Odovtos International Journal of Dental Sciences ISSN Impreso: 1659-1046 ISSN electrónico: 2215-3411

OAI: https://revistas.ucr.ac.cr/index.php/Odontos/oai
A Protocol to Perform Systemic Lipopolysacharide (LPS) Challenge in Rats
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Ramírez DDS, MSc, PhD, K., Quesada-Yamasaki MLS, D., & Fornaguera-Trías PhD, J. (2019). A Protocol to Perform Systemic Lipopolysacharide (LPS) Challenge in Rats. Odovtos International Journal of Dental Sciences, 21(1), 53–66. https://doi.org/10.15517/ijds.v21i1.35510

Resumen

El lipopolisacárido (LPS) es un componente de la membrana externa de las bacterias Gram negativas. En animales, la administración intraperitoneal de LPS estimula la inmunidad innata y la producción de citoquinas proinflamatorias. El LPS proporciona un estímulo inflamatorio que activa el sistema neuroinmunológico y el sistema neuroendocrino, lo que da como resultado un conjunto de respuestas denominadas conductas de enfermedad. El propósito de este protocolo es describir paso a paso la preparación y el procedimiento de aplicación de la inyección intraperitoneal de LPS en ratas, como una guía para aquellos investigadores que desean utilizar este método para estimular una respuesta inflamatoria en el animal. La estimulación con LPS en ratas, aplicada intraperitonealmente, se ha utilizado ampliamente para evaluar reactivos antiinflamatorios y para abordar preguntas básicas de investigación científica.

https://doi.org/10.15517/ijds.v21i1.35510
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