In order to study the effectvof biofertilization of Coffea arabica L. with Azospirillumvbrasilense and Glomus intraradice a trial was established ina nursery at the experimental station Rosario Izapa, Chiapas, Mexico from September 2005 to April 2006. We used a Andosol-mollic soil typical of the Soconusco coffee region of Chiapas, Mexico. A. brasilense had a concentration of 100x 106 bacteria per gram of peat and the mycorrhizal fungi of 40 spores per gram of soil with 95% infection of the root system of onion. The treatments were the microorganism salone, the combination of both, and the control without microorganism, which were arranged in a randomized complete block design. Morphological and physiological yield components were evaluated every 30 days from the second month to seven months. The content of N, P and Ca in planttissue was determined. The results were analyzed statistically and differences between treatments were compared according to Tukey 5%. The results indicate a differential response of microorganisms. A. brasilense induced greaterroot development and the double symbiosis G. intraradices+ A. brasilense enhanced the development of stems and leaf blades. The content of N, P and Ca are presented in a manner consistent with G. intraradices.
Keywords: Inoculation, allocation of dry matter, nitrogen, phosphorous, calcium, coffee.