Keywords
Periodontitis; Células Th17; Células Treg; Artritis reumatoide.
How to Cite
Abstract
Th17/Treg cells and P. gingivalis have been implicated in rheumatoid arthritis (RA) and periodontitis (PE). The aim was to determine and compare Th17/Treg cells and the influence of P. gingivalis on this cell population in healthy subjects (HS) and patients with PE, RA, and PE/RA. The number of copies of P. gingivalis was performed by real-time PCR and flow cytometry was used to analyze Th17/Treg cells. In this study, PE in patients with RA increased the number of DNA copies of P. gingivalis, increasing the percentages of Th17 cells, as well as the number and regulation of Treg cells. Periodontitis influences the immune system, increasing the percentage of pro-inflammatory cells in patients with rheumatoid arthritis, which may could influence the inflammatory condition of this population.
References
McInnes, I. B., & Schett, G. Pathogenetic insights from the treatment of rheumatoid arthritis. Lancet (London, England). 2000; 389 (10086): 2328-2337.
Noack, M., & Miossec, PTh17 and regulatory T cell balance in autoimmune and inflammatory diseases. Autoimmunity reviews. 2014; 13 (6): 668-677.
Wang, T., Sun, X., Zhao, J., et al. Regulatory T cells in rheumatoid arthritis showed increased plasticity toward Th17 but retained suppressive function in peripheral blood. Annals of the rheumatic diseases. 2015; 74 (6): 1293-1301.
Paradowska-Gorycka, A., Wajda, A., Romanowska-Próchnicka, K., et al. Th17/Treg-Related Transcriptional Factor Expression and Cytokine Profile in Patients with Rheumatoid Arthritis. Frontiers in immunology. 2020;11: 572858.
Boniface, K., Blom, B., Liu, Y. J., & de Waal Malefyt, R. From interleukin-23 to T-helper 17 cells: human T-helper cell differentiation revisited. Immunological reviews. 2008; 226, 132-146.
Graves, D. T., & Cochran, D. The contribution of interleukin-1 and tumor necrosis factor to periodontal tissue destruction. Journal of periodontology. 2003; 74 (3): 391-401.
Vernal, R., Dutzan, N., Chaparro, A., Puente, J., Antonieta Valenzuela, M., & Gamonal, J. Levels of interleukin-17 in gingival crevicular fluid and in supernatants of cellular cultures of gingival tissue from patients with chronic periodontitis. Journal of clinical periodontology. 2005; 32 (4): 383-389.
Cardoso, C. R., Garlet, G. P., Crippa, G. E., et al. Evidence of the presence of T helper type 17 cells in chronic lesions of human periodontal disease. Oral microbiology and immunology. 2009; 24 (1): 1-6.
Boyle, W. J., Simonet, W. S., & Lacey, D. L. Osteoclast differentiation and activation. Nature. 2003; 423 (6937): 337-342.
Mikuls, T. R., Payne, J. B., Yu, F., et al. Periodontitis and Porphyromonas gingivalis in patients with rheumatoid arthritis. Arthritis & rheumatology (Hoboken, N.J.). 2014; 66 (5): 1090-1100.
Aletaha D., Neogi T., Silman A.J., et al. The 2010 Rheumatoid Arthritis/Classification Criteria: an American College of Rheumatology/European League Against Rheumatism Collaborative Initiative. Arthritis Rheum. 2010; 62: 2569-2581.
Wiebe, C. B., & Putnins, E. E. The periodontal disease classification system of the American Academy of Periodontology--an update. Journal (Canadian Dental Association). 2000; 66 (11): 594-597.
Martinez-Martinez R.E., Abud-Mendoza C., Patiño-Marin N., et al. Detection of periodontal bacterial DNA in serum and synovial fluid in refractory rheumatoid arthritis patients. J Clin Periodontol. 2009; 36 (12): 1004-1010.
Amano A., Nakagawa I., Kataoka K., et al. Distribution of Porphyromonas gingivalis strains with fimA genotypes in periodontitis patients. J Clin Microbiol. 1999; 37 (5): 1426-1430.
Boutaga K., van Winkelhoff A.J., Vandenbroucke-Grauls C.M., et al. Comparison of real-time PCR and culture for detection of Porphyromonas gingivalis in subgingival plaque samples. J Clin Microbiol. 2003; 41 (11): 4950-4954.
Nakagawa I., Amano A., Ohara-Nemoto Y., et al. Identification of a new variant of fimA gene of Porphyromonas gingivalis and its distribution in adults and disabled populations with periodontitis. J Periodontal Res. 2002; 37 (6): 425-432.
Amano A., Kuboniwa M., Nakagawa I., et al. Prevalence of specific genotypes of Porphyromonas gingivalis fimA and periodontal health status. J Dent Res. 2000; 79 (9):1664-1668.
Kobayashi T., Yoshie H. Host Responses in the Link Between Periodontitis and Rheumatoid Arthritis. Curr Oral Health Rep. 2015; 2 (1): 1-8.
Rodríguez-Lozano, B., González-Febles, J., Garnier-Rodríguez, J. L., et al. Association between severity of periodontitis and clinical activity in rheumatoid arthritis patients: a case-control study. Arthritis research & therapy. 2019; 21 (1): 27.
de Pablo, P., Chapple, I. L., Buckley, C. D, et al. Periodontitis in systemic rheumatic diseases. Nature reviews. Rheumatology. 2009; 5 (4): 218-224.
de Pablo, P., Dietrich, T., & McAlindon, T. E. Association of periodontal disease and tooth loss with rheumatoid arthritis in the US population. The Journal of rheumatology. 2008; 35 (1): 70-76.
Furuya, T., Inoue, E., Tanaka, E., et al. Age and female gender associated with periodontal disease in Japanese patients with rheumatoid arthritis: Results from self-reported questionnaires from the IORRA cohort study. Modern rheumatology. 2020; 30 (3): 465-470.
Joseph, R., Rajappan, S., Nath, S. G., & Paul, B. J. Association between chronic periodontitis and rheumatoid arthritis: a hospital-based case-control study. Rheumatology international. 2013; 33 (1): 103-109.
Bıyıkoğlu, B., Buduneli, N., Aksu, K., et al. Periodontal therapy in chronic periodontitis lowers gingival crevicular fluid interleukin-1beta and DAS28 in rheumatoid arthritis patients. Rheumatology international. 2013; 33 (10): 2607-2616.
Lee, J. Y., Choi, I. A., Kim, J. H., et al. Association between anti-Porphyromonas gingivalis or anti-α-enolase antibody and severity of periodontitis or rheumatoid arthritis (RA) disease activity in RA. BMC musculoskeletal disorders. 2015;16: 190.
McGraw, W. T., Potempa, J., Farley, D., et al. Purification, characterization, and sequence analysis of a potential virulence factor from Porphyromonas gingivalis, peptidylarginine deiminase. Infection and immunity.1999; 67 (7): 3248-3256.
Schellekens, G. A., de Jong, B. A., van den Hoogen, F. H., et al. Citrulline is an essential constituent of antigenic determinants recognized by rheumatoid arthritis-specific autoantibodies. The Journal of clinical investigation. 1998; 101 (1): 273-281.
Tesmer, L. A., Lundy, S. K., Sarkar, S., et al. Th17 cells in human disease. Immunological reviews. 2008; 223: 87-113.
Zhao, L., Zhou, Y., Xu, Y., et al. Effect of non-surgical periodontal therapy on the levels of Th17/Th1/Th2 cytokines and their transcription factors in Chinese chronic periodontitis patients. Journal of clinical periodontology. 2011; 38 (6): 509-516.
Wang, L., Wang, J., Jin, Y., et al. Oral administration of all-trans retinoic acid suppresses experimental periodontitis by modulating the Th17/Treg imbalance. Journal of periodontology. 2014; 85 (5): 740-750.
Mellado, M., Martínez-Muñoz, L., Cascio, G., et al. T Cell Migration in Rheumatoid Arthritis. Frontiers in immunology. 2015; 6: 384.
Jin, Y., Wang, L., Liu, D., et al. Tamibarotene modulates the local immune response in experimental periodontitis. International immunopharmacology. 2014; 23 (2): 537-545.
Jiang, Q., Yang, G., Liu, Q., et al. Function and Role of Regulatory T Cells in Rheumatoid Arthritis. Frontiers in immunology. 2021; 12: 626193.
Comments
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Copyright (c) 2024 CC-BY-NC-SA 4.0