysis and PCR detection. Virus Research, 189, 136-146. https://doi.org/10.1016/j.virusres.2014.05.008

Shike, H., Dhar, A. K., Burns, J. C., Shimizu, C., Jousset, F. X., Klimpel, K. R., & Bergoin, M. (2000). Infectious hypodermal and hematopoietic necrosis virus of shrimp is related to mosquito brevidensoviruses. Virology, 277(1), 167–177. https://doi.org/10.1006/viro.2000.0589

Tang, K. F., & Lightner, D. V. (2002). Low sequence variation among isolates of infectious hypodermal and hematopoietic necrosis virus (IHHNV) originating from Hawaii and the Americas. Diseases of Aquatic Organisms, 49(2), 93–97. https://doi.org/10.3354/dao049093

Tang, K. F., Navarro, S. A., & Lightner, D. V. (2007). PCR assay for discriminating between infectious hypodermal and hematopoietic necrosis virus (IHHNV) and virus-related sequences in the genome of Penaeus monodon. Diseases of Aquatic Organisms, 74(2), 165–170. https://doi.org/10.3354/dao074165

Vanpatten, K. A., Nunan, L. M., & Lightner, D. V. (2004). Seabirds as potential vectors of penaeid shrimp viruses and the development of a surrogate laboratory model utilizing domestic chickens. Aquaculture, 241(1-4), 31-46. https://doi.org/10.1016/j.aquaculture.2004.08.012

Published

2021-05-01

How to Cite

Parajeles Mora, J. F., Peña Navarro, N., Solorzano Morales, A., & Dolz, G. (2021). Detection of IHHNV in Litopenaeus vannamei farms in Costa Rica. Agronomía Mesoamericana, 32(2), 587–598. https://doi.org/10.15517/am.v32i2.43179