Abstract
Although professional prophylaxis is beneficial in controlling dental biofilm, its effects on tooth surfaces with white spot lesions are still poorly understood. The objective of this study was to evaluate, in vitro, the effect of different methods used in dental prophylaxis on the mineral content of demineralized enamel surfaces, using quantitative light-induced fluorescence (QLF). Extracted sound premolars were used (n=40). The sample was made up of 4 groups: G1 - Robinson brush and pumice stone; G2 - Robinson brush and prophylactic paste; G3 - Rubber and pumice bowl; G4 - Rubber cup and prophylactic paste. The evaluations were carried out at 3 levels: with the tooth healthy, immediately after the demineralization process and after application of the proposed treatments. The primary variable analyzed was the mineral content of the enamel, quantified using QLF. The data did not meet the assumptions for parametric tests, so the Friedman variance test was applied using version 5.0 of the BioEstat program. The significance level adopted was 5%. In Group I, a statistically significant difference (p<0.001) was observed between the mineral content of the sound tooth and after demineralization, indicating a white spot formation, but no significant changes (p=0.082) post-treatment with a Robinson brush and pumice stone. Group II showed similar results. Group III exhibited significant changes (p<0.001) post-demineralization and improvement (p<0.001) with a rubber cup and pumice stone. Group IV also showed significant demineralization (p<0.001) and partial remineralization (p<0.001) with a rubber cup and prophylactic paste, indicating that these treatments can improve mineral content in demineralized enamel. In conclusion, treatments using a rubber cup with pumice stone or prophylactic paste resulted in partial remineralization of demineralized enamel, while treatments using a Robinson brush did not cause significant changes in mineral content.
References
Fux C.A., Costerton J.W., Stewart P.S., Stoodley P. Survival strategies of infectious biofilms. Trends Microbiol. 2005; 13 (1): 34-40.
Hall-Stoodley L., Stoodley P. Biofilm formation and dispersal and the transmission of human pathogens. Trends Microbiol. 2005; 13 (7): 300-1.
Yasunaga H., Takeshita T., Shibata Y., Furuta M., Shimazaki Y., Akifusa S., Ninomiya T., Kiyohara Y., Takahashi I., Yamashita Y. Exploration of bacterial species associated with the salivary microbiome of individuals with a low susceptibility to dental caries. Clin Oral Investig. 2017; 21 (8): 2399-2406.
Havsed K., Stensson M., Jansson H., Carda-Diéguez M., Pedersen A., Neilands J., Svensäter G., Mira A. Bacterial Composition and Metabolomics of Dental Plaque From Adolescents. Front Cell Infect Microbiol. 2021; 11: 716493.
Wu S.S., Yap A.U., Chelvan S., Tan E.S. Effect of prophylaxis regimes on surface roughness of glass ionomer cements. Oper Dent. 2005; 30 (2): 180-4.
Rather S.A., Sharma S.C., Mahmood A. pH dependent effects of sodium ions on dextransucrase activity in Streptococcus mutans. Biochem Biophys Rep 2019; 7 (20): 100692.
Fejerskov O. Concepts of dental caries and their consequences for understanding the disease. Community Dent Oral Epidemiol. 1997; 25 (1) 5-12.
Giacaman R.A., Fernández C.E., Muñoz-Sandoval C., León S., García-Manríquez N., Echeverría C., Valdés S., Castro R.J., Gambetta-Tessini K. Understanding dental caries as a non-communicable and behavioral disease: Management implications. Front Oral Health. 2022;24;3:764479.
Grawish M.E., Grawish L.M., Grawish H.M., Grawish M.M., Holiel A.A., Sultan N., El-Negoly S.A. Demineralized Dentin Matrix for Dental and Alveolar Bone Tissues Regeneration: An Innovative Scope Review. Tissue Eng Regen Med 2022; 19 (4): 687-701.
Veeramani R., Shanbhog R., Bhojraj N., Kaul S., Anoop N.K. Evaluation of mineral loss in primary and permanent human enamel samples subjected to chemical demineralization by international caries detection and assessment system II and quantitative light-induced fluorescence™: An in vitro study. J Indian Soc Pedod Prev Dent. 2020; 38 (4): 355-360.
Yan J., Yang H., Luo T., Hua F., He H. Application of Amorphous Calcium Phosphate Agents in the Prevention and Treatment of Enamel Demineralization. Front Bioeng Biotechnol. 2022; 13; 10: 853436.
García Rodenas L., Palacios J.M., Apella M.C., Morando P.J., Blesa M.A. Surface properties of various powdered hydroxyapatites. J Colloid Interface Sci. 2005; 1; 290 (1): 145-54.
Lee Y.K. Fluorescence properties of human teeth and dental calculus for clinical applications. J Biomed Opt. 2015; 20 (4): 040901.
Hoffmann L., Feraric M., Hoster E., Litzenburger F., Kunzelmann K.H. Investigations of the optical properties of enamel and dentin for early caries detection. Clin Oral Investig. 2021; 25 (3): 1281-1289.
Jablonski-Momeni A., Korbmacher-Steiner H., Heinzel-Gutenbrunner M., Jablonski B., Jaquet W., Bottenberg P. Randomized in situ clinical trial investigating self-assembling peptide matrix P11-4 in the prevention of artificial caries lesions. Sci Rep. 2019; 22; 9 (1): 269.
Chong L.Y., Clarkson J.E., Dobbyn-Ross L. Bhakta S. Slow-release fluoride devices for the control of dental decay. Cochrane Database Syst Rev. 2018; 1; 3 (3): CD005101.
Bijle M.N.A., Ekambaram M., Lo E.C., Yiu C.K.Y. The combined enamel remineralization potential of arginine and fluoride toothpaste. J Dent. 2018; 76: 75-82.
O'Connell L.M., Santos R., Springer G., Burne R.A., Nascimento M.M., Richards V.P. Site-Specific Profiling of the Dental Mycobiome Reveals Strong Taxonomic Shifts during Progression of Early-Childhood Caries. Appl Environ Microbiol. 2020; 18; 86 (7): e02825-19.
Garyga V., Pochelu F., Thivichon-Prince B., Aouini W., Santamaria J., Lambert F., Maucort-Boulch D., Gueyffier F., Gritsch K., Grosgogeat B. GoPerio - impact of a personalized video and an automated two-way text-messaging system in oral hygiene motivation: study protocol for a randomized controlled trial. Trials. 2019; 10; 20 (1): 699.
Honório H.M., Rios D., Abdo R.C., Machado M.A. Effect of different prophylaxis methods on sound and demineralized enamel. J Appl Oral Sci 2006; 14: 117-23.
Kim M.J., Noh H., Oh H.Y. Efficiency of professional tooth brushing before ultrasonic scaling. Int J Dent Hyg. 2015; 13 (2): 125-31.
Eggmann F., Ayub J.M., Conejo J., Blatz M.B. Deep margin elevation-Present status and future directions. J Esthet Restor Dent. 2023; 35 (1): 26-47.
Gomes I.A., Mendes H.G., Filho E.M.M., de C. Rizzi C., Nina M.G., Turssi C.P., Vasconcelos A.J., Bandeca M.C., de Jesus Tavarez R.R. Effect of Dental Prophylaxis Techniques on the Surface Roughness of Resin Composites. J Contemp Dent Pract. 2018; 19 (1): 37-41.
Bailey L.R., Phillips R.W. Effect of certain abrasive materials on tooth enamel. J Dent Res. 1950; 29: 740-8.
Boyde, A. Airpolishing effects on enamel, dentine, cement and bone. J Br Dent. 1984; 156: 287-91.
Salami D., Luz M.A.Effect of prophylactic treatments on the superficial roughness of dental tissues and of two esthetic restorative materials.Search. Dentistry. Brazil. 2003; 17: 63-8.
Honório H. M., Rios D., Abdo R.C., Machado M.A. Effect of different prophylaxis methods on sound and demineralized enamel. J Appl Oral Sci. 2006; 14: 117-23.
Marquezan, M. Artificial methods of dentine caries inducing a hardness and morphological comparative study. Oral Biology. 2009; 54: 1111-7.
De Oliveira, A.L.B.M., Giro, E.M.A., Garcia, P.P.N.S., Campos, J. Á. D.B., Phark, J.H., & Duarte Jr., S. (2014). Roughness and morphology of composites: influence of type of material, fluoride solution, and time. Microscopy and Microanalysis, 20 (5), 1365-1372.
Pretty I.A., Hall A.F., Smith P.W., Edgar W.M., Higham S.M. The intra- and inter-examiner reliability of quantitative light-induced fluorescence (QLF) analyses. J Br Dent. 2002; 193: 105-9.
Thylstrup A., Fejerskov O. Clinical and pathological features of dental caries. In: Thylstrup A, Fejerskov O. Clinical cariology. São Paulo: Santos. 1994; 114-26.
Marquezan, M. Artificial methods of dentine caries inducing a hardness and morphological comparative study. Oral Biology. 2009; 54: 1111-7.
Al-Khateeb S., Forsberg C.M., De Josselin De Jong E., Angmar-Maênsson B. A longitudinal laser fluorescence study of white spot lesions in orthodontic patients. J Orthod Dentofac Orthop. 1998; 113: 595-602.
Al-Khateeb S., Oliveby A., De Josselin De Jong E., Angmar-Maênsson B. Laser fluorescence quantification of remineralization in situ of incipient enamel lesions: Influence of fluoride supplements. Caries Res. 1997; 31: 132-40.
Al-Khateeb S., Ten Cate J.M., Angmar-Maênsson B., De Josselin De Jong E., Sundstroèm G., Exterkate R.A.M., Oliveby A. Quantification of formation and remineralization of artificial enamel lesions with a new portable fluorescence device. Adv Dent Res. 1997; 4: 502-6.
Al-Mulla A., Karlsson L., Kharsa S., Kjellberg H., Birkhed D. Combination of high-fluoride toothpaste and no post-brushing water rinsing on enamel demineralization using an in-situ caries model with orthodontic bands. Acta Odontol Scand. 2010; 68: 323-8.
Tranñus S., Al-Khateeb S., BjoÈrkman S., Twetman S., Angmar-MaÊnsson B. Application of quantitative light-induced fluorescence to monitor incipient lesions in caries-active children. A comparative study of remineralization by fluoride varnish and professional cleaning. Eur J Oral Sci. 2001; 109: 71-5.
Covey D.A., Barnes C., Watanabe H., Johnson W.W. Effects of a paste-free prophylaxis polishing cup and various prophylaxis polishing pastes on tooth enamel and restorative materials. Gen Dent. 2011; 59: 466-73.
Kimyai S., Savadi-Oskoee S., Ajami A.A., Sadr A., Asdagh S. Effect of three prophylaxis methods on surface roughness of giomer.Med Oral Patol Oral Cir Bucal. 2011; 16: 110-4.