Agronomía Costarricense ISSN Impreso: 0377-9424 ISSN electrónico: 2215-2202

OAI: https://revistas.ucr.ac.cr/index.php/agrocost/oai
Identificación molecular del hongo <i>Colletotrichum spp</i>., aislado de banano (<i>Musa spp</i>) de altura en la zona de Turrialba y determinación de su sensibilidad a fungicidas poscosecha
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Palabras clave

antracnosis
pcr
poscosecha
tiabendazole
imazalil
miclobutanil
anthracnose
pcr
postharvest
thiabendazole
imazalil
miclobutanil

Cómo citar

Salazar, E., Hernández, R., Tapia, A., & Gómez-Alpízar, L. (2013). Identificación molecular del hongo <i>Colletotrichum spp</i>., aislado de banano (<i>Musa spp</i>) de altura en la zona de Turrialba y determinación de su sensibilidad a fungicidas poscosecha. Agronomía Costarricense, 36(1). https://doi.org/10.15517/rac.v36i1.9964

Resumen

La antracnosis, causada por el hongo Colletotrichum spp., es una de las principales enfermedades poscosecha que afectan el fruto del banano. En el presente experimento, 25 aislamientos de Colletotrichum spp., obtenidos de frutos de banano provenientes de 5 zonas del cantón de Turrialba, Costa Rica, fueron utilizados para caracterizar molecularmente al hongo y evaluar su sensibilidad in vitro a 3 fungicidas poscosecha: tiabendazol, imazalil y miclobutanil. La identificación molecular de cada uno de los aislamientos se realizó mediante la técnica PCR con los imprimadores especie-específicos de la región ITS (CgInt/ITS5) y de una región del gen de la ß-tubulina (TBCG/ TB5). Para las pruebas de sensibilidad in vitro a los 3 fungicidas, se utilizaron las siguientes concentraciones: 0,1; 1,0; 10,0 y 100,0 μg.ml-1. El análisis de la región ITS y de la región del gen de la β-tubulina, indicó que los aislamientos analizados corresponden a la especie Colletotrichum gloeosporioides. En cuanto a las pruebas de sensibilidad in vitro, todos los aislamientos fueron sensibles al tiabendazol, mientras que existe una marcada insensibilidad al imazalil y al miclobutanil.

https://doi.org/10.15517/rac.v36i1.9964
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Citas

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