Palabras clave
Hueso; Terapia con láser de bajo intensidad; Osteoblastos; Osteocitos; Fotobiomodulación.
Cómo citar
Resumen
El objetivo de este estudio experimental fue determinar el efecto de terapia de fotobiomodulación sobre la reparación ósea en un modelo de osteotomía de tibia de rata a los 15 y 30 días. La muestra estuvo compuesta por 36 ratas Holtzman macho que se aleatorizaron en 6 grupos iguales. Grupos A1 y A2: osteotomía + energía láser de 1 Joule. Grupos B1 y B2: osteotomía + energía láser 3 Joule. Grupos C1 y C2 (controles): solo osteotomía. La reparación ósea fue analizada por evaluación histológica de osteoblastos y osteocitos tanto a los 15 días (grupos A1, B1 y C1) como a los 30 días (grupos A2, B2 y C2). Como resultados se encontró que en todos los grupos hubo mayor número de osteoblastos a los 15 días vs. 30 días (p<0,05), y mayor número de osteocitos en B1 y C2 vs B2 y C1, respectivamente (p<0,05). Al evaluar a los animales a los 15 días, se observó mayor número de osteoblastos en A1 y C1 vs B1 (p<0.001); y mayor número de osteocitos en A1 y B1 vs C1 (p<0,001). Al evaluar a los ratones a los 30 días hubo mayor cantidad de osteoblastos en C2 vs A2 y B2 (p<0,05) y de osteocitos en C2 vs B2 (p<0,05). Se concluye que la terapia de fotobiomodulación con 1 Joule mejoró la reparación ósea a los 15 días; sin embargo, dicha mejora no se observó a los 30 días porque no hubo diferencias entre los grupos irradiados y el control.
Citas
Hosseinpour S., Fekrazad R., Arany P., Ye, Q. Molecular impacts of photobiomodulation on bone regeneration: A systematic review. Prog Biophysd Mol Biol. 2019; 149: 147-159
Nica D., Heredea E., Todea D. Alveolus soft and bone tissue regeneration after laser biomodulation - a histological study. Rom J Morphol Embryol. 2019; 60 (4): 1269-1273.
Karu T.I. Multiple roles of cytochrome c oxidase in mammalian cells under action of red and IR-A radiation. IUBMB Life. 2010; 62 (8): 607-610.
De Freitas L., Hamblin M. Proposed Mechanisms of Photobiomodulation or Low-Level Light Therapy. IEEE J Sel Top Quantum Electron. 2016; 22 (3): 7000417.
Hamblin M. Mechanisms and applications of the anti-inflammatory effects of photobiomodulation. AIMS biophysics. 2017; 4 (3): 337-361.
Ustaoglu G., Ercan E., Tunali, M. Low-level laser therapy in enhancing wound healing and preserving tissue thickness at free gingival graft donor sites: a randomized, controlled clinical study. Photomed Laser Surg. 2017; 35: 223-230.
Dostalova T., Kroulikova V., Podzimek S., Jelinkov H. Low-level laser therapy after wisdom teeth surgery: evaluation of immunologic markers (secretory immunoglobulin and lysozyme levels) and thermographic examination: placebo controlled study. Photomed Laser Surg. 2017; 35: 616-621.
Escudero J., Perez M., de Oliveira Rosso M., Buchaim V., Pomini K., Campos L., Audi M., Buchaim R. Photobiomodulation therapy (PBMT) in bone repair: A systematic review. Injury. 2019; 50 (11): 1853-1867.
Hanna R., Dalvi S., Amaroli A., De Angelis N., Benedicenti S. Effects of photobiomodulation on bone defects grafted with bone substitutes: A systematic review of in vivo animal studies. J Biophotonics. 2021; 14 (1): e202000267.
Chumpitaz Cerrate V., Franco Quino C., Aguirre Siancas E. Influence of ambient oxygen pressure on guided bone regeneration. Rev. Clin. Periodoncia Implantol. Rehabil. Oral. 2017; 10 (2): 111-114.
Setiawati R., Rahardjo P. Bone Development and Growth. In Osteogenesis and Bone Regeneration. Londres: IntechOpen; 2018.
De Almeida J., De Moraes R., Gusman D., Faleiros P., Nagata M., Garcia V., Theodoro L., Bosco A. Influence of low-level laser therapy on the healing process of autogenous bone block grafts in the jaws of systemically nicotine-modified rats: A histomorphometric study. Arch Oral Biol. 2017; 75: 21-30.
Zaky A., El Shenawy H., Harhsh T., Shalash M., Awad N. Can Low Level Laser Therapy Benefit Bone Regeneration in Localized Maxillary Cystic Defects? - A Prospective Randomized Control Trial. Open access Maced J Med. 2016; 4 (4): 720-725.
Amaroli A., Agas D., Laus F., Cuteri V., Hanna R., Sabbieti M., Benedicenti S. The Effects of Photobiomodulation of 808 nm Diode Laser Therapy at Higher Fluence on the in Vitro Osteogenic Differentiation of Bone Marrow Stromal Cells. Front Physiol. 2018; 9: 123.
Hanna R., Agas D., Benedicenti S., Ferrando S., Laus F., Cuteri V., Lacava G., Sabbieti M., Amaroli A. A Comparative Study Between the Effectiveness of 980 nm Photobiomodulation Delivered by Hand-Piece With Gaussian vs. Flat-Top Profiles on Osteoblasts Maturation. Front Endocrinol (Lausanne). 2019; 10: 92.
De Paiva P., Casalechi H., Tomazoni S., Machado C., Miranda E., Ribeiro N., Pereira A., da Costa A., Dias L., Souza B., Aguiar Lino M., de Carvalho P. & Leal-Junior E. Effects of photobiomodulation therapy in aerobic endurance training and detraining in humans: Protocol for a randomized placebo-controlled trial. Medicine (Baltimore). 2019; 98 (18): e15317.
Jenkins P., Carroll J. How to Report Low-Level Laser Therapy (LLLT)/Photomedicine Dose and Beam Parameters in Clinical and Laboratory Studies. Photomed Laser Surg. 2011; 29 (12): 785-787.
Llapapasca Cruz C., De la Torre F., Jiménez Sánchez S., Mallma Medina A., Ruiz Ramirez E., Valdez Jurado F. Efecto del láser terapéutico infrarrojo en la reparación ósea post-exodoncia en ratas albinas. Rev. Estomatol. Herediana. 2017; 27 (2): 101-110.
Atasoy K.T., Korkmaz Y.T., Odaci E., Hanci H. The efficacy of low-level 940 nm láser therapy with different energy intensities on bone healing. Braz. Oral Res. 2017; 31: 1-9.
Çırak E., Özyurt A., Peker T., Ömeroğlu S., Güngör M. Comparative evaluation of various low-level laser therapies on bone healing following tooth extraction: An experimental animal study. J Craniomaxillofac Surg. 2018; 46 (7): 1147-1152.
Desai S., Mudda J., Patil V., Maharudrappa, Satish B. Effect of irradiation of 810nm laser on bone for 10 sec: A rabbit histological study. IOSR-JDMS. 2019; 18 (3): 14-20.
Abdel Hamid M., Zaied A., Zayet M., Abdelmageed H., Hassan E., Amaroli A. Efficacy of Flat-Top Hand-Piece Using 980 nm Diode Laser Photobiomodulation on Socket Healing after Extraction: Split-Mouth Experimental Model in Dogs. Photochem Photobiol. 2020; 97: 627-633.
Brito G., Macedo F., Moura J., de Carvalho M. Effectiveness of gallium and aluminum Arsenide laser in bone repair. BJHR. 2021; 4 (2): 5301-5315.
Tim C.; Bossini P.; Kido H., Malavazi I., Von Zeska Kress M.; Carazzolle M., Parizotto N., Rennó A. Effects of low-level laser therapy on the expression of osteogenic genes during the initial stages of bone healing in rats: a microarray analysis. Lasers Med Sci. 2015; 30 (9): 2325-2333.
Carneiro V.S.M., Jr. F.dA.L., Gerbi M.E.M., Menezes R.F.d., SantosNeto A.Pd., Araújo N.C. Diode λ830nm laser associated with hydroxyapatite and biological membranes: bone repair in rats. 2016. Proc. SPIE 9692, Lasers in Dentistry XXII.
Fernandes K.R., Magri A.M.P., Kido H.W., et al. Biosilicate/PLGA osteogenic effects modulated by laser therapy: in vitro and in vivo studies. J Photochem Photobiol B. 2017; 173: 258-265.
Gabbai-Armelin P.R., Caliari H.M., Silva D.F., et al. Association of Bioglass/collagen/magnesium composites and low level irradiation: effects on bone healing in a model of tibial defect in rats. Laser Ther. 2018; 27 (4): 271-282.
De Oliveira G., Aroni M., Medeiros M., Marcantonio E., Marcantonio R. Effect of low-level laser therapy on the healing of sites grafted with coagulum, deproteinized bovine bone, and biphasic ceramic made of hydroxyapatite and β-tricalcium phosphate. In vivo study in rats. Lasers Surg. Med. 2018; 50 (6): 651-660.
Yılmaz B., Akman A., Çetinkaya A., Colak C., Yildirim B., Yucel O., Guncu G. & Nohutcu R. In vivo efficacy of low-level laser therapy on bone regeneration. Lasers Med Sci. 2022; 37: 2209-2216
Chumpitaz-Cerrate V., Chávez-Rimache L., Franco-Quino C., Aguirre-Siancas E., Caldas-Cueva V., Ruiz Ramirez, E. Effects of NSAIDs and environmental oxygen pressure on bone regeneration. J Oral Res 2019; 8 (2): 152-158.
Esteves J.C., de Souza Faloni A.P., Macedo P.D., Nakata P.B., Chierici Marcantonio R.A., Intini G, Marcantonio E. Effects on Bone Repair of Osteotomy With Drills or With Erbium, Chromium: Yttrium-Scandium-Gallium-Garnet Laser: Histomorphometric and Immunohistochemical Study. J Periodontol. 2016, 87 (4): 452-460.
Chumpitaz-Cerrate V., Chávez-Rimache L., Aguirre-Siancas E., Franco-Quino C., Ruiz-Ramirez E., Paredes-Erazo C. Biochemical and histopathological effects of diclofenac and ketoprofen administration on bone regeneration. Braz Dent Sci. 2021; 24 (3): 1-8.
Chumpitaz-Cerrate V., Chávez-Rimache L., Franco-Quino C., Ruiz Ramirez, E., Aguirre-Siancas E., Paredes Erazo C. Effects of salbutamol, montelukast and prednisone on orthodontic tooth movement in rats. Braz Dent Sci. 2021; 24 (2): 1-9.
Comentarios
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-CompartirIgual 4.0.
Derechos de autor 2023 CC-BY-NC-SA 4.0